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Virulence and antibiotic resistance of Escherichia coli isolated from rooks

100%

Kmet V., Drugdova Z., Kmetova M., Stanko M.

Annals of Agricultural and Environmental Medicine

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2013

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tom 20

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nr 2

With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.

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Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse

88%

Gregova G., Kmetova M., Kmet V., Venglovsky J., Feher A.

Annals of Agricultural and Environmental Medicine

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2012

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tom 19

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nr 1

The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.

3

Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater

88%

Cornejova T., Venglovsky J., Gregova G., Kmetova M., Kmet V.

Annals of Agricultural and Environmental Medicine

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2015

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tom 22

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nr 3

4

Occurrence of Echinococcus multilocularis and E. granulosus seropositivity in Roma and non-Roma population of Eastern Slovakia

33%

Antolova D., Jarcuska P., Janicko M., Reiterova K., Halanova M., Cechova L., Cislakova L., Geckova A. M., Marekova M., Pella D., Siegfried L., Pastvova L., Fedacko J., Kollarova J., Kolarcik P., Bobakova D., Veselska Z., Babinska I., Drazilova S., Rosenberger J., Schreter I., Kristian P., Veseliny E., Virag L., Birkova A., Kmet'ova M., Petrasova D., Carikova K., Lovayova V., Merkovska L., Jedlickova L., Valkova I.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

5

Seroprevalence to Toxoplasma gondii in Roma and non-Roma inhabitants of Eastern Slovakia

33%

Antolova D., Jarcuska P., Janicko M., Halanova M., Cechova L., Cislakova L., Geckova A. M., Marekova M., Pella D., Siegfried L., Pastvova L., Fedacko J., Kollarova J., Kolarcik P., Bobakowa D., Veselska Z., Babinska I., Drazilova S., Rosenberger J., Schreter I., Kristian P., Veseliny E., Virag L., Birkova A., Kmet'ova M., Petrasova D., Carikova K., Lovayova V., Merkovska L., Jedlickova L., Valkova I.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

Ograniczanie wyników

Virulence and antibiotic resistance of Escherichia coli isolated from rooks

Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse

Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater

Occurrence of Echinococcus multilocularis and E. granulosus seropositivity in Roma and non-Roma population of Eastern Slovakia

Seroprevalence to Toxoplasma gondii in Roma and non-Roma inhabitants of Eastern Slovakia