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1
Content available remote

Modulation of CCK-8-evoked intracellular Ca2+ waves by hydrogen peroxide in mouse pancreatic acinar cells

100%
Granados M. P., Salido G. M., Pariente J. A., Gonzalez A.
Journal of Physiology and Pharmacology
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2007
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tom 58
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nr 3
In the present study we have employed single cell imaging analysis to monitor the propagation of cholecystokinin-evoked Ca2+ waves in mouse pancreatic acinar cells. Stimulation of cells with 1 nM CCK-8 led to an initial Ca2+ release at the luminal cell pole and subsequent spreading of the Ca2+ signal towards the basolateral membrane in the form of a Ca2+ wave. Inhibition of sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) activity by 1 µM thapsigargin, preincubation in the presence of 100 µM H2O2 or inhibition of PKC with either 5 µM Ro31-8220 or 3 µM GF-109203-X all led to a faster propagation of CCK-8-induced Ca2+ signals. The propagation of CCK-8-evoked Ca2+ signals was slowed down by activation of PKC with 1 µM PMA, and preincubation of cells in the presence of H2O2 counteracted the effect of PKC inhibition. The protonophore FCCP (100 nM) and the inhibitor of the mitochondrial Ca2+-uniporter Ru360 (10 µM) led to an increase in the propagation rate of CCK-8-evoked Ca2+ waves. Finally, depolymerisation of actin cytoskeleton with cytochalasin D (10 µM) led to a faster propagation of CCK-8-evoked Ca2+ signals. Stabilization of actin cytoskeleton with jasplakinolide (10 µM) did not induce significant changes on CCK-8-evoked Ca2+ waves. Preincubation of cells in the presence of H2O2 counteracted the effect of cytochalasin D on CCK-8-evoked Ca2+ wave propagation. Our results suggest that spreading of cytosolic Ca2+ waves evoked by CCK-8 can be modulated by low levels of oxidants acting on multiple Ca2+-handling mechanisms.
2

Expression of Betta, Betta-carotene15,15 oxygenase in bovines

88%
Morales A., Gonzalez A., Shimada A., Varela A., Mora O.
Journal of Animal and Feed Sciences. Supplement
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2004
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tom 13
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nr 1
3

In vitro differentation of preadipocytes from bovine adipose tissue

76%
Grageola O., Gonzalez A., Shimada A., Castro ML F., Varela A., Mora O.
Journal of Animal and Feed Sciences. Supplement
|
2004
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tom 13
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nr 1
4
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Differential anti-insect activity of natural products isolated from Dodonaea viscosa Jacq. (Sapindaceae)

64%
Diaz M., Diaz C. E., Alvares R. G., Gonzalez A., Castillo L., Gonzalez-Coloma A., Seoane G., Rossini C.
Journal of Plant Protection Research
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2015
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tom 55
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nr 2
Botanical biopesticides constitute an important tool for Integrated Pest Management practices. Uruguay has a great potential for developing botanical biopesticides from its abundant native flora. Dodonaea viscosa Jacq. is a native Sapindaceae that in preliminary studies was shown to possess a potential deterrent activity against insect models. In this work, ethanolic extracts of the leaves of this species were studied. Bioguided fractionation and supercritical fluid extraction led to the isolation of active compounds. For that purpose four insect models were used: Epilachna paenulata, Spodoptera littoralis, Myzus persicae, and Rhopalosiphum padi, which are pests of crops of economic importance. Lupeol, stigmasterol, stigmast-7-en-3-ol, and a labdane diterpene were isolated and showed differential activity against the models.
5

Influence of different levels of dried citrus pulp on in vitro ruminal fermentation kinetics of total mixed ration in goat rumen inocula

64%
Hernandez J., Rojo R., Salem A. Z. M., Mirzaei F., Gonzalez A., Vazquez J. F., Montanez O. D., Lucero F. A.
Journal of Animal and Feed Sciences
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2012
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tom 21
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nr 3
Inclusion of dried citrus pulp (DCP) at different levels: 0 (control), 10% (DCP10), 20 (DCP20), and 30% (DCP30) of the total mixed ration (TMR) was evaluated by in vitro gas production (GP), and ruminal fermentation patterns, in a completely randomized design. Rumen fluid was collected before the morning meal from 8 gestating goats (Boer×Saanen, body weight 3±2.3 kg). GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48, 72, and 96 h of incubation. Ruminal fermentation parameters such as 96 h partitioning factor (PF96), in vitro organic matter digestibility (IVOMD), metabolizable energy (ME), short-chain fatty acids (SCFA), and metabolizable energy (ME) were also estimated. Inclusion of DCP linearly increased (P<0.01) cumulative gas production at 24, 48, and 96 h after inoculation, as well as asymptotic gas production (b). Rations at 30% and 10% DCP had the highest values of gas production, whereas control and 20% DCP presented the same values (quadratic effect P<0.01). Lag phase (L) decreased linearly (P<0.001) with DCP addition. IVDMD and IVOMD increased linearly (P<0.01) with the inclusion of DCP in rations. The ration at 30% DCP (DCP30) had the greatest value of ME and SCFA, but no differences were observed in the PF96 among treatments. The data suggest that the DCP30 ration had the best impact on rumen gas production and IVDMD, IVOMD, ME, and SCFA.
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