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1

Antybiotykooporność

100%
Jablonski A., Zebek S.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2010
|
tom 05
|
nr 4
2

Patogenne E. coli u świń - mechanizm chorobotwórczości

100%
Jablonski A., Zebek S.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2017
|
tom 12
|
nr 3
3
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Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs

81%
Jablonski A., Zebek S., Kolacz R., Pejsak Z.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
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nr 1
Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.
4

Wybrane mechanizmy oporności bakterii na chemioterapeutyki

81%
Jablonski A., Zebek S., Mokrzycka A.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 07
s.449-452,tab.,bibliogr.
A phenomenon parallel to the spread of resistant strains is the emergence of new resistance mechanisms which are difficult to control. The aim of this article is to present selected mechanisms of resistance of both Gram-positive and Gram-negative pathogens present in human and animal environment. Natural resistance of bacteria is their permanent characteristic resulting from the biology of the given micro-organism, which prevents an antibiotic from penetrating into the bacterial cell. What is more important in practice is the acquired resistance. Bacteria develop resistance by acquiring genes encoding proteins that protect them from the effects of the antibiotic. In some cases the genes arise by mutation; in others, they are acquired from other bacteria that are already resistant to the antibiotic. These genes are often found on circular DNA fragments (plasmids) which spread easily from one bacterium to another, even from one species of bacterium to another.
5

First report of Leptospira infections in red deer, roe deer, and fallow deer in Poland

61%
Zmudzki J., Jablonski A., Arent Z., Zebek S., Nowak A., Stolarek A., Parzeniecka-Jaworska M.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 3
6

Application of quantitative PCR for detection of Mycoplasma suis in blood samples

51%
Jablonski A., Borowska D., Zebek S., Kowalczyk A., Dors A., Zmudzki J., Nowak A., Pejsak Z.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 4
The aim of the study was to develop and validate a real-time PCR method, using a TaqMan probe, for quantification of Mycoplasma suis in porcine blood. No PCR signals with closely related non-haemotrophic mycoplasmas were obtained. The detection limit of PCR for plasmid combined with blood DNA was determined to be 10³/reaction (5 µL of DNA) (1.2x10⁵ target copies in 1 mL of blood). The linearity of real-time PCR (near 1) indicates its use as a quantitative method. Real-time and quantitative PCR were sensitive and specific for the detection and quantification of M. suis in the blood of animals with acute and chronic form of eperythrozoonosis. Developed quantitative PCR cannot be used to detect carrier animals with a small amount of M. suis in their blood. The validity of real-time PCR used in the studies was confirmed by the low inter- and intra-assay coefficients of variation. This fact confirms the applicability of the assay in other laboratories.
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