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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Hydrodynamic performance of psammosteids: New insights from computational fluid dynamics simulations

100%
Dec M.
Acta Palaeontologica Polonica
|
2019
|
tom 64
|
nr 4
The shape of dermal armor protecting the body in the Paleozoic agnathans such as the Heterostaci has an important hydrodynamic role in providing lift or drag force generation. Here, by performing computational fluid dynamics simulations (CFD), the measurements of hydrodynamic lift/ drag force and lift or drag coefficients were taken for two psammosteids Guerichosteus and Tartuosteus with reference to the pteraspid Errivaspis. This study shows the substantially higher values of the lift coefficient and lift-to-drag ratio for the psammosteids Guerichosteus and Tartuosteus compared with Errivaspis. The tendencies in the evolution of dermal exoskeleton, especially the widening of the branchial plates of psammosteids was directed towards the increased generation of lift force to provide efficient cruising.
2

Use of oromucosally administered interferon-alpha in the prevention and treatment of animal diseases

63%
Dec M., Puchalski A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 2
175-186
Interferon-a (IFN-a) is well known as a clinically effective antiviral and antineoplastic therapeutic agent. It has also been shown to have immunoregulatory properties. IFN-a stimulates a cell-mediated innate immune response and then participates in the transition of the initial host innate response to an effective adaptive immune response. IFN-a is produced in small quantities in nasal secretions during viral infections, prompting many authors to suggest that low-dose oromucosal administration of IFN-a effectively mimics nature. Moreover, the injectable high-dose interferon therapy currently approved for various human disorders causes numerous side effects. By contrast, oromucosal administration of IFN-a is not associated with toxic effects. Another distinct advantage is ease of administration: the IFN can be dissolved in drinking water or administered by nebulization to the oral or nasal cavity. This review describes the current state of knowledge concerning orally administered IFN-a, of both human and animal origin, as a prophylactic or therapeutic agent in veterinary medicine. We present the effects of IFN-a in such animals as cattle, pigs, horses, cats, dogs and chickens, and attempt to explain its mechanism of action following oromucosal administration. It is hoped that this review of the medical literature on the use of IFN-a in animals will give practitioners a better understanding of the challenges and benefits of using this interesting cytokine in clinical practice.
3
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Regulowane agrofagi niekwarantannowe w uprawach nasiennych ziemniaka

63%
Dec M., Urbanowicz J.
Ziemniak Polski
|
2022
|
tom 32
|
nr 1
4

Conglutinin, CL-43 and CL-46 - three bovine collectins

63%
Dec M., Wernicki A.
Polish Journal of Veterinary Sciences
|
2006
|
tom 09
|
nr 4
Conglutinin, collectin-43 (CL-43) and collectin-46 (CL-46) are serum proteins characteristic for Bovidae. They belong to collectins - family of oligomeric proteins composed of trimeric subunits containing collagen-like sequences joined to C-type lectin domains. The genes encoding conglutinin, CL-43 and CL-46 are located on the bovine chromosome 28, and phylogenetic analysis indicates their common origin - from the lung surfactant protein D gene. Northern blot or immunocytochemical analysis confirm biosynthesis of bovine collectins mainly in the liver (conglutinin, CL-43) and in the thymus (CL-46). The level of conglutinin in the serum of dairy cows depends on many factors such as breeding, the season of the year, the stage of the reproductive cycle and infection. The collectins are involved in the innate immune defense. They bind to microbial surface carbohydrates inducing aggregation and, thereby, impeding infectivity. On the other hand the destruction of pathogens occurs due to stimulation of effector cells. CL-43 as well as conglutinin, binds to the collectin receptor (ClqR) localized on many types of cells identified as a surface variant of calreticulin. Conglutinin and CL-43 show antiviral activities towards influenza A virus and rotaviruses. Conglutinin also displays protective activity against bacterial infections.
5
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An electrophoretic characterization of iron-transporting proteins in Mannheimia haemolytica A1

51%
Puchalski A., Urban-Chmiel R., Dec M., Wernicki A.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 3
Iron-regulated outer membrane proteins (IROMPs) in Mannheimia haemolytica A1, which function as a receptor for complexes containing iron ions, are induced by iron deficiency in the growth environment of the bacteria. Densitometric analysis of SDS-PAGE separation showed expression of IROMPs of 71, 77, and 100 kDa in the case of bacteria grown in a medium with 2,2-dipyridyl. The electrophoregrams obtained in 2-DE separations confirmed the presence of protein fractions with these molecular weights and isoelectric points ranging from 5.4 to 6.4. The results of the study also confirmed the ability of M. haemolytica A1 proteins involved in iron uptake to induce a protective immune response. In Western blot with serum from convalescent calves naturally infected with M. haemolytica A1, distinct reactions were obtained for IROMPs of 71, 77, and 100 kDa.
6
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Krążące komórki śródbłonka jako marker uszkodzenia śródbłonka naczyń

51%
Dec M., Bartoszek E., Mosiewicz J.
Medycyna Ogólna i Nauki o Zdrowiu
|
2015
|
tom 21(50)
|
nr 2
Wprowadzenie i cel pracy. Krążące komórki śródbłonka (CECs) są to komórki, które pojawiają się we krwi obwodowej na skutek złuszczania się śródbłonka naczyń w przebiegu schorzeń układu sercowo-naczyniowego, nowotworów, zakażeń czy zapaleń. U osób zdrowych komórki te nie występują we krwi obwodowej lub ich liczba jest bardzo mała. Celem prezentowanej pracy jest podsumowanie doniesień dotyczących CECs – chorób, w przebiegu których dochodzi do wzrostu liczby tych komórek we krwi obwodowej oraz sposobów ich detekcji. Skrócony opis stanu wiedzy. CECs możemy obecnie uznać za wiarygodny, nieinwazyjny marker uszkodzenia śródbłonka naczyń. Liczba tych komórek jest podwyższona w wielu schorzeniach przebiegających z dysfunkcją śródbłonka takich jak: ostry zespół wieńcowy, zapalne choroby naczyń, zakażenia, cukrzyca, obturacyjny bezdech senny czy nowotwory. W badaniach dowiedziono również, że liczba CECs koreluje z zaostrzeniem choroby i jej rokowaniem. Przy pomocy oceny liczby CECs obserwuje się odpowiedź na terapię, także przeciwnowotworową. Do izolacji CECs stosowane są obecnie dwie metody – immunomagnetyczna oraz z wykorzystaniem cytometrii przepływowej. Podsumowanie. Określenie liczby CECs we krwi obwodowej staje się pomocne w ocenie klinicznej chorób, prognozowaniu ich przebiegu oraz w ocenie skuteczności stosowanego leczenia. Istotne wydaje się prowadzenie dalszych badań nad występowaniem CECs w różnych schorzeniach oraz ujednolicenie metod ich detekcji z krwi obwodowej.
7
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Content available

New cranial material of the acanthothoracid placoderm Palaeacanthaspis vasta from the Lower Devonian of Podolia—phylogenetic and taxonomic significance

51%
Dupret V., Szaniawski H., Dec M., Szrek P.
Acta Palaeontologica Polonica
|
2021
|
tom 66
|
nr 2
8

Determinanty sytuacji wyzywieniowej ludnosci swiata

51%
Dec M., Pawlak K., Poczta W.
Wieś i Rolnictwo
|
2008
|
nr 2
9-25
Celem opracowania jest ocena aktualnej sytuacji żywnościowej w regionach świata dotkniętych problemem niedożywienia oraz określenie roli sektora rolnego w kształtowaniu tej sytuacji. W badaniach podjęto również próbę wyznaczenia czynników determinujących stopień wyżywienia ludności wraz z ilościowym określeniem siły ich oddziaływania. W analizie wykorzystano dane Organizacji Narodów Zjednoczonych ds. Wyżywienia i Rolnictwa (FAO).
9
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Zmiany w przewodzie pokarmowym w przebiegu plamicy Henocha i Schonleina na podstawie opisu przypadku

51%
Prystupa A., Dec M., Baraniak J., Mosiewicz J.
Medycyna Ogólna i Nauki o Zdrowiu
|
2014
|
tom 20(49)
|
nr 2
10

Evaluation of the influence of transport and adaptation stress on chosen immune and oxidative parameters and occurrence of respiratory syndrome in feedlot calves

51%
Wernicki A., Urban-Chmiel R., Puchalski A., Dec M.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 1
Immune and oxidative parameters were evaluated as indicators of the influence of stress on the occurrence of respiratory syndrome in feedlot calves. The study was carried out on 60 2 and a half-month-old Simmentaler calves transported at feedlot. Mean daily feed consumption, daily weight gain, and behavioural activity were evaluated. Blood was collected on days 1, 3, 7, 14, 21, and 28 of feedlot. Serum NO ions, lipid peroxidation, acute phase proteins, IgG, and IgM were determined. The calves showed a decrease in feed consumption during the first 7 d of the feedlot. Sporadic stereotypies were noted. NO concentration showed a significant (P < 0.05) increase on days 7 and 14. Concentration of thiobarbituric acid reactive substances (TBARS) increased (P < 0.05) on days 1 and 3. IgM concentration was found to be considerably lower on days 14 and 28. Serum haptoglobin level showed a significant increase in stressed calves on days 1, 3, 7, and 14 of the leedlot. Based on these results, it can be suggested that stressors associated with transport and adaptation to the feedlot induce a stress reaction in calves, resulting in behavioural disorders, reduced weight gain, suppression of the humoral immunity and increased morbidity during the first weeks. These parameters seem to be crucial in evaluating the animals' health and welfare.
11

Characterization of Hsp70 proteins in bovine leukocytes induced by the temperature 41 degrees Celsius

45%
Urban-Chmiel R., Puchalski A., Wernicki A., Dec M., Paluch E.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 3
Heat shock proteins (Hsp) are the group of proteins observed in both prokaryotic and eukaryotic cell types. Hsp synthesis takes place in response to many environmental conditions, including ultraviolet radiation, heavy metal ions, hypoxia and toxic agents. Many authors have suggested that Hsp can be used in immunoprophylaxis, yet Hsp70 proteins expressed in bovine leukocytes have not been fully characterized. Hence the aim of this study was to evaluate the expression of Hsp70 proteins in bovine leukocytes exposed to temp. 41℃. The material for the study consisted of bovine white blood cells incubated at 41℃ for 2 hours. SDS-Page electrophoresis, Western blotting, and two-dimensional electrophoresis (2D) were performed to estimate the proteins obtained. The results of the study confirmed the influence of the temperature of 41℃ on induction of Hsp70 in bovine leukocytes. These proteins were mainly localized within molecular mass 70kDa. Some of the proteins with molecular mass from 20 to 50 kDa also showed positive reactions in Western blotting with anti-Hsp70 antibodies. Analysis of 2D electrophoresis showed a change in the localization of these proteins in the pH gradient. It can be postulated that analysis of Hsp70 expression in bovine leukocytes can be a very helpful marker for evaluating an organism’s adaptation to environmental heat stress. The proteins obtained also showed immunological reactivity with rabbit antibodies in Western blotting reactions, indicating that they can be used as protective factors in the pathogenesis of many diseases.
12
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Content available

Characterization of outer membrane proteins participating in iron transport in Pasteurella multocida serotype A3

45%
Puchalski A., Dec M., Wernicki A., Urban-Chmiel R., Gieral A.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
121-127
Iron-regulated outer membrane proteins (IROMPs) of P. multocida serotype A3, which function as receptors for complexes containing iron ions, are induced by iron deficiency in the bacterial growth environment. Analysis of an electrophoresis image of proteins isolated from bacteria grown on medium supplemented with 2,2'-dipyridyl revealed expression of 16 new proteins that were not noted in the case of the bacteria grown in standard conditions, with molecular weights from 30 to 160 kDa. Induction of IROMP expression occurred within 30 minutes after restricted iron conditions were established. In immunoblotting, distinct reactions were noted for proteins of molecular weight ranges of 25-49 kDa, 61-95 kDa, and 108-214 kDa. Proteins of the molecular weight of 68, 75 and 86 kDa were analysed using mass spectrometry and matched with the highest probability to proteins in the NCBI data base. Several dozen different proteins with similar amino acid sequences were matched to each sample.
13
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Content available

Effect of conglutinin on phagocytic activity of bovine granulocytes

45%
Dec M., Wernicki A., Puchalski A., Urban-Chmiel R., Radej S.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 3
In the present study we investigated the effect of bovine conglutinin on the phagocytic activity of leukocytes. We measured both the chemotactic activity of conglutinin and its effect on the internalization of zymosan particles and E. coli by granulocytes. We also assessed the binding of conglutinin to various microorganisms isolated from clinical cases in cattle. We showed that conglutinin binds strongly to the surface of yeast cells and to mannan-rich zymosan particles, while weak binding was observed in the case of the bacterial strains tested, including those whose O antigen is composed of mannan. Conglutinin (1-10 μg/ml) neither acts as a chemotactic factor for peripheral blood leukocytes nor affects ingestion of E. coli by granulocytes. However, as flow cytometry based assay showed, conglutinin (0.1-1 μg/ml) increased ingestion of zymosan expressed as mean fluorescence intensity (MFI) of positive cells.
14

Charakterystyka elektroforetyczna bialek blony zewnetrznej szczepow Salmonella enteritidis hodowanych w zroznicowanych warunkach oraz ocena ich wlasciwosci antygenowych

45%
Puchalski A., Kolasa A., Dec M., Urban-Chmiel R., Kowalczyk-Pecka D.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 02
193-196
The purpose of the study was both to estimate the electrophoretic profiles (SDS-PAGE) of outer membrane proteins among Salmonella Enteritidis strains isolated from humans and poultry, as well as to identify the antigens which can induce the appearance of a specific antibody response among vaccinated animals. The densitometry analysis of electrophoregrams showed that a single protein complex of 21 fractions was distinctive for strains grown in standard conditions. Among these fractions those with 20, 22, 35 and 37 kDa characterized by high optical density were found dominant. A single protein profile with 24 fractions was also characteristic of strains grown in conditions with restricted access to iron ions. A high optical density was a feature of proteins with 19, 35, 37, 55 and 74 kDa. Adding of 200 µM 2,2’-dipirydyl to the culture medium caused the expression of proteins and 78 kDa taking part in obtaining and transporting iron through S. Enteritidis within a mass range of 19, 55, 70, and 74. Densitometry analysis of electrophoregrams obtained from nitrocellulose membranes for proteins from bacterial strains grown in conditions with restricted access to iron ions indicated the presence of specific, intensive reactions towards the antigens of 35 and 55 kDa. As far as proteins obtained from bacterial culture grown with and without the addition of chelator were concerned, distinctive reactions were found towards antigens of 35 and 58 kDa. The main protein present in electrophoregrams of bacteria grown both with and without a supplement of chelator and in immunoblotting reactions was that of 35 kDa, belonging to porins of OmpA type.
15

Alternatywne metody zwalczania biegunek u cieląt w pierwszych tygodniach życia

45%
Urban-Chmiel R., Puchalski A., Dec M., Nowaczek A., Wernicki A.
Weterynaria w Terenie
|
2017
|
tom 11
|
nr 2
16

Antimicrobial properties of gold, silver, copper and platinum nanoparticles against selected microorganisms isolated from cases of mastitis in cattle

39%
Wernicki A., Puchalski A., Urban-Chmiel R., Dec M., Stegierska D., Dudzic A., Wojcik A.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 09
The study evaluated the antimicrobial properties of commercial preparations containing nanoparticles of silver, gold, copper and platinum against Escherichia coli, Streptococcus uberis, Staphylococcus aureus, Candida albicans and Candida krusei isolated from cases of mastitis in cattle. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of the preparations in relation to the isolated microbes were determined. The highest growth-inhibiting activity against the pathogens was noted in the preparations containing nanoparticles of silver and copper, whereas the preparation containing gold nanoparticles had a significantly weaker effect. Platinum nanoparticles at the concentrations applied did not exhibit biocidal activity towards the microorganisms analysed. After 30 minutes the antimicrobial activity of the silver nanoparticles, at concentrations of both 50 and 25 ppm, resulted in the complete elimination of viable cells of the microbes isolated from cases of mastitis. The preparation containing copper nanoparticles exhibited biocidal activity only at a concentration of 50 ppm.
17

Ocena wrażliwości pałeczek Lactobacillus izolowanych od kurcząt na kokcydiostatyki

39%
Dec M., Puchalski A., Wernicki A., Stepien-Pysniak D., Marek A., Urban-Chmiel R.
Postępy Mikrobiologii. Suplement
|
2019
|
tom 58
|
nr 1
18
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Detection of the bovine viral diarrhoea virus (BVDV) in young beef cattle in eastern and south-eastern regions of Poland

39%
Wernicki A., Urban-Chmiel R., Stegierska D., Adaszek L., Kalinowski M., Puchalski A., Dec M.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 1
In view of the scarcity of information concerning viral diarrhoea virus (BVDV) infections in beef cattle in Poland, the aim of this study was to evaluate the presence of the BVDV in young beef cattle from selected herds in eastern and south-eastern regions of Poland. The material consisted of 78 sera obtained from beef cattle from 15 farms, aged 6-12 months. The anti-BVDV antibody level in the sera was estimated with an ELISA kit, and detection of the BVDV was carried out by standard PCR and one step Real-Time RT-PCR. The ELISA results showed a high degree (80%) of positivity in 5 of the 78 samples. In 7 samples the degree of positivity was in the very low range: <40%. Of the 78 cDNA samples, the presence of genetic material with a length of 288 bp was found by standard PCR in 3 sera. The genetic material of BVDV was also found in the sera of the same three calves by Real-Time HRM PCR. BVDV infection in young beef cattle in south-eastern Poland is not a significant problem. This was confirmed by the positive ELISA results for 6.4% of the animals and the positive PCR results for 3.9%. The percentage of positive beef herds was about 8.6%. However, due to the severe nature of the disease and rapid transmission of the virus, regular monitoring of BVDV should be carried out.
19

Identyfikacja i lekowrażliwość pałeczek Lactobacillus izolowanych od gołębi hodowlanych

39%
Dec M., Ogorzalek P., Nowaczek A., Stepien-Pysniak D., Puchalski A., Urban-Chmiel R.
Postępy Mikrobiologii. Suplement
|
2019
|
tom 58
|
nr 1
20

Prevalence of bovine herpes virus type 1 in small herds of young beef cattle in South-Eastern Poland – a preliminary study

39%
Wernicki A., Urban-Chmiel R., Rola J., Socha W., Stegierska D., Dec M., Puchalski A.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 4
The study was performed on nasal swabs, tracheal samples, and sera obtained from young beef heifers aged between 6 and 12 months, from farms in eastern and south-eastern Poland. The samples were evaluated using bovine herpesvirus 1 (BHV-1) ELISA kits (ELISA BHV1 antibody and ELISA BHV1 antigen) and PCR. Among all the animals examined, 37 (32.2%) were positive in the ELISA BHV1 antigen test. The presence of BHV-1 was confirmed by PCR in 42 (36.5%) animals. In the ELISA BHV1 antibody test, 39 (33.9%) seropositive animals were identified. The presence of BHV-1 positive samples was observed in all the examined breeds of young cattle. There were no significant differences (P ≤ 0.05) in BHV-1 positive samples. The results indicate that the incidence of BHV-1 infections in feedlot cattle herds studied was 32.2%-36.5%, which suggests that preventive measures should be implemented in order to limit transmission of the virus.
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