Wyniki wyszukiwania

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Malina moroszka Rubus chamaemorus L. - wystepowanie i proba zachowania ginacego gatunku w kulturach in vitro

100%

Thiem B.

Chrońmy Przyrodę Ojczystą

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2001

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tom 57

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nr 4

16-24

2

Ocena jakosci roslin leczniczych w kulturach in vitro

100%

Thiem B.

Herba Polonica

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2007

|

tom 53

|

nr 2

31-32

3

Micropropagation of cloudberry [Rubus chamaemorus L.] by initiation of axillary shoots

100%

Thiem B.

Acta Societatis Botanicorum Poloniae

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2001

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tom 70

|

nr 1

4

In vitro culture techniques in conservation of Rubus chamaemorus L.

100%

Thiem B.

Acta Biologica Cracoviensia. Series Botanica

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2002

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tom 44

The study evaluated two methods of conserving Rubus chamaemorus L. shoot cultures: medium-cold storage and encapsulation of shoot buds. Cold-stored explants were transferred every 12 weeks to proliferation medium and their multiplication rate was observed after the first passage. Shoot cultures stored at 4°C under low light intensities for 12 months without intervening subculture survived with 90% viability. Axillary buds obtained from in vitro shoot culture of cloudberry were encapsulated in calcium alginate hydrogel. Regrowth response of encapsulated buds was estimated. Encapsulated buds stored at low temperature in the dark survived for up to 3 months without loss of viability. The fidelity of subcultured plantlets was also evaluated by phytochemical analysis (fingerprinting) of some phenolic compounds and morphological observations. Preliminary trials in the botanical garden showed that plantlets of Rubus chamaemorus derived in vitro can be used for ex situ germplasm conservation.

5

Rubus chamaemorus L. - a boreal plant rich in biologically active metabolites: a review

100%

Thiem B.

Biological Letters

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2003

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tom 40

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nr 1

6

Quality assessment of medicinal plants in vitro cultures

100%

Thiem B.

Herba Polonica

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2007

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tom 53

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nr 2

7

Micropropagation of rare and protected species - Eryngium maritimem L.

63%

Kikowska M., Thiem B.

Acta Societatis Botanicorum Poloniae

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2010

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tom 79

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nr Suppl.1

8

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Enhanced isoflavones accumulation in methyl jasmonate-treated in vitro cultures of kudzu [Pueraria lobata Ohwi]

63%

Thiem B., Krawczyk A.

Herba Polonica

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2010

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tom 56

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nr 1

48-56

Kudzu, Pueraria lobata (Willd.) Ohwi (Fabaceae) is a woody climber plant that naturally grows in tropical and subtropical East Asia, where the species is highly valued for its medicinal properties. Methyl jasmonate (MJ) stimulated isoflavones accumulation in Pueraria lobata whole plant cultures in vitro after 7 days of elicitation. Isoflavonoid content increased by the addition of 50, 100 and 200 μM of MJ. However, the plantlets growth was inhibited by increasing of MJ concentration. The elicitor (100 μM) enhanced total isoflavones production in plantlets of P. lobata to 10.44 mg/g dry wt. Moreover, MJ stimulated accumulation of daidzein and genistein. It was found that the total content of isoflavones increased in comparison with that found in the control plantlets, while content of genistein was 4–fold higher than control plants but the content of daidzein increased from undetectable level up to 0.54 mg.g-1 dry mass. The study shows that the content of active compounds in whole plant cultures of P. lobata may be enhanced by elicitation with MJ.

9

Eryngium campestre L. [mikolajek polny] i inne gatunki Eryngium L. - malo znane rosliny lecznicze

63%

Thiem B., Wiatrowska I.

Herba Polonica

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2007

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tom 53

|

nr 1

93-102

Gatunki z rodzaju Eryngium L. (Apiaceae) dostarczają surowców leczniczych stosowanych w wielu schorzeniach w medycynie ludowej i homeopatii. Autorki dokonały przeglądu występowania wybranych gatunków, obecności metabolitów wtórnych i właściwości leczniczych roślin z rodzaju Eryngium.

10

Characteristic of the cell suspension culture of Eryngium planum L.

63%

Kikowska M., Thiem B.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2009

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tom 51

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nr 1

11

Mikrorozmnazanie Rubus chamaemorus L. i wstepna analiza wtornych metabolitow

63%

Thiem B., Romaniuk S.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

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1997

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tom 50

575-576

12

The effect of cytokinins on in vitro morphogenesis of Passiflora caerulea L.

63%

Ozarowski M., Thiem B.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2009

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tom 51

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nr 1

13

Metabolity stresowe rośliny wyższych

63%

Skrzypczak L., Thiem B.

Wiadomości Botaniczne

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1987

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tom 31

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nr 3

14

Alginate-encapsulated shoot tips and nodal segments in mictopropagation of medicinal plands. A review

63%

Kikowska M., Thiem B.

Herba Polonica

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2011

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tom 57

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nr 4

In recent years, the alginate-encapsulated in vitro-derived shoot tips and nodal segments have been employed as an alternative to somatic embryos method of artificial seed production. Encapsulation of non-embryogenic propagules offers an efficient technique for clonal propagation of elite genotypes e.g. plants of medicinal importance and also enables the establishment of basal collection for gene banks. The artificial seeds technology based on vegetative micropropagules may be useful not only in large-scale propagation but also in short- and long-term conservation and germplasm exchange of desirable species genotypes. This paper presents a brief overview of current status of plants of medicinal value propagated by alginate-encapsulated non-embryogenic plant material.

15

In vitro cultures of Eryngium alpinum L. – an endangered and protected species as a source of plantlets and biomass rich in bioactive compounds

63%

Kikowska M., Thiem B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

16

Twinflower (Linnaea borealis L.) - plant species of potential medicinal properties

63%

Thiem B., Buk-Berge E.

Herba Polonica

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2017

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tom 63

|

nr 3

Twinflower (Linnaea borealis L.) is a widespread circumboreal plant species belonging to Linnaeaceae family (previously Caprifoliaceae). L. borealis commonly grows in taiga and tundra. In some countries in Europe, including Poland, twinflower is protected as a glacial relict. Chemical composition of this species is not well known, however in folk medicine of Scandinavian countries, L. borealis has a long tradition as a cure for skin diseases and rheumatism. It is suggested that twinflower has potential medicinal properties. The new study on lead secondary metabolites responsible for biological activity are necessary. This short review summarizes very sparse knowledge on twinflower: its biology, distribution, conservation status, chemical constituents, and describes the role of this plant in folk tradition of Scandinavian countries.

17

Ellagic acid in in vitro cultures of Rubus chamaemorus L.

63%

Thiem B., Krawczyk A.

Herba Polonica

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2003

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tom 49

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nr 3-4

This paper describes analysis of free and total (also liberated by hydrolysis) ellagic acid (EA) content in in vitro cultured Rubus chamaemorus and in leaves of intact plants as a control. The content of EA was determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of free EA in the shoot cultures was only 2.6 X less than in intact leaves and that callus tissue is also able to synthetize small amounts of EA.

18

Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

63%

Thiem B., Kalinowski A.

Acta Societatis Botanicorum Poloniae

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2002

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tom 71

|

nr 4

19

Wplyw pelzakostatycznych ekstraktow roslinnych na przebieg encystacji pelzakow wolno zyjacych Acanthamoeba castellani

51%

Wandurska-Nowak E., Derda M., Thiem B., Wojt W.

Wiadomości Parazytologiczne. Suplement

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2007

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tom 53

127

20

Bioactive secondary metabolites in in vitro cultures of Eryngium alpinum L.

51%

Thiem B., Kikowska M., Paluch I.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2009

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tom 51

|

nr 1

Ograniczanie wyników

Malina moroszka Rubus chamaemorus L. - wystepowanie i proba zachowania ginacego gatunku w kulturach in vitro

Ocena jakosci roslin leczniczych w kulturach in vitro

Micropropagation of cloudberry [Rubus chamaemorus L.] by initiation of axillary shoots

In vitro culture techniques in conservation of Rubus chamaemorus L.

Rubus chamaemorus L. - a boreal plant rich in biologically active metabolites: a review

Quality assessment of medicinal plants in vitro cultures

Micropropagation of rare and protected species - Eryngium maritimem L.

Enhanced isoflavones accumulation in methyl jasmonate-treated in vitro cultures of kudzu [Pueraria lobata Ohwi]

Eryngium campestre L. [mikolajek polny] i inne gatunki Eryngium L. - malo znane rosliny lecznicze

Characteristic of the cell suspension culture of Eryngium planum L.

Mikrorozmnazanie Rubus chamaemorus L. i wstepna analiza wtornych metabolitow

The effect of cytokinins on in vitro morphogenesis of Passiflora caerulea L.

Metabolity stresowe rośliny wyższych

Alginate-encapsulated shoot tips and nodal segments in mictopropagation of medicinal plands. A review

In vitro cultures of Eryngium alpinum L. – an endangered and protected species as a source of plantlets and biomass rich in bioactive compounds

Twinflower (Linnaea borealis L.) - plant species of potential medicinal properties

Ellagic acid in in vitro cultures of Rubus chamaemorus L.

Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

Wplyw pelzakostatycznych ekstraktow roslinnych na przebieg encystacji pelzakow wolno zyjacych Acanthamoeba castellani

Bioactive secondary metabolites in in vitro cultures of Eryngium alpinum L.