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The goal of this study was to determine the frequency of anomalies in incisor teeth depending on the horse’s age, sex, diet, diseases and floating frequency. A total of 255 horses from three Polish voivodeships, Kuyavian-Pomeranian, Greater Poland and Upper Silesia, were examined. The testing of incisor teeth was non-invasive and protocol-based. The most common defects were sharp edges of incisors in the maxilla and mandible, as well as a shortened mandible in relation to the maxilla. A relationship was proved between incisor anomalies and the horses’ age, floating frequency and bad habits. Anomalies of incisors are a common problem in horses in Poland, but they do not cause serious problems in the use of these animals.
The aim of this study was to evaluate the activity of antioxidant enzymes in horses diagnosed with recurrent airway obstruction (RAO). The study was conducted on a group of 40 horses, various breeds, divided into two groups, aged 8-15 years, including 15 males and 25 mares. Blood was collected for antioxidant analyses from 20 recurrent airway obstruction (RAO)-affected horses and 20 healthy control horses. The authors determined the activity of glutathione reductase, glutathione peroxidase and glutathione S-transferase in the blood. Average reductase activity in a group of healthy horses was 79.7 U/g, while in the group of RAO-affected horses it was 39.7 U/g. The average activity of glutathione peroxidase in the group of healthy horses was 47.8 U/g, while in the group of RAO-affected horses it was 37.8 U/g. The average activity of glutathione S-transferase in the group of healthy horses was 39.8 U/g, while in the group of RAO-affected horses it was 21.8 U/g. The resulting research indicates that oxidative stress is a consequence of chronic airway inflammation in the course of RAO in horses.
Goal: The evaluation of the brainstem function by the method of auditory brainstem evoked potentials in a reference group of healthy horses and the use of the method for diagnosis in the course of some neurological diseases of horses Materials and methods: The study was conducted on two groups of animals: nine control horses and seven horses with known neurological diseases. All patients underwent full clinical examination, otoscopic examination and detailed neurological examination. The BAER test was carried out under sedation (detomidine combined with butorphanol). Sound stimulation was performed with insertion headphones emitting the sound of a volume of 75dB, 90dB, and 105dB, 1000 pulses each, with a frequency of 11Hz. Auditory threshold examination was also conducted by increasing the intensity of sound by 10 dB in a range of 10-70 dB, and emitting 300 sound pulses for each volume level. Results: In all the BAER records, waves I, II, III, IV and V were identified on both sides. In all horses in the control group waves VI and VII were impossible to identify. There were no statistically significant differences between the sexes. In the group of healthy horses, the auditory threshold was determined at a level of 30 dB in 6 horses and 40 dB in 3 horses. In the group of horses with neurological diseases, a lesion of vestibulocochlear nerve (NC VIII) due to a head injury sustained during training was diagnosed in 3 cases, air sac infection in 2 cases, and an acquired senile deafness in 2 cases. Conclusion: Records of auditory brainstem evoked potentials in most healthy horses consist of waves I to V. The BAER technique can be used for the diagnosis of functional disorders of the CNS, especially the brainstem, midbrain and thalamus, and as an objective evaluation of hearing in horses. Summary: The technique of auditory brainstem evoked potentials in horses can be used as an additional diagnostic method in neurological diseases.
Myonecrosis after intramuscular injections is not common, but may be life-threatening. This case report describes myonecrosis following an accidental intramuscular injection of menbutone. A three-year-old bay French Trotter stallion with a history of weak appetite and colic lasting ten days was treated by a field veterinarian. During the course of treatment, he was given paraffin oil and antihelminthics orally, antispasmodics, analgesics, steroids and antibiotics intravenously, as well as choleretics intramuscularly. The horse was apathetic and weak with a “tucked up” abdomen and had grade 2 ataxia, according to Mayhew’s grading system. The patient had a swollen neck on both sides and a swelling of the right front limb and the carpal area of the left front limb. The neck edema was painful and warm. The neck area was prepared and punctured; a malodorous fluid was obtained for cytology. In the material obtained, there were anaerobic rod bacteria diagnosed as Clostridium spp. Surgical fenestration and debridement were performed. The wounds were cleaned every day. The horse was given antibiotics, nonsteroidal antinflammatory drugs, fluids and gastroprotectives. After five weeks of intensive care, the condition of the horse stabilized, and significant improvement was observed. Myonecrosis may be a rare life-threatening complication following intramuscular injections or wound contamination. To the authors’ best knowledge, this is the first published report of myonecrosis after menbutone injections in the horse.
Recurrent airway obstruction (RAO) represents a serious health problem and is traditionally classified as an allergic disease, where contact with an antigen can induce clinical airway inflammation, bronchial hyper-responsiveness and reversible airway obstruction. Previous studies have demonstrated the presence of the Th2 response in the lungs of human patients with asthma and horses with heaves. These cells are involved in the production of cytokines which regulate the synthesis of immunoglobulins. 40 horses were evaluated: 30 horses with RAO and 10 healthy animals. The expression levels of interferon-alpha 1 (IFN-α1), interferon-gamma (IFN-γ), interleukin-1β, (IL-1β), IL-2, IL-4, IL-13 and tumor necrosis factor alpha (TNF-α) were measured in the serum obtained from control and RAO-susceptible horses during crisis. In all the patients, serum cytokine levels were detected. Serum median IL-13 and IFN-γ levels were significantly higher in RAO-affected horses than in the healthy group (p < 0.001). The serum median IFN-α1, IL-1β, IL-2, IL-4, and TNF-α levels were similar in both groups. These results indicate a low variability of the levels of cytokines and a high frequency of their detection in serum samples from horses with RAO. Immune mechanisms involved in equine RAO are more complex than those defined by a simple Th1/Th2 dichotomy.
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