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Agrobacterium - mediated genetic transformation of Withania somnifera using nodal explants

100%

Udayakumar R., Kasthurirengan S., Mariashibu T. S., Sahaya Rayan J. J., Ganapathi A., Kim S. C., Kim J. J., Choi C. W.

Acta Physiologiae Plantarum

2014

tom 36

nr 08

Withania somnifera is an important medicinal plant and used to cure many diseases. Direct regeneration method was standardized for the nodal explants of W. somnifera. In this method, the maximum of 42.4 ± 2.68 shoots produced per explant was achieved at 1.5 mg l⁻¹ BAP with 0.3 mg l⁻¹ IAA in the second subculture. Transformation was performed in the nodal explants of W. somnifera via direct regeneration using Agrobacterium tumefaciens strain EHA105 that harbored a binary vector pGA492, which carrying kanamycin resistant (nptII), phosphinothricin resistant (bar) and an intron containing β-glucuronidase (gus-intron) genes. The sensitivity of nodal explants to kanamycin (300 mg l⁻¹) was determined for the selection of transformed plants. Transformation was confirmed by histochemical β-glucuronidase (GUS) assay and amplification of the nptII gene by polymerase chain reaction (PCR). PCR and southern blot analyses confirmed the integration of nptII gene in the genome of W. somnifera and the transformation frequency of 3.16 % has been achieved. This is the first report on the genetic transformation of W. somnifera using nodal explants, which may aid in the transformation of any other character gene for improving therapeutic value.

Influence of protein supply on threonine efficiency and threonine catabolism in hepatic mitochondria of chicks and rats

100%

Lee C. W., Zhao R., Cho I. J., Byun S. H., Kim Y. W., Kim Y. S., Kim S. C., An W. G.

Annals of Animal Science

2016

tom 16

nr 1

The human ether-a’-go-go related gene (hERG) Kplus channel blockade by the investigative selective-serotonin reuptake inhibitor CONA-437: limited dependence on S6 aromatic residues

100%

Alexandrou A. J., Milnes J. T., Sun S. Z., Fermini B., Kim S. C., Jenkinson S., Leishman D. J., Witchel H. J., Hancox J. C., Leaney J.

Journal of Physiology and Pharmacology

2014

tom 65

nr 4

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Annals of Animal Science

1 Journal of Physiology and Pharmacology

3 Kim S. C.

1 Alexandrou A. J.

1 An W. G.

1 Byun S. H.

1 Cho I. J.

1 Choi C. W.

1 Fermini B.

1 Ganapathi A.

1 Hancox J. C.

1 Jenkinson S.

1 Kasthurirengan S.

1 Kim J. J.

1 Kim Y. S.

1 Kim Y. W.

1 Leaney J.

1 Lee C. W.

1 Leishman D. J.

1 Mariashibu T. S.

1 Milnes J. T.

1 Sahaya Rayan J. J.

1 Sun S. Z.

1 Udayakumar R.

1 Witchel H. J.

1 Zhao R.

1 2016

2 2014

Wyniki wyszukiwania

Agrobacterium - mediated genetic transformation of Withania somnifera using nodal explants

Influence of protein supply on threonine efficiency and threonine catabolism in hepatic mitochondria of chicks and rats

The human ether-a’-go-go related gene (hERG) Kplus channel blockade by the investigative selective-serotonin reuptake inhibitor CONA-437: limited dependence on S6 aromatic residues