Cuticle visualized by auramine O fluorescence on developing Arabidopsis thaliana embryos was investigated. Localization of the cuticle was studied on embryos of the zygotic wild Ler ecotype and nine lines of embryonic mutant: CS 2330, CS 3009, CS 3016, CS 3023, CS3025, CS 6330, CS 6340, CS 6343 and CS 6346. In Arabidopsis Ler ecotype embryogenesis, a fluorescing cuticle layer appears on the globular embryo and persists during successive stages of development. Such a layer does not occur on the suspensor. In a similar way, fluorescing cuticle envelops the entire globular and older embryo of embryonic mutants, although the embryos of different mutant lines reach different developmental stages.
A total of 103 strains of Aeromonas spp. isolated from clinical and from environmental samples was compared by using SDS-PAGE of periplasms proteins patterns. Strains isolated from Polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in Hong-Kong. Aeromonas sp. strains did not show a tendency to cluster according to their origin. Our results have demonstrated no species-specifs periplasms protein profiles. A significant protein electrophoretic heterogeneity was observed within the species A. hydrophila, A. hestiarum, A. salmonicida, A. caviae, A. media, and A. vermin biotype sobria.
In vitro organogenesis was studied using Citrus limon L. Burm cv. ‘Primofiore’ leaf explants. The purpose of the present study was to optimize conditions for callogenesis and organogenesis of C. limon. Explants of C. limon were cultured on 16 different media supplemented with various combinations of plant growth regulators, both auxins and cytokinins, such as 6-benzylaminopurine (BAP), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin. The best shoot induction was obtained when the leaf explants were cultured on Murashige & Tucker media supplemented with 3.5 mg·L-1 BAP. Histological investigation revealed most likely the initial phase of development of leaf explants during in vitro regeneration of C. limon. t