Wyniki wyszukiwania

1

Circadian rhythm of sperm release in male cotton leafworm - Spodoptera littoralis; temporal distribution of per protein in male reproductive system

100%

Bebas P.

Acta Neurobiologiae Experimentalis

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1999

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tom 59

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nr 3

2

O złożoności zegara biologicznego owadów, czyli jak narządy odmierzają czas

100%

Bebas P.

Kosmos

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2010

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tom 59

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nr 3-4

3

Functions of biological clocks in insect physiology and reproduction: Practical aspects

63%

Giebultowicz J. M., Bebas P.

Pestycydy

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2005

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nr 4

77-90

4

Struktura oscylatora biologicznego owadów

63%

Bebas P., Kotwica J.

Prace i Materiały Zootechniczne

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2008

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tom 66

5

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Propable circadian rhythms of serotonin concentartion in the haemolymph of Unio tumidus and the tissues of Pisidium casertanum

51%

Skawina A., Bernatowicz P., Markowska M., Bebas P.

Folia Malacologica

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2015

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tom 23

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nr 1

6

Owady i ich rola w przyrodzie - kształtowanie wiedzy i postaw uczniów (2)

45%

Potyrala K., Czerwiec K., Klys M., Bebas P., Chrzanowski M. M.

Aura

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2016

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nr 11 Dodatek Ekologiczny dla Szkół nr 265

7

Owady i ich rola w przyrodzie - kształtowanie wiedzy i postaw uczniów

45%

Potyrala K., Czerwiec K., Klys M., Bebas P., Chrzanowski M. M.

Aura

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2016

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nr 10 Dodatek Ekologiczny dla Szkół nr 264

8

Kernel current source density and connectivity estimation methods for the analysis of local field potentials

45%

Sredniawa W., Jablonka J., Sadowska M., Bebas P., Wojcik D. K.

Acta Neurobiologiae Experimentalis

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2017

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tom 77

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nr Suppl.1

INTRODUCTION: Extracellular potentials, such as the Local Field Potentials (LFPs), are routinely measured in numerous electrophysiological experiments. LFP can carry valuable information about the electric properties of the tissue, however analysis of the recorded signal is usually a complex task. Apart from basic preparation, such as bandpass filtering and artifact removal, many other analytic methods have been proposed for the LFP study. Here we discuss methods for estimation of electric sources and sinks in brain tissue (Current Source Density, CSD) and methods to estimate connectivity in small networks, and their utility in analysis of cortical recordings in rats. AIM(S): Comparison of the effective connectivity and the structure of sinks and sources in cortical columns during whisker stimulations. METHOD(S): Analytic methods: kernel Current Source Density and Modular Connectivity Factorization (MCF) applied to LFP recordings and simulated data from cortical column. Experimental methods: Simultaneous multielectrode in vivo recordings from both hemispheres of the rats brain. RESULTS: Preliminary studies show different distribution of the current sources in contralateral to ipsilateral hemisphere during whisker stimulation in rats. Comparison of the hemispheres from deprived rats shows an extension of the whisker representation in the barrel cortex receptive field. CONCLUSIONS: KCSD method showssignificant differences in current sources localization in contralateral to ipsilateral hemisphere. Modular Connectivity Factorization method applied to LFP recordings from simulated data separates cortical column layers into interpretable modules. Physiological interpretation of the results needs further validation on the cortical column model. FINANCIAL SUPPORT: Instytut Biologii Doświadczalnej im. Marcelego Nenckiego Polska Akademia Nauk, Warsaw, Poland, Uniwersytet Warszawski, Warsaw, Poland

9

Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock

39%

Kotwica J., Ciuk M. A., Joachimiak E., Rowinski S., Cymborowski B., Bebas P.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 8

107-123

The male reproductive tract of Lepidoptera is an ideal model for the study of the physiological role of peripheral clocks in insects. The latter are significant in the generation and coordination of rhythmic phenomena which facilitate the initial stages of sperm capacitation. This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase. Our aim was to determine the potential involvement of carbonic anhydrase (CA) in this process, an enzyme tasked with generating protons subsequently utilized by H+-ATPase to acidify the UVD milieu in S. littoralis, during the time when the lumen of this organ is filled with sperm. We attempted to answer the question whether CA activity can be controlled by the biological oscillator present in the male reproductive tract of the cotton leafworm. Using PAGE zymography, the presence of CA was demonstrated in the UVD wall, but not in the luminal fluid nor in the sperm. Using histochemistry, it was shown that CA is active in the UVD epithelium, and that this activity varies throughout the day and is most likely controlled by an endogenous biological clock. Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.

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