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A novel method was proposed for identifying air quality in China. Causality analysis-based significance tests combined with different machine-learning algorithms were carried out to achieve an automated and accurate classification. To this end, the most developed 100 cities in China were selected as study areas. We analyzed meteorological factors such as temperature, humidity, precipitation, wind speed, air pressure, sunshine duration, evaporation and grand surface temperature, and the individual industrial pollutants of NO₂, SO₂, CO and O₃ by means of time series from a large amount of air monitoring data, and focused on the causality influence of the accumulative process of each pollution ingredient on PM₂.₅. In order to better clarify the formation of haze, joint regression models were established to quantify the influence degree of different factors on the cause of PM₂.₅. Different classification models, including KNN, SVM, ensemble and decision tree were trained and tested to predict air quality. An accuracy of 90.2% with the ensemble (boosted trees) classifier was obtained in this study. Results of feature selection and classification both indicated that NO₂ took an important role in the contribution of PM₂.₅ concentrations during 2015-2017 in China.
Explants of young leaves and hypocotyls of M. crystallinum were cultured on a Murashige and Skoog's (MS) medium containing auxins, cytokinins and auxin-cytokinin combinations. Callus was obtained from the medium supplemented with kinetin and 2,4-D. Callus induction was more readily obtained from hypocotyls than from leaves. Media containing zeatin (ZT), kinetin (KT), 6-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2.4-D) or a-naphthalene acetic acid (NAA) were used for bud multiplication and shoot differentiation. Shoot formation required cytokinins. The combination of cytokinins with low concentrations of auxin improved bud multiplication and shoot formation. The regenerated shoots developed roots on growth regulator free MS medium. Complete plantlets grew in the greenhouse to maturity after transfer to soil, and seeds could be collected.
Aiming at the source of underwater acoustic emission, in order to identify the enemy emission sonar source accurately. Using the digital watermarking technology and combining with the good time-frequency characteristics of fractional Fourier transform (FRFT),this paper proposes a sonar watermarking method based on fractional Fourier transform. The digital watermark embedding in the fractional Fourier transform domain and combined with the coefficient properties of the sonar signal in the fractional Fourier transform to select the appropriate watermark position. Using the different characteristics of the signals before and after embedding, an adaptive threshold was set for the watermark detection to realize the discrimination of sonar signals. The simulation results show the feasibility and has better resolution and large watermark capacity of this method, while the robustness of the watermark is better, and the detection precision is further improved
A comprehensive analysis of normalized difference vegetation index (NDVI), land-use type, and digital elevation model (DEM) data by using the geographic information system (GIS) showed that the index had increased over time in 84 prefectures that were part of the Grain for Green Project. SPOT Vegetation (Satellite Pour l’Observation de la Terre Vegetation, or satellite observation of the earth’s vegetation) data and the NDVI showed that compared to 2000, in 2015 2.05% of the arable land in the study area was no longer cultivated and that 25% of the farmland with slopes steeper than 35° and 2.68% of the moderately sloped farmland (2-35°) had been turned into forests. The arable land had been converted mainly to woodland and grassland. The interpretation of thematic mapper (TM) images showed that forest cover had increased significantly (by nearly 22%); that vegetation cover was less than 10% over 95.27% of the area; and that the area with high vegetation cover had increased significantly. Superposition analysis of TM images and DEM data showed that the intensity of soil erosion had generally decreased, with the areas under mild and pole-strength soil erosion decreasing by more than 10%. However, the Grain for Green Project has failed to check severe soil erosion so far. At present, although 60% of the study area is covered by forests, the extent of change in the degree of vegetation cover varied over time, and the spatial distribution was uneven, being higher in the east than in the west. It is therefore important to continue to strengthen governance through such projects.
Twenty days’ exposure to 50 or 100 mM NaCl in the rooting medium substantially increased fresh and dry weights of seedling shoots of the recretohalophyte Limonium sinense while 200 or 300 mM were increasingly inhibitory. KCl treatment was only slightly stimulating (50 mM) or strongly inhibitory (100–300 mM). Lesser effects on leaf area were also seen. Diameter of foliar salt glands was significantly larger than that of controls in 100 and 200 mM NaCl with the effect being reversed at higher concentrations. Gland enlargement was also observed in the presence of 100 mM KCl, while larger concentrations reduced gland size. Generally, gland diameter was larger in the presence of NaCl than in KCl. NaCl and KCl also increased gland number per leaf and secretion rate per gland. At 100 and 200 mM NaCl or KCl, Na⁺ secretion per leaf from NaCl-treated plants exceeded K⁺ secretion rate from KCl-treated plants while at 200 mM, Na⁺ secretion per gland was significantly higher for Na⁺ than for K⁺. Evidence of cell death in leaves of salt-treated plants using Evans blue staining indicates that release of cell contents through loss of membrane integrity contributed to the secretion values. We conclude that the greater tolerance of L. sinenseto to NaCl compared to KCl is linked to the more effective secretion of Na⁺ than of K⁺ and, in turn, to a greater stimulation of salt gland formation and activity and larger gland diameter.
The haze satellite map produced by NASA in 2013 showed a "Gray Great Wall" in the sky from Beijing to Hong Kong, indicating serious air pollution in China. The air pollutants and greenhouse gases can be reduced through engineering measures known as industrial gas emission and haze reduction (GGE&H reduction), as well as through green GGE&H reduction by ecosystems such as forests and wetlands that play significant roles in carbon sequestration and atmospheric environmental purification of air, soil, and water. Compared with the industrial GGE&H reduction, the green GGE&H reduction is lower in investment and operational cost, but offers more ecological benefits and therefore is more economically feasible. In this review the roles of green GGE&H reduction are summarized and its future potential is highlighted. The possible approaches to enhance the capability of green GGE&H reduction in China are discussed.
Salt in saline land is regarded as a kind of abiotic stress that limits the productivity of plants and their geographical distribution. To understand the mechanism of how shrub willow clones seedling respond to salt stress at the proteomic level, proteins extracted from seedling leaves of salt sensitive cultivar JW9-6 and salt tolerant cultivar JW2372 were tested under salt stress for the different durations, including 2, 12 and 72 h, using 2-D electrophoresis. Totally, 83 differentially expressed proteins were found using MALDI-TOF/TOF MS. These proteins were divided into 11 classes. The primary findings from this study are: (1) enhanced ROS scavenging capacity leads to increased salt tolerance for the shrub willow that protects redox homeostasis system from being damaged; (2) different measures, e.g., the inhibition of protein synthesis, protein folding and assembly, and enhancing protein proteolysis, were essential for shrub willow seedlings to respond to salt stress; (3) salt stress could affect the pathways of photosynthesis, carbohydrate metabolism, energy supply, and metabolism for amino acid and nitrogen. (4) JW2372 are more salt tolerant than that of cultivar JW9-6 due to overall performance of the above pathways.
Eoiocossus validas gen. and sp. nov., a new genus and species belonging to Palaeontinidae (Insecta, Hemiptera), is described from the Middle Jurassic of Daohugou, Inner Mongolia, China. It differs from other genera as follows: large forewing with small clavus, Sc terminating in costal margin beyond the nodus, nodal line along m₄ -cua partly, CuA₂ with two branches and A₂ developed. The ripple-like posterior margin and fresh colour pattern of Eoiocossus may be sex characteristics. The marginal membrane and ambient vein have not distinct evolutionary implications for the Palaeontinidae.
Medicago sativa L. cv. Longzhong is a nutritious forage plant in dryland regions of the Loess Plateau with strong drought tolerance and broad adaptability. To understand the adaptation mechanism of alfalfa (M. sativa L. cv. Longzhong) to drought stress, growth, and physiological parameters including levels of chlorophyll content, osmotic adjustment, reactive oxygen species (ROS), and antioxidant enzymes and antioxidants were measured under simulated levels of drought (− 0.40, − 0.80, − 1.20, − 1.60, and − 2.00 MPa). The changes in M. sativa L. cv. Longzhong were compared with those of plants of M. sativa L. cv. Longdong control (Variety I) suited to moderate rainfall areas and M. sativa L. cv. Gannong No. 3 (Variety II) suited to irrigated areas. The results showed that root–shoot ratio, the chlorophyll (a + b) and osmolytes contents, the degree of lipid peroxidation and ROS production, and the levels of antioxidative enzymes and antioxidants increased significantly with increasing drought stress, whereas plant height, aboveground biomass, chlorophyll a/b ratio, leaf water potential (Ψ₁), and relative water content (RWC) decreased in response to drought. The Longzhong variety responded early to beginning drought stress (between 0 and − 0.4 MPa) compared with the controls. Under drought stress (between − 0.4 and − 2.0 MPa), the Longzhong variety had significantly higher belowground biomass, root–shoot ratio, Ψ₁, RWC, catalase (CAT) activity and reduced glutathione content than those of Varieties I and II, but hydrogen peroxide and hydroxyl free radical (OH·) contents were significantly lower. Step regression analysis showed that OH·, CAT, malondialdehyde, superoxide anion-free radical (O₂˙⁻ ), and superoxide dismutase of Longzhong had the most marked response to drought stress. In conclusion, the stronger drought tolerance of the Longzhong variety might be due to its higher water-holding capacity, root–shoot ratio, and ability to coordinate enzymatic and non-enzymatic antioxidant systems, which coordinate the peroxidation and oxidative systems.
 Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells, and the expressed protein was analyzed using a hemagglutination assay. A preliminary in vitro study on its anti-proliferative activity was performed. The results show that the recombinant PTA with an apparent molecular mass of 29 kDa can hemagglutinate rabbit erythrocytes and this activity can be inhibited by D-mannan at a low concentration. In addition, the recombinant hemagglutinin exhibited a dose-dependent anti-proliferative activity on hepatoma cells. The results of the current study suggest that PTA and other important bioactive proteins could be produced by silkworm bioreactor for biomedicine research and application.
The sex-related spatial heterogeneity of gas exchange rates over the leaf surface under salt stress was investigated in the dioecious species, Populus cathayana Rehd. Cuttings were subjected to two salt regimes: 0 and 75 mM NaCl added to the Hoagland solution, the control and the treatment group, respectively. Measurements of gas exchange parameters were taken from over 40 sites on the surfaces of representative ‘non-stressed’ and ‘salttreated’ leaves which had the same insertion point for two sexual cuttings. Compared to the control group, the treatment group showed a significant decrease in the mean values of the following: water use efficiency (WUE), Chlorophyll a (Chl a) concentration, chlorophyll b (Chl b) concentration, concentration of carotenoids (Caro), total chlorophyll concentration (TC) in two sexes, and net photosynthesis rate (Pn), stomatal conductance (gs), and stomatal length/width ratio (SR) in females. Also, in the treatment group, females exhibited lower WUE, Pn, gs, E, Chl a, Chl b, TC, and SR than males. Comparison of contour maps showed that the net photosynthesis rate decreased gradually from apical to basal zones over the leaf surface occurred in the two sexes under natural conditions, but under salt stress, the opposite trend was found in females only. The results suggest that the heterogeneity pattern of the gas exchange parameters in response to salt stress between the two sexes is quite different due to different strategies employed by males and females to maintain the photosynthesis rate under salt stress. This heterogeneity phenomenon under salt stress may mainly be attributed to the chlorophyll pigments in males and the stomatal apertures in females.
Preferential flow is important for solute transport in soil. This study aims to investigate distribution characteristics of preferential flow paths in agricultural land and to determine the effects of preferential flow on nitrate nitrogen transport. Dye tracer experiments were conducted on two farmland plots in Changping County, Beijing. Two undisturbed soil columns (with preferential flow) and two packed soil columns (without preferential flow) were used to determine the influence of preferential flow on nitrate nitrogen transport. The results showed greater nitrate nitrogen movement with a relatively higher velocity in the undisturbed soil columns, which is on average 2.31 times of that in the packed soil column. The breakthrough time of undisturbed soil columns was 12 h with 43% reduction compared with that of the packed soil columns. The preferential transport of NO-3 in the undisturbed soil columns accounted for 43.83% of the total flux and resulted in a 97.60% accumulative leached mass for NO-3 of the total mass. These results indicated that the preferential flow with a limited total flux ratio could lead to a large proportion of NO-3 transport. Tailing phenomenon was observed and found to be a unique feature in the preferential flow’s breakthrough curve. Tailing might be caused by discrepancies between the preferential flow, matrix flow, and penetration rate during infiltration.
Hes l, a hairy and enhancer of split homolog, negatively regulates inner ear hair cell differentiation. The main objective of this study was to investigate the status of the Hes l gene in the noise-damaged cochlea in relation to the degree of noise-induced hearing loss (NIHL). Adult albino guinea pigs were exposed to white-band noise (115 dB sound pressure level). Noise exposure for either 1 or hours induced significant elevations of threshold in auditory brainstem response (ABR) compared with unexposed controls. Succinate dehydrogenase staining showed that white-band noise exposure caused significant outer hair cell losses. In addition, we found significant up-regulations of cochlear Hesl mRNA and protein expressions following acoustic trauma, and Hesl mRNA expression was positively correlated with NIHL. These findings suggest that up-regulation of Hes l expression in response to noise exposure may be one of the underlying mechanisms of NIHL.
Recretohalophytes with specialized salt-secreting structures, including salt glands and salt bladders, can secrete excess salts from plant tissues and enhance salinity tolerance of plants. However, the pathway and property of salt secretion by the salt gland has not been elucidated. In the article, Limonium bicolor Kuntze was used to investigate the pathway and characteristics of salt secretion of salt gland. Scanning electron microscope micrographs showed that each of the secretory cells had a pore in the center of the cuticle, and the rice grain-like secretions were observed above the pore. The chemical composition of secretions from secretory pores was mainly NaCl using environmental scanning electron microscope technique. Non-invasive micro-test technology was used to directly measure ion secretion rate of salt gland, and secretion rates of Na⁺ and Cl⁻ were greatly enhanced by a 200-mmol/L NaCl treatment. However, epidermal cells and stoma showed little secretion of ions. In conclusion, our results provide evidence that the salt glands of L. bicolor have four secretory pores and that NaCl is secreted through these pores of salt gland.
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