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Morphological characters of spores and mycorrhizae of Glomus intraradices, as well as spores of Pacispora robiginia, arbuscular mycorrhizal fungi of the phylum Glomeromycota, were described and illustrated. Additionally, the known distribution of these species in both Poland and other regions of the world was presented. Both the species were not so far recorded in Poland and this paper is the second report of the finding of P. robiginia in the world.
Arbuscular mycorrhizal fungi (AMF) and dark septate endophyte (DSE) associations were studied in 36 medicinal plant species from 33 genera and 17 families, collected from the Botanical Garden of the Jagiellonian University in Kraków. Arbuscular mycorrhiza (AM) was found in 34 species (94%); 26 were of the Arum-type, 4 – Paris and 4 taxa revealed intermediate morpho­logy. The abundance of AMF hyphae in roots varied with particular species, ranging from 2.5% (Helianthus tuberosus) to 77.9% (Convallaria majalis). The mycelium of DSE was observed in 13 plant species (36%), however, the percentage of root colonization by these fungi was low. Spores of 7 AMF species (Glomeromycota) were isolated from trap cultures established from rhizosphere soils of the investigated plants: Archaeospora trappei (Archaeosporaceae), Glomus aureum, Glomus caledonium, Glomus claroideum, Glomus constrictum, Glomus mosseae, Glomus versiforme (Glomeraceae). Our results are the first detailed report of root endophyte associations of the plant species under study. Moreover, the mycorrhizal status of 14 plant species is reported for the first time.
The mycorrhizal status of 24 plant species considered as endemic, endangered in Poland and included in the IUCN Red List of Threatened Plants is reported. Selected plants and rhizosphere soil samples were collected in the Tatra Mts (Western Carpathians). Individuals of seriously threatened taxa were obtained from seeds and inoculated with available AM fungal strains under laboratory conditions. AM colonisation was found in 16 plants; 9 species were of the Arum-type, 4 - Paris and 3 taxa revealed intermediate morphology. The mycelium of the fine endophyte (Glomus tenue) and dark septate fungi (DSE) were observed in the material collected in the field. 20 AMF species (Glomeromycota) found in the rhizosphere of the investigated plants were reported for the first time from the Tatra Mts. The results provide information that might be useful for conservation and restoration programmes of these species. Application of AMF in active plant protection projects is discussed.
Morphological characters of spores, as well as sporocarps and spores of Ambispora gerdemannii and Glomus badium, respectively, arbuscular fungi of the phylum Glomeromycota, are described and illustrated. Additionally, the known distribution of these species in both Poland and the other regions of the world is presented. Ambispora gerdemannii was not earlier reported from Europe, and G. badium is a new fungus for Poland.
The presence of arbuscular mycorrhizal fungi (Glomeromycota, AMF) in soils may be crucial for sustainable agriculture. Although AMF impact on the performance and accumulation of therapeutic compounds of several medicinal plant species has been well documented, the investigations on the influence of medicinal plants being cultivated on AMF have been insufficiently studied. The effect of three-year monocultures of mycorrhizal (Hypericum perforatum, Levisticum officinale, Mentha × citrata subsp. citrata and Thymus vulgaris) and non-mycorrhizal (Chelidonium majus) medicinal plant species on AMF propagule abundance, species richness and composition was therefore examined. The AMF non-host plant species C. majus decreased the abundance of AMF propagules in the soil, whereas the mycorrhizal plants maintained the AMF propagule potential at the same level, however, they changed the composition of AMF species. The results showed that the choice of medicinal plant species, grown even for a relatively short period of time in a monoculture, can substantially alter the AMF potential of soils which in turn can influence the performance of other medicinal plants cultivated subsequently.
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