Wyniki wyszukiwania

1

Changes in the proteome of Pseudomonas putida KT2440 during polyhydroxyalkanaotes synthesis

100%

Mozejko-Ciesielska J., Mostek A., Szacherska K.

Postępy Mikrobiologii. Suplement

|

2017

|

tom 56

|

nr 3

2

Formation and stability of polysomes and polysomal populations in roots of germinating seeds of soybean (Glycine max L.) under cold, osmotic and combined cold and osmotic stress conditions

88%

Swigonska S., Badowiec A., Mostek A., Krol A., Weidner S.

Acta Physiologiae Plantarum

|

2014

|

tom 36

|

nr 03

Abiotic stress factors such as extreme temperatures or osmotic stress are among the major causes of inferior crop yields. In response to a stress, plants have evolved various defense mechanisms. In our study, we have demonstrated how cold stress, osmotic stress and a combination of both stresses retard the growth of roots and inhibit the process of ribosomes binding into polysomes. The tested stresses also limited the ability of root tissues to synthesize proteins. At the same time, most of the analyzed samples were found to contain elevated shares of the fractions of cytoskeleton-bound polysomes (CBP, CMBP) in the total population of polysomes. Using a polysome-based degradation system, it was shown that polysomes formed under stress conditions were much more resistant to the effect of exogenous ribonuclease than the control ones. The highest tolerance to digestion was demonstrated by the cytoskeleton-bound (CBP) and cytoskeleton-membrane bound polysomes (CMBP). The increasing share of CBP and their stability in roots of seeds germinating under stress conditions can be a target for physiological regulation. It seems that modifications in the stability and percentages of particular polysomal populations play an important role in the adaptation of plants to stress conditions, which may indicate that these forms of polysomes, i.e., cytoskeleton-bound ones, are involved (via selective translation) in the synthesis of stress proteins in soybean roots.

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Influence of abiotic stress during soybean germination followed by recovery on the phenolic compounds of radicles and their antioxidant capacity

76%

Swigonska S., Amarowicz R., Krol A., Mostek A., Badowiec A., Weidner S.

Acta Societatis Botanicorum Poloniae

|

2014

|

tom 83

|

nr 3

Abiotic stress factors are among the major causes of lower crop yields. It is known, that in response to cold and/or osmotic stress, crops activate various defense mechanisms, including morphological, physiological and metabolic adaptations. Secondary metabolism, especially phenolic compounds, seem to be an important factor of stress-induced metabolic re-engineering as their levels are alternated by abiotic stress in plants. Despite the fact, that the nature and function of phenolic compounds was already studied in various plant species, it is important to define tissue-specific changes induced by two most potent abiotic stressors – low temperature and decreased water potential. Moreover, in fields, the appearance of single stress is rather rare. Usually two or more factors are acting in parallel, which may potentially result in different effects. Therefore, the aim of this study was to analyze selected elements of secondary metabolism in roots of germinating soybean seeds under cold stress, osmotic stress and both stresses combined. In addition the effects of constant and persistent stress were compared to those induced by sudden and brief stress appearance, as well as after the post-stress recovery process. In the presented study standard methods for identification and quantification of phenolic acids and isoflavones were used and the antioxidant capacity of the radicle extracts was measured. The phenolic metabolism in plants was greatly intensified in response to cold and osmotic stress and remained at high level during the post-stress recovery. The amount and composition of both phenolic acids and identified isoflavones also changed in stress- and duration-dependent manner. This proves an important role of phenolic compounds in abiotic stress response of germinating soybean seeds and opens up new perspectives for further investigations.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Acta Societatis Botanicorum Poloniae

1 Postępy Mikrobiologii. Suplement

3 Mostek A.

2 Badowiec A.

2 Krol A.

2 Swigonska S.

2 Weidner S.

1 Amarowicz R.

1 Mozejko-Ciesielska J.

1 Szacherska K.

1 2017

2 2014

Changes in the proteome of Pseudomonas putida KT2440 during polyhydroxyalkanaotes synthesis

Formation and stability of polysomes and polysomal populations in roots of germinating seeds of soybean (Glycine max L.) under cold, osmotic and combined cold and osmotic stress conditions

Influence of abiotic stress during soybean germination followed by recovery on the phenolic compounds of radicles and their antioxidant capacity