The aim of this study was to analyse the prevalence of human papillomavirus (HPV) infection among patients with oral and oropharynx squamous cell carcinoma (OSCC). The correlation between HPV infection and OSCC, HPV genotypes, and correlation between HPV, OSCC, alcohol use, tobacco smoking, demographic data (gender, age, place of residence), anatomic location, pretreatment staging, metastases of lymph node evidence, and grading was investigated. In the examination group, there were 60 patients with squamous cell carcinoma (SCC), 54 males and six females. Twenty-one patients were affected with oral, 24 - with oropharynx, and 15 - with oral and oropharynx SCC. The patients were not subjected to chemotherapy and radiotherapy before operation. The examination samples were collected from paraffin sections. This analysis involved DNA isolation from histological specimens, an amplification of a fragment of human ß-globin gene (reference gene), electrophoresis of amplification products of the gene, a quantitative DNA analysis (using spectrophotometry method), PCR, and genotyping. HPV DNA was detected in 25% of patients with SCC. Among HPV positive patients, 86.7% of the patients were infected with HPV type 16 and 13.3% were infected with another non identified HPV.
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