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Propofol, 2,6-diisopropylphenol, seems to be a good candidate as a fish anaesthetic, however, no study regarding propofol influence on fish has yet been reported. The aim of this study was to examine propofol pharmacokinetics in rainbow trout (Oncorhynchus mykiss) following bath exposure. Fish (n = 100) were exposed to an aqueous propofol bath at 12°C and 17°C; propofol concentration in the bath was 10 mg L(-1). Plasma concentration-time profiles were determined using LC-MS, and pharmacokinetic parameters were calculated. Propofol was absorbed quickly at both temperatures. Its concentration reached 13.8 ± 2.7 μg mL(-1) and 16.1 ± 2.1 μg mL(-1) at 12°C and 17°C, respectively, during the first minute of exposure. Blood plasma propofol decreased rapidly to 6.8 ± 0.7 μg mL(-1) and 6.3 ± 2.2 μg mL(-1) at 12°C and 17°C respectively, during the first 10 minutes of the recovery. The half-life time of propofol was 1.5 h and 1.1 h at 12°C and 17°C, respectively. We found propofol anaesthesia in trout effective and safe. However, it caused a gradual decrease of respiratory rate, and therefore a specific anaesthesia protocol should be developed.
The presence of bacteria and parasites on the gills of pike and tench from Oświn Lake in the Seven Islands Reserve was investigated. Parallel bacteriological analyses of the water were done. The materials were sampled in May-June, July, October 1999, six years after the water level had been increased. The bacteriological and parasitological results both indicate that this body of water is eutrophic. The increase in the incidence of infection with Ergasilus sieboldi and an increase in the number of heterotrophic bacteria were observed simultaneously on the gills. Based on the parasitological and microbiological analysis of gills, the prognosis for the health of pike is unfavorable.
The effect of dietary inclusion of amaranth meal on hematology and blood biochemistry, of rainbow trout Oncorhynchus mykiss was examined. The fish (mean length 35.2 ± 0.6 cm; mean weight 524.8 ± 28.5 g) were divided into three groups: a reference group (RF) fed commercial trout pellet and two experimental groups (EF5 and EF10) fed feeds containing 5.0% and 10.0% of amaranth meal respectively. Determined indices covered: packed cell volume, red blood cell count, hemoglobin concentration, mean cell volume, mean cell hemoglobin concentration, mean hemoglobin content, inorganic phosphates, total proteins, albumins, globulins, ammonia, triacylglycerols, glucose, and the activity of creatine kinase, alkaline phosphatase and aspartate aminotransferase. Supplementation of feed with amaranth meal significantly raised levels of blood glucose, cholesterol, total protein, ammonia, creatinine and aspartate aminotransferase activity in trout blood. These results indicate that inclusion of amaranth meal in extruded diets for rainbow trout can get negative effect on liver and blood biochemistry profile
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