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Plantlets of Inula verbascifolia ssp. aschersoniana regenerated in the in vitro cultures of seedling shoot tips and shoot-derived callus tissue. The in vitro cultures were initiated from aseptically germinated seedlings. Shoots were multiplied on MS media containing 2.22 (iM of 6-benzyladenine (BA) and 0.26 |iM of a-naphthaleneacetic acid (NAA) or 18.6 (tM of kinetin and 19.6 nM of indole 3-butyric acid (IBA). As a result of indirect organo­genesis, adventitious shoots were obtained from callus on a medium with BA (0.88 fiM). Conditions of cell suspension culture from a friable, light callus tissue have been deter­mined. Shoot rooting appeared to be the best after the addition of IBA (0.98 ^iM) to the same basal media. Rooted, phenotypicaily uniform plantlets were transferred to potting soil and acclimatized to outdoor conditions. The presence and content of parthenolide in the shoot culture, callus tissue and cell suspension culture, were determined by TLC and HPLC methods. In contrast to shoots containing fairly 0.43% parthenolide, only trace amounts of this compound were found in the callus tissue and cell suspension culture.
The occurrence of syringin in eight out of ten investigated plant species of the subtribe Centaureinae (Asteraceae) was reported. The compound was isolated from aerial parts of the plants by silica gel column chromatography of methanol extracts. Contents of syringin varied from 0.001 to 0.1% of the dried plant material. Centaurea bella Trautv. appeared to be the best source of this compound (0.1% dry wt.). Syringin has been reported for the first time from these plant species.
The study aimed at determining whether teeth defects in chinchillas (Chinchilla laniger), consisting in excessive growth of I, P, and M1-M3 teeth, bring about differences in the value of cranial breadth-length traits in those rodents. The examinations were made on 109 skulls of somatically mature chinchilla females aged 1 to 3 years. The skull's weight and that of mandible was determined, as well as 48 craniometrical traits of the braincase and the splanchno-cranium was measured. For each craniometrical trait, its mean value, standard deviation, and minimum and maximum values were determined, as well as cranial indices and simple correlations between the traits were calculated. Statistical analysis did not show normal distribution of traits. It was showed that the mean skull weight as well as that of the mandible in chinchillas without teeth defects was significantly lower than in animals with such defects. On the other hand, teeth defects brought about differences in the basic values of metrical traits for the lateral part of skull, basal skull, and mandible. No differences were found in the values of the nuchal part traits of the examined chinchilla skulls.
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