Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Striking pattern of Purkinje cell loss in cerebellum of an ataxic mutant mouse, tottering

100%

Sawada K., Azad A. K., Sakata-Haga H., Lee N.-S., Jeong Y.-G., Fukui Y.

Acta Neurobiologiae Experimentalis

2009

tom 69

nr 1

Tottering mouse is an ataxic mutant that carries a mutation in a gene encoding for the α1A subunit of P/Q-type Ca2+ channel (Cav2.1). This study revisited to examine whether a Purkinje cell loss occurred in the cerebellum of tottering mice. In tottering mice, Calbindin D-28k negative gaps were apparent in the vermis but not in the hemisphere. Calbindin D-28k immunofluorescence with DAPI staining demonstrated the absence of Purkinje cells in the Calbindin D-28k negative gaps. The Purkinje cell loss seemed to be observed prominently in the zebrin II negative compartments of the anterior vermis, but in the zebrin II positive compartments of the posterior vermis. Quite consistent with the histopathological observations, quantitation of the density of Calbindin D-28k and zebrin II immunopositive Purkinje cells in the tottering cerebellum revealed that the Purkinje cells were selectively lost in the zebrin II immunonegative compartments of the lobules I and II but in the zebrin II immunopositive compartments in the lobule IX. Those results predict that the susceptibility to the Cav2.1 gene defect is different among Purkinje cell phenotypes of the tottering cerebellum rather than the expression pattern of mutated Cav2.1 channels. This may result in the reproducible parasagittal pattern of Purkinje cell loss.

Correlation between formation of the calcarine sulcus and morphological maturation of the lateral ventricle in cynomolgus monkey fetuses

84%

Fukunishi K., Sawada K., Kashima M., Saito S., Sakata-Haga H., Sukamoto T., Aoki I., Fukui Y.

Acta Neurobiologiae Experimentalis

2011

tom 71

nr 3

In the present study developmental changes in the cerebral sulci and volumes of subcortical and archicortical structures of the cerebrum in cynomolgus monkey fetuses were examined with Tj-weighted magnetic resonance (MR) images in 3D. On the embryonic day (ED) 90, the lateral ventricle had still an immature vesicular shape in the occipital region of the cerebrum, and it dramatically closed its lumen by ED 100. In that period the calcarine sulcus progressively infolded from the medial surface of the cerebral hemisphere narrowing the lumen of the lateral ventricle in the occipital region. Volume of the lateral ventricle decreased in the period ED 90-100, increasing afterwards in spite of increasing volumes of subcortical and archicortical structures such as the caudate nucleus, putamen, globus pallidus, amygdala and hippocampal formation. During the same time, the volume of the germinal matrix around lateral ventricles decreased to disappear completely by ED 120. These results suggest that the morphological maturation of lateral ventricle is linked to the development of calcarine sulcus in cynomolgus monkey fetuses. The degree of infolding of calcarine sulcus on ED 100 would be useful as a gross anatomical landmark for evaluating the cerebral maturation in cynomolgus monkey fetuses.

Ograniczanie wyników

2 Acta Neurobiologiae Experimentalis

2 Fukui Y.

2 Sakata-Haga H.

2 Sawada K.

1 Aoki I.

1 Azad A. K.

1 Fukunishi K.

1 Jeong Y.-G.

1 Kashima M.

1 Lee N.-S.

1 Saito S.

1 Sukamoto T.

1 2011

1 2009

Wyniki wyszukiwania

Striking pattern of Purkinje cell loss in cerebellum of an ataxic mutant mouse, tottering

Correlation between formation of the calcarine sulcus and morphological maturation of the lateral ventricle in cynomolgus monkey fetuses