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Morphogenetic response to plant growth regulators in transformed and untransformed Hypericum perforatum L. clones

100%

Koperdakova J., Katkovcinova Z., Kosuth J., Giovannini A., Cellarova E.

Acta Biologica Cracoviensia. Series Botanica

2009

tom 51

nr 1

61-70

This study examined the effects of different exogenous auxins and cytokinins at 0.1-5.0 mg·l-1 concentration on shoot cuttings of two H. perforatum clones transformed with a wild agropine strain of A. rhizogenes and one untransformed clone. Their sensitivity to the auxins varied and showed concentration-dependent behavior, and the response to auxins differed between the transgenic clones. The number of cuttings of transgenic clones capable of root formation, and the onset of rooting on most of the media with auxins lagged behind the control. The number of differentiated shoots of the transgenic clones on hormone-free medium was two to three times higher than that of the untransformed control. Regenerated shoots of the transgenic clones on basal medium branched much less than the nontransgenic clone. The transgenic and control clones differed in their ability to form shoots on media supplemented with cytokinins. Increased cytokinins led to differentiation of shorter shoots with fewer leaf pairs. Because gene expression studies have shown integration of rolABC genes, their possible impact on the type of morphogenetic response is discussed.

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

100%

Koperdakova J., Komarovska H., Kosuth J., Giovannini A., Cellarova E.

Acta Physiologiae Plantarum

2009

tom 31

nr 2

Hairy root-regenerated clones of Hypericum perforatum L. grown in vitro similarly to those successfully adapted to ex vitro conditions showed phenotype features typical for plants transformed with Agrobacterium rhizogenes T-DNA. These included reduced apical dominance, increased branching, dwarfing and reduced fertility. Transgenic clones differed in ability to develop root system as a necessary condition for transfer to the soil. One of the profiling characters, capability of hypericin biosynthesis was altered as well. Dark glands as the sites of hypericin accumulation and/or synthesis exhibited significantly higher densities on both, leaves and petals of transgenic clones comparing to controls. In the genome of transgenic clones, rolABC genes were detected. Both clones harboured similar copy number of individual rol genes. However, copy numbers descended from rolA to rolC gene in both clones.

Genotoxic effects due to in vitro culture and H2O2 treatments in Petunia x hybrida cells monitored through DNA diffusion assay, FPG-SCGE and gene expression profile analyses

72%

Ventura L., Macovei A., Dona M., Paparella S., Buttafava A., Giovannini A., Carbonera D., Balestrazzi A.

Acta Physiologiae Plantarum

2014

tom 36

nr 02

In the present work, Petunia × hybrida leaf discs maintained on regeneration medium for 8 days were used to assess the effects of genotoxic stress induced by in vitro culture. The investigation was carried out by comparing the response of intact leaves excised from Petunia × hybrida plantlets grown in vitro and the regenerating leaf discs. In situ detection by histochemical staining and alkaline-Single Cell Gel Electrophoresis (SCGE) analysis demonstrated that both reactive oxygen species accumulation and DNA damage were enhanced in explants cultured in vitro. Significant up-regulation of the PhOGG1 (8-oxoguanine DNA glycosylase/lyase), PhAPX (ascorbate peroxidase) and PhMT2 (metallothionein) genes involved in DNA repair and antioxidant defence was observed in the explants cultured in vitro, compared to intact leaves. The Petunia × hybrida leaf discs were exposed to increasing (0, 100, 150, 200 and 400 mM) doses of the model genotoxic agent hydrogen peroxide (H₂O₂) and then analysed. The DNA diffusion assay highlighted the dose- and time-dependent fluctuations of programmed cell death/necrosis events in response to H₂O₂. Leaf discs treated with increasing H₂O₂ concentration and untreated controls were analysed by FPG-SCGE to assess the level of oxidative DNA damage at different time points following treatments. The PhOGG1, PhAPX and PhMT2 genes were significantly up-regulated in response to H₂O₂, reaching the highest transcript levels with the 150 mM dose. Based on the reported data, these genes might be used as molecular indicators of the genotoxic stress response in Petunia × hybrida cells.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

1 Acta Biologica Cracoviensia. Series Botanica

3 Giovannini A.

2 Cellarova E.

2 Koperdakova J.

2 Kosuth J.

1 Balestrazzi A.

1 Buttafava A.

1 Carbonera D.

1 Dona M.

1 Katkovcinova Z.

1 Komarovska H.

1 Macovei A.

1 Paparella S.

1 Ventura L.

1 2014

2 2009

Wyniki wyszukiwania

Morphogenetic response to plant growth regulators in transformed and untransformed Hypericum perforatum L. clones

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

Genotoxic effects due to in vitro culture and H2O2 treatments in Petunia x hybrida cells monitored through DNA diffusion assay, FPG-SCGE and gene expression profile analyses