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A field experiment was performed during the years 2003-2005. An influence of EM (Effective Microorganisms) on the composition and number of fungi isolated from soil was found in the experiment. A total of 18111 fungal cultures were isolated from the roots of pea at flowering stage. The highest amount and the lowest diversity of fungi were obtained from rhizosphere of pea treated with pesticides (3257 colonies). The greatest diversity of fungi was found in the case of rhizosphere and rhizoplane, where EM was used in soil with the supplement of pesticides. The greatest amount and diversity of fungi were found from the plant roots of the control object. During the flowering stage, the fungi most frequently isolated from soil belonged to the genus Penicillium (40.78% of all isolates) and Fusarium (33.37% of all isolates).
Various diagnostic methods were used to evaluate the effect of fungicide protection on the prevalence of pathogenic fungi in wheat grain. Winter wheat cv. Nutka and Zyta was grown during a field experiment established in the Production and Experimental Station in Bałcyny in 2006–2007. The experimental factor was chemical crop protection: epoxiconazole, kresoxim-methyl and fenpropimorph applied at growth stages BBCH 33–35 as well as dimoxystrobin and epoxiconazole applied at BBCH 51–53. In this experiment, microscopic observations and conventional PCR assays were used as complementary methods. The quantification of Fusarium poae DNA by qPCR demonstrated the effectiveness of chemical protection against the analyzed fungal species. Lower monthly precipitation levels and higher daily temperatures intensified grain infections, in particular those caused by F. poae. A significant correlation was determined between the number of F. poae cultures isolated from winter wheat grain and the quantity of pathogenic DNA in grain identified by qPCR. Grain infections caused by F. poae lowered yield and thousand seed weight.
Field studies were carried out in the 2004 – 2005 growing seasons. The mycological analysis of malting barley (varieties Prestige and Sezam) grains was performed twice: on seeds stored for 30 days and on seeds stored for fi ve months. The infl uence of fungicide treatment on species diversity and the amount of fungal pathogens on kernels of both varieties of malting barley were determined in the studies. Main fungal pathogens of both varieties of malting barley were fi eld fungal species, such as: Alternaria alternata, Epicocum purpurascens, and fungi of the genus Fusarium. The extension of the grain storage period to fi ve months resulted in an increased share of pathogenic species.
A study into the sanitary state of roots and culm base of winter wheat was carried out in 1999-2002 in the Production and Experimental Station in Balcyny near Ostróda. Experimental wheat was cultivated after spring cross plants such as spring oilseed rape (Brassica napus ssp. oleiferus Metz.), white mustard (Sinapis alba L), Chinese mustard (Brassica juncea L.), oleiferous radish (Raphanus sativus var. oleiferus L.), false flax (Camelina sativa L.), crambe (Crambe abbysinica Hoechst.) and after oats (Avena sativa L.) as a control. The other experimental factor was the method of after-harvest residue management, i.e. ploughing in the stubble, ploughing in the stubble and straw, ploughing in the stubble and straw with nitrogen added. The occurrence of root rot and stem base diseases was affected by weather conditions and forecrop species. Winter wheat roots were attacked to the lowest degree when spring rape and radish were used as forecrops, and to the highest degree - when grown after oat. The culm base was most intensely infected with fusarium foot rot (Fusarium spp.). The remaining root-rot diseases occurred every year but with different intensity. The method of utilization of after-harvest residues did not have a clear effect on the intensity of infection of the roots and culm base of winter wheat.
Field experiments conducted during the years 2003-2005 showed that the rate of photosynthesis and transpiration decreased as a result of pea infection by Peronospora viciae. Foliar application of effective microorganisms (EM) combined with chemical control increased the rate of photosynthesis in pea, while other methods of EM application reduced net photosynthesis values (An). Chemical control and seed dressing with the tested biological agent caused a significant decrease in molar transpiration (E) values, compared to the control treatment. Soil application of EM contributed to inhibiting fungal pathogen infestation on pea plants.
Fungal communities isolated from the rhizosphere of spring cruciferous plants were analysed in the study. It was found that the rhizosphere of crucifers was colonized primarily by fungi of the order Mucorales and of the genus Fusarium. Members of the genus Fusarium dominated in the rhizoplane. The roots of cruciferous plants secrete glucosinolates – secondary metabolites known for their antifungal properties, thus affecting the communities of soil-dwelling fungi.
The present study was conducted during the years 2000-2002 at the Production-Experimental Station of the University of Warmia and Mazury in Bałcyny near Ostróda to determine the health status of the roots and stem bases of spring wheat grown after the following spring cruciferous plants: oilseed rape (Brassica napus ssp. oleiferus Metz.), Chinese mustard (Brassica juncea L.), white mustard (Sinapis alba L.), radish (Raphanus sativus var. oleiferus L.), false flax (Camelina sativa L.), Spanish colewort (Crambe abbysinica Hoechst.) and oat (Avena sativa L.) which served as control. A mycological analysis of the roots and stem bases of spring wheat was performed at the tillering stage (GS 28) and at the dough stage (GS 87) respectively. It was found that the species composition and abundance of fungal communities were affected by the forecrop and crop residue management (CRM) practices. A total of 551 fungal colonies were isolated from the roots of spring wheat. Fungi of the genus Fusarium were isolated most frequently (30.5% of all isolates). The species Fusarium equiseti, F. culmorum and F. oxysporum dominated among them. A total of 1605 fungal colonies were isolated from the stem bases of spring wheat. The highest total number of isolates was obtained from treatments which involved ploughing in stubble and straw (408 colonies). Regardless of the forecrop, members of the genus Fusarium posed the most serious threat to the stem bases of spring wheat (69.0% of all isolates). The dominant species was F. avenaceum (40.6% of all isolates), but F. culmorum (9.5%) and F. equiseti (8.4%) were also relatively abundant.
The research was conduced in the years 2000-2002. The aim of the research was to determinate the health of leaves and ears of spring wheat cultivated after spring cruciferae plants such as: spring oilseed rape (Brassica napus ssp. oleiferus Metz.), Chinese mustard (Brassica juncea L.), white mustard (Sinapis alba L.), oleiferous radish (Raphanus sativus var. oleiferus L.), false flax (Camelina sativa L.), crambe (Crambe abbysinica Hoechst.), as well as after oat (Avena sativa L.) as control. Spring wheat cv. Torka was sown after: ploughed stubble cultivated on this field, ploughed stubble and straw, ploughed stubble with straw and 30 kg nitrogen per hectare. During all the years of studies on leaves and ears of spring wheat septoria of leaf blotch and glume blotch (Mycosphaerella graminicola, Phaeosphaeria nodorum) were found. Brown rust (Puccinia recondita f. sp. tritici) was seen on leaves of wheat only during years 2001-2002. Besides on ears fusarium ear blight (Fusarium sp.) was present in 2002 and sooty mould (Cladosporium sp., Alternaria sp.) in 2001. According to health of overground parts of plants the good forecrops to spring wheat were oat, Chinese mustard, oleiferous radish. The biggest impact on presence of diseases of leaves and ears had the weather during years of studies. The use of after harvest rests didn’t have significant influence on health of leaves and ears of spring wheat.
The experiment investigated the effect of Funaben T fungicide treatment on the yield, gas exchange parameters and health status of buckwheat seeds cv. Kora. The results indicate that the applied seed dressing fungicide contributed to an increase in buckwheat seed yield. Gas exchange parameters were similar in both experimental variants. Buckwheat seeds were colonized mostly by fungi of the species Alternaria alternata, followed by fungi of the genus Fusarium. The presence of fusariotoxins was not noted in the analyzed buckwheat nutlets. Fungicide treatment had no effect on the content of the analyzed protein fractions.
A pot experiment on different methods of EM 1 application was conducted in the period 2002–2004. The study was carried out under controlled growth conditions. The experimental factor was the method of EM1 application. Before application, the biological preparation EM1 was propagated as recommended by the manufacturer (Greenland). The health status of the aboveground parts of ‘Ramrod’ pea plants was estimated at the flowering stage. The assessment of pea leaf gas exchange parameters (An, E, Gs, and Ci) was made using a LI-Cor 6400 Portable Photosynthesis System. Soil application of EM1 combined with chemical control contributed to inhibiting Fusarium wilt of pea. Foliar application of EM1 combined with chemical control increased all gas exchange rates of pea leaves. Fusarium wilt of pea insignificantly decreased all gas exchange rates of pea leaves and the number of seeds per pod. The best method of EM1 application was soil treatment combined with chemical control, which increased all yield-related morphometric parameters of pea.
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