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1

Cykl infekcyjny wirusa pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 04
232-236
Foot-and-mouth disease virus (FMDV) is a single-stranded, positive-sense RNA virus belonging to the genus Aphthovirus in the family Picornaviridae. FMDV enters cells via the mechanism of receptor-mediated endocytosis in which the low pH of the endosomal compartment triggers uncoating of the viral genome. FMDV enters cells by attaching itself to cellular receptor molecules of the integrin family. For FMDV the receptor has been identified as the Arg-Gly-Asp (RGD) binding integrin. The integrin-binding RGD is located in the G-H loop of VP1 and it is highly conserved among all seven serotypes. The FMDV genome organization is similar to that of other picornaviruses. The genome is composed of three parts, the 5’ non-translated region (5’NTR), the coding region and the 3’ non-translated region (3’NTR) containing a heteropolymeric segment and poly(A) tail, which is required for viral replication. The 5’NTR plays important roles in cap-independent translation initiation of the viral polyprotein and in viral genome replication. After translation, the polyprotein is cleaved into four primary cleavage products: the amino terminal L protease; P1-2A, the precursor of the capsid proteins; 2BC and P3 which are cleaved into nonstructural proteins.
2

Wirus pryszczycy i jego budowa molekularna

100%
Paprocka G.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 07
753-756
Foot-and-mouth disease virus (FMDV) is an important animal pathogen that belongs to the Aphthovirus genus of the Picornaviridae family and infects cattle and other cloven-hoofed animals. Seven serotypes (A, O, C, Asia1, SAT1, SAT2 and SAT3) have been identified serologically, and multiple subtypes occur within each serotype. FMDV enters cells by receptor-mediated endocytosis. By electron microscopy the FMD virion appears to be a round particle with a smooth surface and a diameter of about 25 nm. The FMD viral particle contains a positive-strand RNA genome of about 8500 nucleotides, enclosed within a protein capsid. The virus capsid is made up from 60 copies each of four virus-encoded proteins VP1 to VP4. The FMDV genome is composed of the 5’ non-translated region (5’NTR), the coding region, and the 3’ non-translated region (3’NTR). The genome encodes a single polyprotein, from which the different viral polypeptides are derived by viral proteases. FMDV populations are genetically and anti-genetically heterogeneous. FMDV have very high mutation rates.
3

Izolacja i identyfikacja wirusa pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 12
1404-1406
Foot-and-mouth disease (FMD) is the most contagious disease of mammals and has great potential for causing severe economic losses in susceptible cloven-hoofed animals. FMD is caused by a virus of the genus Aphthovirus, family Picornaviridae. Serological tests in laboratories have identified seven different serotypes as O, A, C, SAT 1, SAT 2, SAT 3 and Asia 1. FMD is diagnosed by the virus isolation or demonstration of FMD viral antigen or nucleic acid in samples of biological specimens. The purpose of the study was to apply the isolation test in cell culture and a RT-PCR assay for the detection of foot-and-mouth disease virus in biological materials. Out of the total of 14 examined samples, 6 (42.8%) were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of the isolation assay. Primary bovine thyroid cells were found the most sensitive cell culture system for the detection of foot-and-mouth disease virus, followed by secondary lamb kidney and certain IB-RS-2 cells.
4

Przydatnosc RT-PCR w diagnostyce pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 09
621-623
Foot-and-mouth disease virus (FMDV) from the family Picornaviridae, genus Aphthovirus, exists in the form of seven different serotypes: O, A, C, Asia 1 and SAT 1-3. Infection with one serotype does not confer immunity against another. Foot-and-mouth disease is one of the greatest threats to animal health in European countries. The rapid and accurate detection of FMDV is of the utmost importance. The RT-PCR assay was used to detect the presence of FMDV in samples. Positive results of the RT-PCR assay were found in all samples and in the positive control, the negative control reacted negatively. No cytopathic effects in primary bovine thyroid cells were observed in 2 samples that had been thawed several times. The reference strains of FMDV was used to determine the sensitivity of the test. The sensitivity of RT-PCR for detection of FMDV (serotype O, A) was 1 TCID₅₀ and 10 TCID₅₀ (serotype C, Asia 1) by gel electrophoresis.
5

Skuteczność wykrywania RNA wirusa pryszczycy metodą PCR w zależności od starterów używanych do syntezy cDNA

100%
Paprocka G.
Biotechnologia
|
1998
|
nr 3
6

Zastosowanie hodowli pierwotnej komorek nerki jagniecia w diagnostyce pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 03
332-334
Sensitive cell cultures, such as primary bovine thyroid cells and primary pig, calf or lamb kidney cells can be used for isolating foot-and-mouth disease virus (FMDV). Established cell lines IB-RS-2 and BHK-21 may also be applied for this purpose. The aim of this study was to assess the efficacy of primary lamb kidney cell culture for detecting FMDV in biological materials. The results of the study demonstrate that this cell culture may be a useful tool in diagnostic studies of FMD.
7

Szczepionki przeciwko pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 11
Foot-and-mouth disease (FMD) is considered one of the most contagious and economically devastating diseases affecting cloven-hoofed livestock worldwide. The etiologic agent, FMD virus (FMDV), has a positive-sense, single-stranded RNA genome, and belongs to the genus Aphthovirus in the family Picornaviridae. FMDV is antigenically variable and consists of seven serotypes (A, O, C, Asia 1, SAT 1, SAT 2, SAT 3) and more than 60 subtypes. Antigenic diversity of FMDV is a major concern for FMD control. An important part of controlling and prevention of foot and mouth disease are still conventional inactivated vaccines. Vaccines against FMD are of major importance in endemic regions for controlling the disease, they are also important as emergency vaccines to limit the spread of outbreaks in FMD-free countries. Inactivated FMD preparations have been used successfully as part of eradication programs. However there are many problems and limitations associated with their use. In order to solve them a new generation vaccines is being developed. The article presents various types of FMD vaccines such as inactivated vaccines, subunit vaccines, live vector vaccines, DNA vaccines and live attenuated vaccines.
8

Ocena namnażania wirusa pryszczycy w hodowli komórek oraz środków inaktywujących e celu uzyskania skutecznej szczepionki przeciwpryszczycowej

100%
Paprocka G.
Medycyna Weterynaryjna
|
1984
|
tom 40
|
nr 11
9

Patogeneza pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 10
1180-1183
Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease of cloven-hoofed animals of the Artiodactyla order. The disease is characterized by fever, lameness and vesicular lesions on the tongue, feet, snout and teats. It is generally accepted that primary infection of ruminants usually occurs by the respiratory route, whereas pigs are usually infected by the oral route. Pigs are much less susceptible to aerosol infection than cattle, yet they excrete far more aerosolized virus than cattle or sheep. In addition, cattle, sheep, and goats can become carriers. The virus elicits a rapid humoral response in either infected or vaccinated animals Virus-specific antibodies protect animals in a serotype-specific manner against reinfection, or against infection in the case of vaccination. Protection is correlated with a high levels of neutralizing antibodies. The role of cellular immunity in the protection of animals from FMD is still a matter of some controversy.
10

Badania molekularne serotypow wirusa pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 07
778-782
The foot-and-mouth disease virus (FMDV) belongs to the family of Picornaviridae, genus Aphthovirus. The virus exists in the form of seven different serotypes: O, A, C, Asia 1, SAT 1, SAT 2, SAT 3 and multiple subtypes reflecting significant genetic variability. The FMDV genome organization is similar to that of other picornaviruses. The determination of FMDV nucleotide sequences and phylogenetic analysis is the definitive technique for characterizing individual isolates of the virus. The paper presents information regarding genetic studies of FMDVs originating from different parts of the world.
11

Postep w laboratoryjnej diagnostyce pryszczycy

100%
Paprocka G.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 08
706-709
12

Wykrywanie wirusa choroby pecherzykowej swin w materiale biologicznym

100%
Paprocka G.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 02
118-120
Swine vesicular disease virus (SVDV) is a member of the genus Enterovirus in the family Picornaviridae. This virus appears to have evolved from human coxsackievirus B5. Pigs infected with this virus show almost identical clinical signs to foot-and-mouth disease in pigs. Vesicular diseases must be differentiated with laboratory tests. The purpose of the study was to apply the isolation test in cell culture and RT-PCR assay for the detection of swine vesicular disease virus in the epithelial and fecal samples. Out of a total of 11 examined samples, 10 were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of isolation assay. Primary piglet kidney cells and certain IB-RS-2 cells were a sensitive cell culture system for the detection of swine vesicular disease virus, whereas secondary lamb kidney cells not.
13

Genetic analysis of serotype O of foot-and-mouth disease virus

100%
Paprocka G.
Bulletin of the Veterinary Institute in Puławy
|
2004
|
tom 48
|
nr 3
14

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

63%
Paprocka G., Kesy A.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 4
A swine vesicular disease virus (SVDV) replication assay in IB-RS-2, SK-6, and PK-15 cell cultures was performed using the xCELLigence system. The cell status was monitored by impedance measurement, expressed as cell index (CI). Proliferation of particular cells was examined at the beginning of the study. The cells exhibited the ability to form a monolayer, and the CI values increased with the cell culture growth. After about 23 h and while still in the growth phase, the cells were infected with decimal virus dilutions (10⁻¹-10⁻⁶) containing from 100 000 to 1 median tissue culture infectious doses (TCID₅₀). SVDV replication in cell cultures induced a change in cell index; together with the occurrence of cytopathic effect (CPE), the CI values declined. A significant correlation between the concentration of the virus used and CPE occurrence was found. The results also enabled determination of cell sensitivity to SVDV infection. The highest sensitivity was exhibited by IB-RS-2, followed by SK-6. To conclude, the xCELLigence System was used effectively and evaluated as being an efficient tool for CPE detection and SVDV replication analysis in cell cultures. Compared to the standard method, it enabled a more precise assessment of viral replication based on the quantitative CI measurement, providing additional current information.
15

Pryszczyca - wciąż aktualne zagrożenie

63%
Paprocka G., Kesy A.
Magazyn Weterynaryjny
|
2011
|
tom 20
|
nr 02
16

Szczepionki przeciwko pryszczycy i perspektywy ich rozwoju

63%
Paprocka G., Kesy A.
Biotechnologia
|
1993
|
nr 2
97-104
17

Postęp w dziedzinie badań nad pryszczycą

63%
Paprocka G., Kesy A.
Postępy Mikrobiologii
|
1986
|
tom 25
|
nr 1-2
18

Zastosowanie sondy molekularnej do wykrywania wirusa pryszczycy

63%
Paprocka G., Kesy A.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 03
227-229
19

Wybrane choroby uwzględniane w diagnostyce różnicowej pryszczycy

63%
Paprocka G., Fitzner A.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 08
s.538-543,rys.,tab.,bibliogr.
A number of diseases have similar or identical clinical symptoms as does foot-and-mouth disease, including swine vesicular disease (SVD), vesicular stomatitis (VS), rinderpest (RP) and peste des petits ruminants (PPR). SVD is an acute, highly contagious viral disease of pigs caused by a virus belonging to the genus Enterovirus in the family Picornaviridae. VS is a vesicular disease of horses, cattle and pigs caused by vesiculoviruses of the family Rhabdoviridae. RP and PPR are acute viral diseases caused by the Morbillivirus genus within the family Paramyxoviridae. Classic descriptions of RP refer to it as a highly fatal disease of domestic cattle, buffaloes and yaks. PPR affects sheep and goats and occasionally small wild ruminants. Laboratory investigations are a key to their precise diagnosis. The most important data regarding these diseases are presented in this article.
20

Pryszczyca - wystepowanie i zwalczanie choroby

63%
Paprocka G., Kesy A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 03
251-253
The review presents a short description of foot-and-mouth disease (FMD) - a highly contagious disease and important from the economic point of view. It describes the clinical symptoms of FMD and the present global FMD situation as well as control measures. In addition it describes the latest fast diagnostic assays.
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