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The changes of the relationship between force and deformation of pelvic and pectoral limb bones were measured in broiler chickens during the first 10 weeks of life with INSTRON 4302 apparatus. The female chickens were divided into 3 groups at 4,8 and 10 weeks of age and male chickens created one group at 8 weeks of age. With increasing age the progressive increase of the resilience and the resitance to the deformation and fracture forces of bones was observed, with higher values in the pelvic than pectoral limbs. The value of fracture force in the femur increased in female chickens from the lowest value at 4 weeks of age to the highest value at 10 weeks of age. A similar increase was observed in other limb bones of the female chickens during 10 weeks of their lives. Male chickens were characterized by higher values of resilience and fracture forces in comparison to values from female chickens at the same age. The results obtained indicate to a progressive increase in the resilience and resistance of bones in chickens during their development between 4 and 10 weeks of age with higher values in male chickens and, moreover, present a possibility of developmental estimation of skeletal remodeling and mineralization with the applied method. The implications of the observed changes to the locomotor function of bones in the developing stages of chickens have been discussed.
The study aimed at the determination of dynamic relationship between mineralisation processes and antioxidative/oxidative status during the development of osteopenia. One hundred and two healthy female Wistar rats at the age of 2 months and initial body weight of 200 g were used in the experiment. The rats were divided into control (CON, n=6), sham operated (SHO, n=48), and ovariectomised (OVX, n=48) groups. Animals from SHO (n=6) and OVX (n=6) groups were sacrificed every week during 8 weeks of the experiment in order to detect dynamic changes in examined parameters. The samples were collected weekly from day 7 to day 56. The femora were examined with the use of DXA (bone mineral density) and pQCT (area, mineral content, volumetric density of trabecular and cortical part of distal femora). The pQCT scans were performed 5 mm from distal end of the tibia. Hie determination of activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in haemolysates of erythrocytes were performed spectrophotometrically. Obtained data showed wave like changes in both enzyme activities and bone parameters and indicated the importance of the 2nd -3rd and 5th -6th week after surgery as a key moment for bone metabolism and activity of enzymatic antioxidative defence during the development of osteopenia induced by bilateral ovariectomy. The obtained results proved that alterations in activity of GSH-Px and SOD, and pQCT ahead the changes registered by DXA by 7 d.
Despite its apparent stability, bone tissue is an extremely dynamic structure, responding to diverse and multidirectional interactions by changes in its metabolism. Adipose tissue is no longer seen as a mere store of energy reserves for the organism, but is also recognized as an important endocrine organ. There are several relationships between the two types of tissue, which result from the influence of fat tissue on bone tissue. This influence is expressed through a direct influence of adipokines on bone metabolism and through indirect effects and regulatory impact of adipose tissue on other hormones associated with the metabolism of bone tissue. Osteotropic effects of adipose tissue also result from mechanical load on the skeleton. The aim of this paper was to present the mechanisms of mutual interactions between adipose tissue and bone tissue. The authors also tried to answer the question of whether obesity promotes the development of osteoporosis or whether it is a protective factor for the skeletal system.
The purpose of the study was to determine the effect of Strontium Ranelate (RS) on the mineral density and mechanical properties of the femur in male Wistar rats during experimental osteopenia induced by bilateral gonadectomy. After adaptation, the rats were randomly divided and subjected to sham-operations (SHO) (n = 10) and bilateral orchidectomy (ORX) (n = 20). ORX rats were subsequently divided into the control group (ORX/K) and experimental group treated with RS (ORX/RS), administered by gavage, at a dose of 900 mg/kg b.w. The experiment lasted 60 days and then the animals were killed, blood collected, and femora isolated. Femora were analyzed using peripheral quantitative computed tomography (pQCT) to determine volumetric bone mineral density of the cortical part of the shaft of the column (Ct.vBMD), endoosteal and periosteal circumferences, cortical thickness (Ct.Th) and Strength Strain Index (SSI). Measurements were performed on 50% of bone length. The bones were tested using the three-point bending test to estimate ultimate strength and resilience. The activity of alkaline phosphatase (ALP) and concentrations of Ca and P in the blood serum were estimated. The obtained results indicated that the use of strontium ranelate in orchidectomized rats over a period of 60 days not only inhibited the reabsorbing processes induced by a lack of gonad steroids but also accelerated processes of new bone tissue formation, which improved their mechanical parameters and ultimate strength and maximum elasticity.
The purpose of the study was to determine the effect β₂-adrenergic receptor agonist-clenbuterol on tibiae mineralization of female rats with established osteopenia. The experiments were conducted on 30 female 3-months-of-age Wistar rats with an initial body weight of about 250 g. The sham-operation - SHO (n=10) and bilateral ovariectomy - OVX (n=20) were performed. After 60 days of osteopenia induction the ovariectomized rats were divided into a group fed a standard diet (n=10) and a group that received a diet supplied with clenbuterol at a dose of 5 mg/kg. After 14 days of the experiment the tibiae was isolated and tested using a DEXA densitometer, peripheral quantitative computer tomography (pQCT) and three-point bending test. The obtained results proved that a 14 day period of clenbuterol treatment significantly increased mechanical properties content and mineral density, both planar (BMD) and volumetric (vTotBMD) of the tibiae of ovariectomized rats.
The aim of the experiments was to determine the influence of the addition of herbs to the diets on the total protein content and the activity of the digestive enzymes in broiler chickens. The experiments were carried out on 210 Ross broiler chickens (in the period from 1st to 42nd day) randomly divided into 7 groups: control and 6 experimental. The experimental chickens were fed on a diet with a 2% addition of the following: hops (gr. I), linden (gr. II), melissa (gr. III), pansy (gr. IV), peppermint (gr. V), urtica (gr. VI). At the 42nd day, 10 chickens (5 males and 5 females) were chosen from each group which had a body weight closest to the average of particular group. The body weight (g) and, after slaughter, also relative liver weight (g/100 BW) and abdominal fat content (% of BW) were examined. The percentage of breast, thigh and shank muscles were examined. The proventriculus was isolated and mucosa membrane was removed from them. After homogenization of the mucosa membrane the total proteolitic activity and total content of proteins in it were analyzed. The highest body weight was determined in chickens receiving an addition of urtica. However, the addition of linden caused a 20% reduction of body weight in males and 11% in females. Considerably lower body weight was also observed in males fed on the diet with hops. The addition of hops caused an increase of liver weight and abdominal fat content. A statistically considerable increase of the total protein content in the mucosa membrane of the proventriculus in groups fed on the diets with linden, melissa, pansy, peppermint and urtica was observed. The analysis of the proteolitical activity of the mucosa membrane indicated its considerable decrease in chickens of both sexes when the diet was enriched by the addition of linden, melissa, pansy, peppermint and in females fed on a mixture with the addition of hops and urtica.
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