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1

Isilation of a diaminobutyric acid-degrading bacterium from the sheep rumen

100%
Peng H., Revell D., Brooker J. D.
Journal of Animal and Feed Sciences. Supplement
|
2007
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tom 16
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nr 2
2

Predicting current and future invasion of Solidago canadensis: a study from China

100%
Xu Z., Peng H., Feng Z., Abdulsalih N.
Polish Journal of Ecology
|
2014
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tom 62
|
nr 2
Solidago canadensis, which is native to North America, is considered to be the most widespread invasive alien plant. The invasion of Solidago canadensis in China has resulted in serious environmental problems. Therefore, understanding the relationship between the geographical distribution of S. canadensis and bioclimatic variables, and then predicting the potential distribution of this species is essential for management actions and practices. Although several studies have delineated the potential distribution of S. canadensis in China, how this species would respond to variations in future climatic conditions remains unclear. In the present study, we predicted the potential distribution of S. canadensis under current and future climatic conditions using species distribution models. We also analyzed range shifting of this species under current and future climatic conditions. We arrived at several conclusions. First, the potential distribution of S. canadensis may expand 40% under future climatic condition compare with that of under current condition. Second, mean diurnal range, isothermality, mean temperature of the wettest quarter, mean temperature of the warmest quarter, precipitation of the driest month, and precipitation seasonality (coefficient of variation) are key bioclimatic variables in determine the potential distribution of S. canadensis. Third, expansion of S. canadensis can be partly attributed to the relatively warmer and wetter future bioclimatic condition than current one.
3

Spatial variability of soil physical properties based on GIS and geo-statistical methods in the red beds of the Nanxiong Basin, China

100%
Yan P., Peng H., Yan L., Lin K.
Polish Journal of Environmental Studies
|
2019
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tom 28
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nr 4
Spatial variability of soil has an important influence on the structure and function of soil. The spatial distribution of soil physical properties provides basic and useful information relevant to soil management and ecological protection. A typical red beds basin was selected for this study, soil samples at 0-20 cm were taken from 150 locations in the northeast part of Nanxiong Basin, in which GIS and geostatistics were used to analyze the spatial variability of the soil physical properties. The results show that the coefficients of variation of soil bulk density, total porosity and capillary porosity are 9.82%, 4.47%, and 3.72%, respectively, which indicate weak variation. Pearson correlation indicated that soil bulk density was significantly positively correlated with soil moisture and capillary water capacity (p<0.01), with correlation coefficients of 0.85 and 0.91, respectively, but was significantly negatively correlated with total porosity, capillary porosity and non-capillary porosity, with correlation coefficients of 0.82, 0.71 and 0.94, respectively (p<0.01). The spatial distributions of soil physical properties using ordinary kriging (OK) and empirical bayesian kriging (EBK) methods were subjected to comparative analysis. In addition, different cross-validation indicators were applied to assess the performance of different interpolation methods. Cross-validation demonstrated that EBK performed better than OK. And EBK produced smaller regions of predicted soil physical properties than OK, highlighting the necessity of choosing the appropriate methods in studying the spatial distribution of soil properties.
4
Content available remote

Evidence for state-dependent block of DPI 201-106, a synthetic inhibitor of Na+ channel inactivation, on delayed-rectifier K+ current in pituitary tumor (GH3) cells

88%
Wang Y.-J., Lin M.-W., Lin A.-A., Peng H., Wu S.-N.
Journal of Physiology and Pharmacology
|
2008
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tom 59
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nr 3
409-423
DPI 201-107 (DPI), a diphenylpiperazinylindole derivative, was reported to be a cardio-selective modifier of voltage-gated Na+ channels. It remains unclear whether DPI has any effects on ion currents. The effects of DPI on ion currents and membrane potential in pituitary tumor (GH3) cells were investigated in this study. DPI (1-100 µM) suppressed the amplitude of delayed-rectifier K+ current (IK(DR)) in a concentration-dependent manner with an IC50 value of 9.4 µM. The presence of DPI also enhanced the rate and extent of IK(DR) inactivation. Recovery from block by DPI (10 µM) was fitted by a single exponential. Crossover of tail currents during the exposure to DPI was also observed. Under current-clamp recordings, DPI prolonged action potential duration in GH3 cells. With a minimal binding scheme, DPI-induced block of IK(DR) was quantitatively provided. The exposure to DPI also blocked IK(DR) with a concomitant increase in current inactivation in NG108-15 neuronal cells. Taken together, the results imply that DPI acts as an open-channel blocker of delayed-rectifier K+ channels in these cells. The widening of action potentials induced by DPI in these cells may be explained mainly by its block of IK(DR) in a state-dependent manner.
5

MiR393-targeted TIR1-like (F-box) gene in response to inoculation to R. Solani in Zea mays

88%
Luo M., Gao J., Peng H., Pan G., Zhang Z.
Acta Physiologiae Plantarum
|
2014
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tom 36
|
nr 05
MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate special target mRNAs at the post-transcriptional level by directing target mRNA cleavage or translational inhibition. Plant miRNAs regulate gene expression mainly by guiding cleavage of target mRNAs and subsequently play important roles in diverse developmental processes, nutrient homeostasis and responses to biotic and abiotic stresses. MiRNA393 plays important and diverse roles in defense against bacterial pathogens by negatively targeting transport inhibitor response 1 (TIR1) in plant development. It will be essential for understanding complex feedback regulations in the development pathway by unraveling the miR393 network in a temporal and spatial manner. Here, we report that Zma-miR393b down-regulates its putative target TIR1-like (F-box) gene by guiding the cleavage of their mRNAs in development of leaf sheaths in response to R. Solani infection, Zma-miR393b and its putative target gene TIR1 were confirmed through Q-PCR and the spatial expression of Zma-miR393b was further analyzed by in situ hybridization. These findings suggested that, as a negative feedback regulation of TIR1-like (F-box) gene, Zma-miR393b plays an important role in defense against R. Solani infection.
6

The construction of the eukaryotic expression plasmid pcDNA3.1-azurin and the increased apoptosis of U2OS cells transfected with it

88%
Ye Z., Peng H., Fang Y., Feng J., Yang D. S.
Cellular and Molecular Biology Letters
|
2007
|
tom 12
|
nr 3
In our previous study, we demonstrated that azurin could selectively trigger apoptosis in human osteosarcoma cell line U2OS cells. However, the rate of apoptosis (35.8 ± 3.2%) is not very high, and azurin is too expensive to obtain readily. To solve these problems, we constructed a eukaryotic expression plasmid containing the azurin gene with an influenza virus haemagglutinin 9 peptide HA epitope tag, and transfected the recombinant plasmid pcDNA3.1(+)/azurin into U2OS cells. RT-PCR and Western blot analysis validated the successful transfection and the expression of the azurin-HA protein. Conspicuous apoptosis of the transfected cells was detected by flow cytometry (FCM) and the DNA ladder test. The apoptosis rate reached 64.3 ± 13.1%. The transcriptional levels of the Bax and p53 genes increased significantly in U2OS cells transfected with pcDNA3.1(+)/azurin, but the Bcl-2 mRNA level decreased. There was no difference in the levels of Bcl-xl mRNA and Survivin mRNA. We propose that the transfection of the recombinant plasmid pcDNA3.1(+)/azurin can significantly induce apoptosis in U2OS cells. This is closely associated with the up-regulation of the transcriptional level of the Bax and p53 genes, and the down-regulation of that of the Bcl-2 gene.
7

HBx and SP1 upregulate DKK1 expression

76%
Peng H., Li Y., Liu Y., Zhang J., Chen K., Huangand A., Tang H.
Acta Biochimica Polonica
|
2017
|
tom 64
|
nr 1
8

Assessment of gene flow from glyphosate-resistant transgenic soybean to conventional soybean in China

76%
Huang W.-K., Peng H., Wang G.-F., Cui J.-K., Zhu L.-F., Long H.-B., Peng D.-L.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 07
Glyphosate-resistant (GR) transgenic soybean has never been cultivated commercially in China. It is essential to develop the separation measures required to prevent out-crossing between GR and conventional soybean (Glycine max (L.) Merr.) by characterizing the transgene flow before GR soybean is released. In this study, the transgene flow from a GR transgenic soybean AG5601 to conventional soybeans was characterized. First, natural out-crossing rate was evaluated using 36 conventional soybean varieties interplanted with GR soybean AG5601 transformed with a cp4 EPSPS gene conferring the resistance to herbicide glyphosate in the field in 2007 and 2008 in China. Second, drift distance of cp4 EPSPS gene from GR soybean AG5601 to soybean cv. Zhonghuang13 was evaluated using the progenies harvested from eight directions at different distance. Third, the relationship of gene flow of GR soybean AG5601 with flowering synchronization days or insect pollinators of each variety was analyzed using regression analysis. Thirty-two of 36 tested conventional soybean varieties had surviving progenies after two times of sprays of glyphosate, and 49 of 41,679 progenies were verified to be glyphosate-tolerant heterozygous offspring. The out-crossing rates in positive varieties (having surviving offspring after two times of sprays of glyphosate) ranged from 0.039 to 0.934 %. The farthest distance (drift distance) between soybean AG5601 and cv. Zhonghuang13 at which out-pollinating was still able to be observed was 15 m, with an out-crossing rate of 0.012 %. Regression analysis showed that there was a positive relationship between cross-pollination frequency and flowering synchronization days or pollinator insects. Therefore, when GR soybean is released to the field, it should be critically separated with the conventional soybean in space and cultivation time with efficient insect control during flowering.
9

Investigating the simultaneous ecological operation of dam gates to meet the water flow requirements of fish spawning migration

76%
Zhang W., Peng H., Jia Y., Ni G., Yang Z., Zeng Q.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 3
The construction of gates in watercourses could change the original hydrological and hydrodynamic processes of drainage basins, eventually having a widespread cumulative influence on river habitat and fish resources. In this study, the hydrodynamic force in the Yongjiang River basin, China, was calculated using the MIKE FLOOD model, including water depth and flow velocity. Subsequently, the habitat conditions suitable for yellowcheek carp were used to analyse ecologically suitable flow for this target species during the spawning period at the main spawning site (Fenghua River). Different schemes of simultaneous operation of the dam gates on the Fenghua and Yaojiang rivers were simulated after gate construction on the Yongjiang River estuary, and their influence on fish spawning and migration in the main stream was investigated. During the fish spawning period, the ecological flow for facilitating yellowcheek carp spawning in the Fenghua River was 160 m³/s. The discharged water flow of gates increased to 90 and 250 m³/s, respectively, in the tributaries of the Yaojiang and Yongjiang rivers, causing the average flow velocity of the target watercourse to increase by 0.2 m/s. Based on our results, we provide recommendations for regulating water flow to enhance ecological watershed planning after gate construction along watercourses.
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