Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Wpływ litu i zaburzeń równowagi kwasowo-zasadowej na przemiany i transport metabolitów cyklu Krebsa w nerkach

100%

Stepinski J.

Diagnostyka Laboratoryjna

|

1983

|

tom 19

|

nr 5-6

2

Wazniejsze obiekty historyczne w Nadwarcianskim Parku Krajobrazowym

100%

Stepinski J.

Biuletyn Parków Krajobrazowych Wielkopolski

|

1999

|

tom 05

170-172

3

W obronie Warty - lokalna inicjatywa samorzadowa

88%

Stepinski J.

Przegląd Przyrodniczy

|

2002

|

tom 13

|

nr 4

129-36

4

Regulation of aconitate hydratase activity from rat kidney cortex by bicarbonate

63%

Stepinski J., Angielski S.

Acta Biochimica Polonica

|

1976

|

tom 23

|

nr 2-3

5

Radioimmunologiczna metoda oznaczania adenozyny

51%

Stepinski J., Skowronski R., Wysocka D., Angielski S.

Diagnostyka Laboratoryjna

|

1985

|

tom 21

|

nr 6

6

Effect of lithium on renal gluconeogenesis

51%

Stepinski J., Pawlowska D., Angielski S.

Acta Biochimica Polonica

|

1984

|

tom 31

|

nr 2

7

Renal function in acetate treated rats

51%

Piec G., Wojnowski L., Stepinski J., Angielski S.

Acta Physiologica Polonica

|

1988

|

tom 39

|

nr 1

8

Synthesis of nucleosides tri- and tetraphosphates resistant to hydrolysis

51%

Jemielity J., Kalek M., Stepinski J., Darzynkiewicz E.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr Supplement 1

9

Inhibition of alfa-oxoglutarate and pyruvate oxidation by alfa-oxoderivatives of leucine and valine in rat tissues

51%

Lysiak W., Stepinski J., Angielski S.

Acta Biochimica Polonica

|

1970

|

tom 17

|

nr 2

10

Badania oddziaływania pomiędzy m7GMP a modelowym peptydem Trp-Len-Glu metodą wygaszania fluorescencji

51%

Wieczorek Z., Stepinski J., Darzynkiewicz E., Loanberg H.

Current Topics in Biophysics. Supplement

|

1992

|

tom 16

11

Effect of dipyridamole and furosemide on rental function during adenine dinucleotide infusion in rats

51%

Redlak M., Szczepanska-Konkel M., Stepinski J., Angielski S.

Acta Physiologica Polonica

|

1986

|

tom 37

|

nr 1

12

Type 2 (AT2) angiotensin II receptor modulates guanylyl cyclase activities in rat podocytes

51%

Golos M., Lewko B., Latawiec E., Stepinski J.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr Supplement 1

13

Zwiększenie przez sulfonylomocznik, SPC-703, powinowactwa insuliny do jej receptorów w kanalikach nerkowych szczura

51%

Stepinski J., Madrala A., Angielski S.

Diagnostyka Laboratoryjna

|

1979

|

tom 15

|

nr 3

14

Inhibition of 2-oxoglutarate metabolism by lithium in the isolated rat kidney cortex tubules

51%

Stepinski J., Rybczynska A., Angielski S.

Acta Biochimica Polonica

|

1984

|

tom 31

|

nr 2

15

Co-operation between particulate and soluble guanylyl cyclase systems in the rat renal glomeruli

51%

Stepinski J., Wendt U., Lewko B., Angielski S.

Journal of Physiology and Pharmacology

|

2000

|

tom 51

|

nr 3

16

Mechanical stress decreases activity of particulate guanylyl cyclase in cultured podocytes

45%

Lewko B., Stepinski J., Endlich N., Kriz W., Endlich K.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr Supplement 1

17

Adenosine metabolism in real cortex and medulla

45%

Bizon D., Wojnowski L., Pawelczyk T., Stepinski J., Angielski S.

Acta Physiologica Polonica

|

1988

|

tom 39

|

nr 1

18

Plant nucleoside 5'-phosphoramidate hydrolase; simple purification from yellow lupin (Lupinus luteus) seeds and properties of homogeneous enzyme

45%

Guranowski A., Wojdyla A., Rydzik A., Stepinski J., Jemielity J.

Acta Biochimica Polonica

|

2011

|

tom 58

|

nr 1

Adenosine 5'-phosphoramidate (NH2-pA) is an uncommon natural nucleotide of poorly understood biochemistry and function. We studied a plant enzyme potentially involved in the catabolism of NH2-pA. A fast and simple method comprising extraction of yellow lupin (Lupinus luteus) seed-meal with a low ionic strength buffer, ammonium sulfate and acetone fractionations, removal of contaminating proteins by heat denaturation, and affinity chromatography on AMP-agarose, yielded homogenous nucleoside 5'-phosphoramidase. Mass spectrometric analysis showed that the lupin hydrolase exhibits closest similarity to Arabidopsis thaliana Hint1 protein. The substrate specificity of the lupin enzyme, in particular its ability to split the P-S bond in adenosine 5'-phosphorothioate, is typical of known Hint1 proteins. Adenosine 5'-phosphofluoride and various derivatives of guanosine 5'-phosphoramidate were also substrates. Neither common divalent metal cations nor 10 mM EDTA or EGTA affected the hydrolysis of NH2-pA. The enzyme functions as a homodimer (2 × 15 800 Da). At the optimum pH of 7.0, the Km for NH2-pA was 0.5 µM and kcat 0.8 s-1 (per monomer active site). The properties of the lupin nucleoside 5'-phosphoramidase are compared with those of its counterparts from other organisms.

19

Mechanical stress and glucose modulate glucose transport in podocytes

45%

Lewko B., Bryl E., Witkowski J., Stepinski J., Angielski S.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr Supplement 1

20

Regulation of cGMP synthesis in cultured podocytes by vasoactive hormones

45%

Lewko B., Golos M., Latawiec E., Angielski S., Stepinski J.

Journal of Physiology and Pharmacology

|

2006

|

tom 57

|

nr 4

The podocytes are highly differentiated cells playing a key role in glomerular filtration. Vasoactive factors including angiotensin II (Ang II) and cyclic guanosine 5' monophosphate (cGMP) are synthesized by these cells upon stimulation as well as in the basal state. In this study we have tested whether angiotensin II affects the total synthesis of cGMP in primary culture of rat podocytes. The cells were stimulated with atrial natriuretic peptide (ANP) and/or a nitric oxide (NO) donor, S-nitroso-N-acetyl penicillamine (SNAP), in the absence or presence of Ang II. The cGMP synthesis was determined by radioimmunoassay (RIA). ANP or SNAP alone increased the cGMP synthesis in podocytes although the effects were not additive unless Ang II was present in the medium. Ang II suppressed the ANP-dependent cGMP synthesis whereas SNAP-dependent cGMP production remained unaffected. These effects were prevented by a non-specific antagonist of Ang II receptors (AT), saralasin. Adversely, PD123319, a specific inhibitor of AT2 receptors, augmented inhibition of ANP-dependent and enhanced the NO-dependent cGMP production. Probenecid, an inhibitor of cGMP extrusion from the cells, suppressed the cGMP generation by both ANP and SNAP. We conclude that cGMP synthesis in cultured podocytes is modulated by angiotensin II and that two adversely acting receptors, AT1 and AT2 are involved in this effect. Additionally, production of cGMP might be intrinsically inhibited by cGMP accumulating inside the cells.

Ograniczanie wyników

Wpływ litu i zaburzeń równowagi kwasowo-zasadowej na przemiany i transport metabolitów cyklu Krebsa w nerkach

Wazniejsze obiekty historyczne w Nadwarcianskim Parku Krajobrazowym

W obronie Warty - lokalna inicjatywa samorzadowa

Regulation of aconitate hydratase activity from rat kidney cortex by bicarbonate

Radioimmunologiczna metoda oznaczania adenozyny

Effect of lithium on renal gluconeogenesis

Renal function in acetate treated rats

Synthesis of nucleosides tri- and tetraphosphates resistant to hydrolysis

Inhibition of alfa-oxoglutarate and pyruvate oxidation by alfa-oxoderivatives of leucine and valine in rat tissues

Badania oddziaływania pomiędzy m7GMP a modelowym peptydem Trp-Len-Glu metodą wygaszania fluorescencji

Effect of dipyridamole and furosemide on rental function during adenine dinucleotide infusion in rats

Type 2 (AT2) angiotensin II receptor modulates guanylyl cyclase activities in rat podocytes

Zwiększenie przez sulfonylomocznik, SPC-703, powinowactwa insuliny do jej receptorów w kanalikach nerkowych szczura

Inhibition of 2-oxoglutarate metabolism by lithium in the isolated rat kidney cortex tubules

Co-operation between particulate and soluble guanylyl cyclase systems in the rat renal glomeruli

Mechanical stress decreases activity of particulate guanylyl cyclase in cultured podocytes

Adenosine metabolism in real cortex and medulla

Plant nucleoside 5'-phosphoramidate hydrolase; simple purification from yellow lupin (Lupinus luteus) seeds and properties of homogeneous enzyme

Mechanical stress and glucose modulate glucose transport in podocytes

Regulation of cGMP synthesis in cultured podocytes by vasoactive hormones