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Investigations were conducted to study the adsorption behavior of heavy metal Cu(II) on the mesoporous silicate SBA-15 (CONH₂-SBA-15) in aqueous medium by varying parameters such as contact time, temperature, ionic strength, and competing ions. Heavy metal adsorption was broadly independent of initial metal concentration. Competing ions in the aqueous solution had a small effect on the adsorption of Cu(II) on CONH₂-SBA-15. The adsorption data for Cu(II) fit well with the Langmuir and Redlich-Peterson models. Kinetic studies showed that the kinetic data are well described by the pseudo second-order kinetic model. Initial adsorption rate increases with an increase in temperature. The intraparticle diffusion model was the best in describing the adsorption kinetics for the Cu(II) on CONH₂-SBA-15. Thermodynamic analysis indicates that the adsorption is spontaneous and endothermic.
Winter bedding sites used by red deer Cervus elaphus xanthopygus Milne-Edwards, 1867 and roe deer Capreolus capreolus bedfordi Thomas, 1908 were studied in the forests of northeastern China from 1991 to 1992. Night beds used by both cervids were higher in canopy closure than day beds. Roe deer selected night bedding sites with denser canopy closure and higher percentage of coniferous trees, and day beds with better concealment cover than red deer. Roe deer selected night beds with higher canopy closure during cold mid winter period than during early and late winter period, whereas no such difference existed in day beds. Red deer showed no significant difference in canopy closure over day and night beds between mid and late winter. Canopy closure, composition of coniferous species, average distance to the nearest tree, and ground cover were identified to discriminate night and day beds for both cervids. The difference in selection of bed-site may reflect different energy requirements of large-bodied red deer and small-bodied roe deer.
Seasonal composition and quality of diets of red deer Cervus elaphus Linnaeus, 1758 were investigated, basing on microhistolagical analysis of composite fecal samples in the Less Xingan Mountains, northeastern China. Red deer consumed more graminoids (46%) in spring, shifted to forbs (45%) in summer, and returned to graminoids (35%) in autumn. Availability and high quality [high level of crude protein (CP), and low level of neutral detergent fiber (NDF) and acid detergent fiber (ADF) in farbs in summer] appeared to be two key factors causing these changes in diets. Winter diets were dominated by browses (74%) and the horsetails Equisetum hiernale (21%). Three forbs (Oxalix corniculata, Caltha palustris, Agrimonia pilosa) were prevalent in diets during snow-free seasons, and aspen (Populus spp.) was an important browse species in all seasons, especially in winter (32%). Forage and diet quality changed seasonally. Dietary CP and in vitro dry matter digestibility (IVDMD) declined significantly from spring to winter (from 19.6 to 6.4% and from 61.1 to 32.9%, respectively). In contrast, dietary NDF and ADF increased from 55.6 to 69.9% and from 27.9 to 54.3%, respectively. Red deer diets showed different patterns of seasonal variation in mineral contents. In most cases, dietary Ca, P and K were adequate, but Na appeared deficient all the year round. It is concluded that red deer in this region are typical mixed feeders and their diet shows seasonal changes in quality, similar to those of North American wapiti. Although red deer may have enough protein in most seasons, energy might be a key limiting factor in severe winters.
The functionalized mesoporous’ potential ability of binding heavy metals using different templates, namely cetyltrimethylammonium (CTAB) (adsorbent A) and triblock copolymer (Pluronic P123, EO20PO70EO2O, Mav = 5800) (adsorbent B), was evaluated. The synthesized materials were characterized by powder X-ray diffraction (XRD), nitrogen gas sorption, FT-IR and solid-state ¹³C and ²⁹Si NMR spectroscopy and thermogravimetry (TGA) measurements. In the adsorption experiment, the effects of several variables (stirring time, pH and presence of other metals in the medium) were studied. On the basis of these results, it was observed that the adsorption capacities were higher for adsorbent B than adsorbent A. The results also confirmed that the ureido group (CO-NH₂) has a good affect of binding metal ion.
To understand the physiological response under salt stress, photosynthesis, PSII efficiency, contents of ions and free amino acids in leaves of Caragana korshinskii Kom (Caragana) exposed to three levels of salinity were investigated. Results showed that the decrease in photosynthesis of Caragana with salt stress was largely dependent on stomatal closure during the experimental period. In the early period of stress, due to the dissipation of excess excitation energy which occurred by the increase in nonphotochemical quenching, photodamage was avoided and maximum efficiency of PSII was not affected. However, with increased salt stress, the photoprotective mechanism was not sufficient to avoid oxidative damage. Thus, damage to PSII and its resulting non-stomatal inhibition of photosynthesis may occur. At 18 days with 300 mM NaCl treatment, a non-stomatal factor was responsible for the inhibition of photosynthesis. Accumulation of Na⁺ and K⁺ in leaves indicated no competition between Na⁺ and K⁺ absorption, which suggests the potential for a unique pathway of Na⁺ absorption in Caragana. There was a critical salinity level for the accumulation of free amino acids in salt-treated leaves of Caragana, i.e., free amino acids accumulated slowly below critical level, but rapidly above the critical level. In addition, proline was the most abundant among all individual free amino acids.
Ecological industry can be thought of as a move toward sustainable development while reducing waste and avoiding pollution, especially in resource-based cities. This study presents a system dynamics approach to analyzing the environmental and economic effects of the eco-industry system by simulating long-term trends. Focusing on West China’s typical coal-based city of Huinong, a system dynamics model named industry-effect-policy system dynamics (IEP-SD) has been designed to evaluate the integrated effects of the industrial system. The simulation results indicate progressive environmental and economic benefits of the eco-industry system, along with prospective economic growth. In addition, the model identifies some defects and disadvantages of western China’s typical industry system, such as the extensive increase of local policy-preferential industrial sectors, limited and torpid development of other industries, and a certain level of industrial structure instability. An industrial structure coordination option is finally offered and then proved by the model to balance local environmental and economic effects.
We investigated the responses of growth and non-structural carbohydrates to submergence and de-submergence in three wetland macrophyte species. Survival rate, recovery ability, and soluble sugar and starch contents of flood-tolerant Polygonum hydropiper and of flood-sensitive Phalaris arundinacea and Carex argyi from Dongting Lake wetlands were investigated after 20, 40, and 60 days of complete submergence without light and 10 days after de-submergence. Plant dry weight and soluble sugar and starch contents decreased in all species during the submergence period. The decreases were slowest in P. hydropiper, intermediate in C. argyi, and most rapid in P. arundinacea. After 60 days of submergence, survival rates were 100, 50, and 0 % in P. hydropiper, C. argyi, and P. arundinacea, respectively. After recovery, plant dry weight increased in P. hydropiper and in C. argyi, but decreased in P. arundinacea. Compared to pre-submergence, soluble sugar contents generally increased and then remained relatively constant after recovery in all species, while starch content increased in P. hydropiper and decreased in P. arundinacea with increasing submergence time. For C. argyi, starch content decreased after recovery from the 20-day submergence, but increased after recovery from the 40- and 60-day submergences. These data illustrate mechanisms behind the flood tolerance of P. hydropiper and the sensitivity to flooding in P. arundinacea and C. argyi. These mechanisms include lower consumption and quicker accumulation of non-structural carbohydrates in flood-tolerant plants.
The acid pre-washing of zero valent iron for improving removal efficiency would deduce the secondary pollution in groundwater, and more fine particles of zero valent iron would reduce the permeability of aquifers. In order to better understand the approaches of nitrate removal, a series of laboratory experiments was conducted in this study. Batch tests showed that washed zero valent iron powder and activated carbon are more efficient for removing nitrate than cemarite and zeolite, similar with the acid pre-washing zero valent iron. X-ray diffraction phase analysis showed that a kind of oxide Fe₃O₄ generated on the surface of the washed iron powder particles, which is mixed with Fe₂O₃ and FeO, is relatively loose and can improve the efficiency of nitrate removal. A continuous flow column system test showed that the coarse sand-zero valent iron mix (R1), the coarse sand-zero valent iron-activated carbon mix (R4), and the coarse sand-zero valent iron-sawdust mix (R5) are more effective for reducing nitrate than the coarse sand-activated carbon mix (R2) and the coarse sand-sawdust mix (R3). Components such as NO₂-N and NH₄⁺-N would have environmental concerns as well. Further chemical analysis on the fluids from nitrate removal treatment indicates that R1 and R4 are the most effective and also environmentally friendly media for nitrate removal. This study showed that R1 and R4 media could be developed into a viable technology for the removal of nitrate in high concentration of polluted groundwater.
Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of 96 K. pneumoniae strains were isolated from sputum of respiratory tract infected patients. The isolates were performed string test, AST, ESBL virulence gene, serum bactericidal and biofilm assays. Out of 96 isolates, 39 isolates (40.6%) were identified with hypervirulent phenotypes. The number of cKP exhibiting resistance to the tested antimicrobials and ESBLs were significantly higher than that of the hvKP strains. The virulence genes of K. pneumoniae such as K1, K2, rmpA, uge, kfu and aerobactin were strongly associated with hvKP than cKP. However, no significant difference was found in FIM-1 and MrKD3 genes. ESBL producing cKP and hvKP were significantly associated with strong biofilm formation (both P < 0.05) and highly associated with bactericidal effect of serum (both P < 0.05) than cKP strains. However, neither biofilm formation nor bactericidal effect of serum was found with significant difference in between ESBL producing cKP and ESBL producing hvKP strains (both P > 0.05). Although the hvKP possess more virulence gene, but they didn’t show any significant difference between biofilm formation and bactericidal effect of serum compared with ESBL producing cKP strains.
The aim of the study was to describe the effects of acetate on hypothalamic G-protein-coupled receptor (GPR) 41 or 43, 5’-AMP-activated protein kinase (AMPK) signalling, mitogen-activated protein kinases (MAPKs) signalling and (an)orexigenic neuropeptides. Forty rabbits (Hyla, 35-day old) were randomly assigned to one of two treatment groups: intravenous injection of acetate (0.5 mg · kg−1 body weight) or vehicle (control). The acetate treatment decreased the rabbit feed intake within 5 h as compared with the control (P < 0.05). Although the acetate treatment had no effect on hypothalamic neuropeptide Y, agouti-related protein, cocaine-amphetamine-regulated transcript, GPR41, acetyl-CoA carboxylase, fatty acid synthase and carnitine palmitoyltransferase-1 mRNA levels (P > 0.05), it significantly increased the gene expression of the pro-opiomelanocortin (POMC) and GPR43 (P < 0.05). Moreover, intravenous injection of acetate did not affect the protein levels of phosphorylated extracellular signal-regulated kinases, AMPK or p38 MAPK in comparison with the control group (P > 0.05); however, there was a significant increase in GPR43 protein level and decrease in phosphorylated c-Jun N-terminal kinases (JNK) level (P < 0.05). So, acetate induced anorexia via the up-regulation of hypothalamic POMC gene expression, which may be associated with membrane GPR43 and intracellular JNK signalling.
The objective of this study was to evaluate nutrient loss under herbaceous cover in simulated field conditions and quantify the influences of the aerial and underground parts of plants in preventing nutrient loss. The experimental settings were as follows: two herbs, i.e., alfalfa (Medicago sativa L.) and green foxtail (Setariaviridis (L.) Beauv.); four covers for each herb (0, 20-30%, 50-60%, and 80-90%); and two treatments (reserving the entire plant and trimming the aerial part of the plant). Through simulated rainfall, runoff and sediment samples were collected at the end of the soil tank and analysed for nitrogen (N) and phosphorus (P). The results indicated that nutrient loss gradually decreased with the increasing plant coverage, and the nutrient loss on the green foxtail slope was lower than that on the alfalfa slope under the same coverage. Although the effect of the alfalfa canopy on nutrient loss prevention was higher than that of the green foxtail, the capacity of the green foxtail root for conserving nutrients is stronger than that of alfalfa due to a different herbaceous plant leaf area index and root morphology distribution. An exponential function was found to characterize the different plant root length densities and nutrient losses on the slope.
The prolactin receptor (PRLR) gene was studied as a candidate gene for the high prolificacy of Jining Grey goats. Polymorphisms in intron 1 and intron 2 of PRLR gene were detected in high prolificacy (Jining Grey) and low prolificacy (Boer, Wendeng dairy, Liaoning Cashmere and Beijing) native goats using PCR-SSCP. For intron 1, five genotypes (AA, AH, AK, HH and HK) were identified in Jining Grey goats, and two (AA and AK) in the other four breeds. The Jining Grey does of genotype HH, HK, AH and AK delivered by 0.65, 0.62, 0.59 and 0.57 more kids (P<0.01) than those of genotype AA, respectively. For intron 2, three genotypes (CC, CD and DD) were detected in Boer goats, and two (CC and CD) in the other four breeds. The Jining Grey does of genotype CD delivered by 0.55 (P<0.01) more kids than those of genotype CC.
ASYMMERTIC LEAVES2-LIKE38 (ASL38/ LBD41), isolated from Arabidopsis, is a member of the LATERAL ORGAN BOUNDARY DOMAIN gene family. We reported that ASL38 might be involved in the dorsoventral determination in extremely folded or crinkly leaves of 35S:ASL38.cockscomb plants; suggesting ASL38 is a transcript factor, and regulates a number of genes that are involved in the morphogenesis and development of plants. To verify this speculation, in this work, we constructed the binary vector pBI121–pMD-18T, which contained the GFP and coding sequences of ASL38, and introduced them into cockscomb via Agrobacterium tumefaciens. We found that ASL38-GFP fusion protein was localized in discrete subnuclear bodies, indicating ASL38 might be a nuclear protein and function as a transcription factor. In modification of flowering plants, many potentially useful genes that are involved in the pathways associated with flower and plant morphology have been cloned. Transcription factors regulating plant development and biosynthetic or regulatory genes involved in plant hormones are common candidates. If 35S:ASL38 cockscomb plants are altered in morphology, these morphological modifications could pave the foundation for the selection of novel flower varieties. As it was speculated by us, in this work, we showed that these 35S:ASL38 cockscomb flowered earlier and their flowers were turned into multiple column patterns, when compared with wild-type cockscomb. Moreover, leaves of some 35S:ASL38 plants revealed lobed and dissected patterns, and extremely, two leaf blades were developed on the same petiole; which was never found in wild-type cockscomb. Together, these morphological modifications of cockscomb indicate that we have successfully attained some novel lines of cockscombs. These lines can have potential practical applications.
LHX4 is a LIM homeodomain transcription factor involved in pituitary ontogenesis. Some mutations of LHX4 are associated with combined pituitary hormone deficiency that affects growth and development in animal. The objective of this study is revealing to these polymorphisms of in LHX4 gene exons 1, 2, 3 in 820 Chinese cattle and to assess possible association of polymorphisms in LHX4 gene with growth traits in NY breed. The PCR-SSCP and DNA sequencing of LHX4 gene showed five novel synonymous mutations: (GenBank: NW_001493442.2: g.35143G >A, g.35152C>T, g.35212C>T, g.35230G>A, g.35233T>C). Genotype MM (GG-CC-CC-GG-TT) and haplotype M (G-C-C-G-T) were dominant in the four breeds, and genotype frequencies of LHX4 in the four cattle populations agreed with Hardy-Weinberg equilibrium (P>0.05), although their frequencies significantly differed among the four analyzed populations (***P<0.001). The association analysis showed that individuals with genotype MM had greater body weight than those with genotype MN (*P<0.05) at eighteen months of age in Nanyang cattle.
Background: Radiotherapy is among the commonly applied treatment options for glioma, which is one of the most common types of primary brain tumor. To evaluate the effect of radiotherapy noninvasively, it is vital for oncologists to monitor the effects of X-ray irradiation on glioma cells. Preliminary research had showed that PKC-ι expression correlates with tumor cell apoptosis induced by X-ray irradiation. It is also believed that the lactate-to-creatine (Lac/Cr) ratio can be used as a biomarker to evaluate apoptosis in glioma cells after X-ray irradiation. In this study, we evaluated the relationships between the Lac/Cr ratio, apoptotic rate, and protein kinase C iota (PKC-ι) expression in glioma cells. Methods: Cells of the glioma cell lines C6 and U251 were randomly divided into 4 groups, with every group exposed to X-ray irradiation at 0, 1, 5, 10 and 15 Gy. Single cell gel electrophoresis (SCGE) was conducted to evaluate the DNA damage. Flow cytometry was performed to measure the cell cycle blockage and apoptotic rates. Western blot analysis was used to detect the phosphorylated PKC-ι (p-PKC-ι) level. 1 H NMR spectroscopy was employed to determine the Lac/Cr ratio. Results: The DNA damage increased in a radiation dose-dependent manner (p < 0.05). With the increase in X-ray irradiation, the apoptotic rate also increased (C6, p < 0.01; U251, p < 0.05), and the p-PKC-ι level decreased (C6, p < 0.01; U251, p < 0.05). The p-PKC-ι level negatively correlated with apoptosis, whereas the Lac/Cr ratio positively correlated with the p-PKC-ι level. Conclusion: The Lac/Cr ratio decreases with an increase in X-ray irradiation and thus can be used as a biomarker to reflect the effects of X-ray irradiation in glioma cells.
Grass pea (Lathyrus sativus L.) is the most drought-tolerant legume crop rich in dietary protein. However, little is known about the molecular mechanisms of its drought resistance. Two-imensional gel electrophoresis (2-DE) is an important experiment technique in proteomics, which has been applied extensively in studies on plant resistance to abiotic stress. To establish an effective 2-DE platform and further study the droughtresistance mechanisms of grass pea using proteomic approaches, three protein extraction methods, different isoelectric focusing (IEF) conditions and various types of gel strips were evaluated using mature leaves. The results showed that the trichloroacetic acid (TCA)/acetone protein extraction method, extending time at low voltage for IEF and using 18 cm gel strip with pH 4.0–7.0 were optimum conditions for 2-DE analysis of grass pea leaves. Applying these optimized 2-DE conditions, 1,481 total protein spots were detected in control leaves and 1,346 spots in polyethylene glycol -treated leaves, of which 67 differentially expressed protein spots were obtained relative to the control. These data suggested that an efficient 2-DE platform with high repeatability and resolution for grass pea mature leaves had been established for the first time here, which could be further used to investigate the droughtresistance molecular mechanisms of grass pea.
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