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1

Production and molecular characterization of two new Citrus somatic hybrids for scion improvement

100%

Cai X., Duan Y., Fu J., Guo W.

Acta Physiologiae Plantarum

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2010

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tom 32

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nr 1

Somatic hybridization by protoplast fusion from cell suspension cultures and leaf parent has been a well-established technique holding great potential for citrus variety improvement. In this study, somatic hybrid plants were regenerated from the following two fusion combinations: ‘Murcott’ tangor (Citrus reticulata Blanco × C. sinensis (L.) Osbeck) + Hirado Buntan Pink pummelo (HBP) (C. grandis (L.) Osbeck) and ‘Bingtang’ orange (C. sinensis (L.) Osbeck) + Calamondin (C. microcarpa Bunge). Somatic hybrids were selected at an early stage based on their higher capacity for embryogenesis comparing to non-hybrid cells. Flow cytometry analysis showed that all plants from pre-selected lines of the two combinations were tetraploid. SSR analysis confirmed their hybrid nature, with nuclear DNA from both fusion parents, and absence of parental specific bands was also detected. Cytoplasmic compositions of the recovered plants were further revealed by CAPS and cpSSR analysis. The allotetraploid somatic hybrids from the ‘Murcott’ tangor + HBP combination will be applied to develop triploid seedless cultivars by interploid crossing with diploid seedy citrus cultivars, and those from ‘Bingtang’ orange + Calamondin could be valuable for Asiatic citrus canker-tolerant and ornamental citrus breeding.

2

Relationship of changes in the fatty acid compositions and fruit softening in peach (Prunus persica L. Batsch)

100%

Duan Y., Dong X., Liu B., Li P.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 03

Fatty acid compositions of peach (Prunus persica L. Batsch) mesocarp tissues from ‘Kawanakajima Hakuto’ and its firm-fleshed mutant ‘Shuangjiuhong’ were examined by gas chromatography during the developmental stages from 20 days before to 20 days after fruit ripening. Fruits were harvested at 4-day intervals from July to September. The predominant fatty acids were linoleic, palmitic and linolenic acids with 27.66–48.93 %, 23.59–31.65 %, and 12.08–28.35 % in ‘Shuangjiuhong’, and 32.64–42.79 %, 23.53–28.95 %, 16.14–39.15 % in ‘Kawanakajima Hakuto’, respectively. Saturated fatty acids (palmitic and stearic acids) remained relatively constant throughout the ripeness period. On the contrast, from 15 days before ripening, notable decline in oleic acid and increase of linoleic and linolenic acids were observed in both cultivars. In addition, from 10 days before ripening, much lower levels of oleic and linolenic acids and higher proportion of linoleic acid were observed in ‘Shuangjiuhong’ than those found in ‘Kawanakajima Hakuto’. And notably higher SFA level, lower levels of UFA and IUFA in the firm-fleshed peach were investigated during those stages. Correlation analysis showed that oleic acid and SFA had very significantly positive, whereas linolenic acid, UFA and IUFA had significantly negative correlation with fruit firmness. These results above suggest that lower levels of oleic and linolenic acids, UFA and IUFA, and higher linoleic acid and SFA content represent fruits with firmer flesh and help to retain the fruit texture.

3

Proteomic analysis of the bacterial induction of resistance to atrazine in soybean leaves

88%

Chen L., Zhu X., Wang Y., Chen L., Duan Y.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 08

Atrazine is a photosystem-II-inhibiting herbicide that interferes with photosynthetic electron transport, resulting in oxidative stress. Soybean (Glycine max (L.) Merrill) is an atrazine-sensitive crop, and its productivity is severely impacted by soils containing atrazine residues. Our previous study indicated that the bacteria Klebsiella pneumoniae strain SnebYK-induced resistance to atrazine in soybean, both before and after pasteurization. In order to study the molecular mechanisms of this induced resistance, proteins change in soybean leaves induced by SnebYK was investigated using two-dimensional gel electrophoresis. Differentially expressed proteins (relative to a non-induced control) were identified using MALDI-TOF MS. Differential expression patterns were detected in soybean leaves that had been induced by the bacterium. Analysis of relative expression levels indicated up-regulation of most of the mRNAs in these samples relative to the control. The corresponding proteins were observed to be involved primarily in physiological processes, including active oxygen removal, resistance signal transduction, and photosynthesis. This is the first study to conduct proteomic analysis of a soybean resistance response induced by bacteria. It is plausible that these differentially expressed proteins may interact to play a major role in defense and/or resistance responses.

4

Hepatic differentiation from human mesenchymal stem cells on a novel nanofiber scaffold

88%

Ghaedi M., Soleimani M., Shabani I., Duan Y., Lotfi A. S.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 1

The emerging fields of tissue engineering and biomaterials have begun to provide potential treatment options for liver failure. The goal of the present study is to investigate the ability of a poly L-lactic acid (PLLA) nanofiber scaffold to support and enhance hepatic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs). A scaffold composed of poly L-lactic acid and collagen was fabricated by the electrospinning technique. After characterizing isolated hMSCs, they were seeded onto PLLA nanofiber scaffolds and induced to differentiate into a hepatocyte lineage. The mRNA levels and protein expression of several important hepatic genes were determined using RT-PCR, immunocytochemistry and ELISA. Flow cytometry revealed that the isolated bone marrow-derived stem cells were positive for hMSC-specific markers CD73, CD44, CD105 and CD166 and negative for hematopoietic markers CD34 and CD45. The differentiation of these stem cells into adipocytes and osteoblasts demonstrated their multipotency. Scanning electron microscopy showed adherence of cells in the nanofiber scaffold during differentiation towards hepatocytes. Our results showed that expression levels of liver-specific markers such as albumin, α-fetoprotein, and cytokeratins 8 and 18 were higher in differentiated cells on the nanofibers than when cultured on plates. Importantly, liver functioning serum proteins, albumin and α-1 antitrypsin were secreted into the culture medium at higher levels by the differentiated cells on the nanofibers than on the plates, demonstrating that our nanofibrous scaffolds promoted and enhanced hepatic differentiation under our culture conditions. Our results show that the engineered PLLA nanofibrous scaffold is a conducive matrix for the differentiation of MSCs into functional hepatocyte-like cells. This represents the first step for the use of this nanofibrous scaffold for culture and differentiation of stem cells that may be employed for tissue engineering and cell-based therapy applications.

5

Silicon reduces long-term cadmium toxicities in potted garlic plants

88%

Wang Y., Hu Y., Duan Y., Feng R., Gong H.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 08

6

Predictions of select control organic pollutant removal in subsurface flow wetlands

76%

Xu D., Shi Y., Duan Y., Wang Y., You Y., Yang A., Hou Y.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 6

7

Effects of soybean–tea intercropping on soil-available nutrients and tea quality

64%

Duan Y., Shen J., Zhang X., Wen B., Ma Y., Wang Y., Fang W., Zhu X.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 08

Plant intercropping is increasing in popularity, is conducive to plant growth and development and can improve plant quality and yield. In this study, we intercropped tea (Camellia sinensis) cv. ‘Su cha zao’ and soybean (Glycine max) cv. ‘Lamar’ in a tea plantation. The chlorophyll content was higher in intercropped tea leaves than in monoculture, and the different phenotypic characteristics of intercropping and monoculture were correlated with chlorophyll and carotenoid content. Our analyses showed that soybean–tea intercropping not only alleviated cold damage, but also influenced tea plant growth. Furthermore, the soil ammonium nitrogen (N) in intercropping mode increased during soybean flowering and mature periods and was highest in the soybean flowering and podding period. Catechin levels in tea leaves significantly decreased, and the amino acid and soluble sugars increased, for intercropped compared with monoculture tea leaves. The analysis of soil fertility and tea leaf physiological indices also indicated that N fertiliser was significantly positively correlated with free amino acids in tea leaves. In conclusion, soybean–tea intercropping affected the effective N content in soil, especially ammonium N, and the formation of the main physicochemical composition of tea leaves, as well as tea taste and aroma. Thus, intercropping can sustainably improve nutrient management and increase crop yield and quality.

8

Antioxidant defense against rust infection in the leaf tissue of Malus crabapple

64%

Duan Y., Hao S., Luo R., Lu Y., Li G., Zhang J., Tian J., Yao Y.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 05

Plant tissues and cells can sense and transmit stress signals, change their morphological structures, alter protein and gene expression, and activate metabolic pathways to adapt to stressful environments. However, the internal and external resistance mechanisms related to antioxidation in local tissues or cells suffering from biotic stress remain unclear. We studied the response of Malus crabapple leaves to cedar-apple rust infection, and the results revealed that significant color changes and flavonoid compound accumulation (especially anthocyanins) occurred in the rust-infected tissue (RIT), whereas no significant color changes and only flavonol and flavanone accumulation occurred in the non-infected tissue (NIT). There was an up-regulation of expression of the key structural genes and MYBs related to anthocyanins biosynthesis in the RIT, while its expression related to flavonol and flavanone biosynthesis was up-regulated in the NIT. Moreover, the accumulation of glucose, sucrose, and sorbitol among the tested carbohydrates was successively induced at higher levels in the RIT and NIT. Importantly, rust infection increased the contents of jasmonate (JA), abscisic acid (ABA), and ethylene (ETH), and significantly up-regulated related key genes in the RIT and NIT during rust spot expansion. Spearman’s correlation and redundancy analyses indicated that ABA and ETH were potentially involved in oxidative defense responses to rust spot expansion by initiating the transcription of key genes, increasing the sugar supply, and adjusting the osmotic balance.

Ograniczanie wyników

Production and molecular characterization of two new Citrus somatic hybrids for scion improvement

Relationship of changes in the fatty acid compositions and fruit softening in peach (Prunus persica L. Batsch)

Proteomic analysis of the bacterial induction of resistance to atrazine in soybean leaves

Hepatic differentiation from human mesenchymal stem cells on a novel nanofiber scaffold

Silicon reduces long-term cadmium toxicities in potted garlic plants

Predictions of select control organic pollutant removal in subsurface flow wetlands

Effects of soybean–tea intercropping on soil-available nutrients and tea quality

Antioxidant defense against rust infection in the leaf tissue of Malus crabapple