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1

Effects of cold air on serum catecholamine levels in patients with cardiovascular or cerebrovascular disease

100%
Ruan Y., Zhang L., Niu J., Wang S.
Polish Journal of Environmental Studies
|
2013
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tom 22
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nr 5
This study analyzes the effects of cold air on catecholamine (CA) levels. The CA levels of patients with cardio-cerebrovascular diseases, healthy people, Wistar rats, and spontaneously hypertensive (SHR) rats were determined before, during, and after cold air activity. The levels of dopamine (DA), norepinephrine (NE), and adrenaline (AD in humans and experimental animals changed in all three periods of cold air activity. The change in DA levels was statistically significant (P<0.05). The DA, NE, and AD levels in the controls and the Wistar rats increased during cold air activity and decreased after cold air activity. The variation in CA levels was not exactly the same between the SHR rats and the cardio-cerebrovascular disease patients. The special meteorological conditions caused by cold air affects CA secretion, which induces the occurrence, development, and outcome of cardio-cerebrovascular diseases. Moreover, the results of the animal experiments could not be directly extrapolated to humans.
2

iTRAQ-based proteomic analysis provides insights into the biological mechanism of ammonium metabolism in tea plant (Camellia sinensis L.)

100%
Liu M.-Y., Tang D., Zhang Q., Ruan Y.
Acta Physiologiae Plantarum
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2020
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tom 42
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nr 04
3

Comparison of the effects of NaCl and KCl at the roots on seedling growth, cell death and the size, frequency and secretion rate of salt glands in leaves of Limonium sinense

100%
Ding F., Song J., Ruan Y., Wang B.
Acta Physiologiae Plantarum
|
2009
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tom 31
|
nr 2
Twenty days’ exposure to 50 or 100 mM NaCl in the rooting medium substantially increased fresh and dry weights of seedling shoots of the recretohalophyte Limonium sinense while 200 or 300 mM were increasingly inhibitory. KCl treatment was only slightly stimulating (50 mM) or strongly inhibitory (100–300 mM). Lesser effects on leaf area were also seen. Diameter of foliar salt glands was significantly larger than that of controls in 100 and 200 mM NaCl with the effect being reversed at higher concentrations. Gland enlargement was also observed in the presence of 100 mM KCl, while larger concentrations reduced gland size. Generally, gland diameter was larger in the presence of NaCl than in KCl. NaCl and KCl also increased gland number per leaf and secretion rate per gland. At 100 and 200 mM NaCl or KCl, Na⁺ secretion per leaf from NaCl-treated plants exceeded K⁺ secretion rate from KCl-treated plants while at 200 mM, Na⁺ secretion per gland was significantly higher for Na⁺ than for K⁺. Evidence of cell death in leaves of salt-treated plants using Evans blue staining indicates that release of cell contents through loss of membrane integrity contributed to the secretion values. We conclude that the greater tolerance of L. sinenseto to NaCl compared to KCl is linked to the more effective secretion of Na⁺ than of K⁺ and, in turn, to a greater stimulation of salt gland formation and activity and larger gland diameter.
4

Highly efficient Agrobacterium-based transformation sysyem for callus cells of the C3 halophyte Suaeda salsa

100%
Zhao S.-Z., Ruan Y., Sun H.-Z., Wang B.-S.
Acta Physiologiae Plantarum
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2008
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tom 30
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nr 5
Genetic manipulation technologies have been limited in the halophyte Suaeda salsa L. due to the lack of an efficient transformation system. Here, we examined factors affecting transformation and developed an efficient transformation system at the cell level using S. salsa hypocotyl as starting material. S. salsa hypocotyl explants from 10-day-old seedlings were precultured for 2 days on a hygromycin (hyg)-free callus induction medium (CIM) and then inoculated with Agrobacterium tumefaciens suspension at a concentration of 0.5 at OD600 for 5–10 min. After cocultivation with A. tumefaciens for 4 days in the dark, followed by selection on carbenicillin (carb) for 3 days, explants were placed on CIM containing 10 mg l⁻¹ hyg and 500 mg l⁻¹-carb with three to four consecutive subcultures for up to 45 days. β-Glucuronidase assays showed an average transformation frequency of 62.89%. Gene integration was confirmed by polymerase chain reaction analysis and Northern blot analysis. To our knowledge, this is the first study to show Agrobacterium-mediated transformation in the C3 halophyte S. salsa.
5

A new species of Dima (Coleoptera: Elateridae: Dimini), with a checklist and identification key to the Chinese species

100%
Qiu L., Sormova E., Ruan Y., Kundrata R.
Annales Zoologici
|
2018
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tom 68
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nr 3
6

Epigenetic control of Pollen Ole e 1 allergen and extensin family gene expression in Arabidopsis thaliana

76%
Hu B., Liu B., Liu L., Liu C., Xu L., Ruan Y.
Acta Physiologiae Plantarum
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2014
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tom 36
|
nr 08
Pollen Ole e 1 domain-containing proteins were initially identified as major allergens. However, recent studies have suggested that these proteins also function as developmental regulators in many other plant tissues. To understand the possible roles of these proteins in plant development, we characterized 28 Pollen Ole e 1 allergen and extensin (AtPOE1) family proteins from Arabidopsis thaliana, and analyzed the transcript levels of the genes encoding these proteins in various tissues. Our analysis revealed that most of the AtPOE1 family genes showed spatial transcription patterns, suggesting that they may be involved in various events at diverse developmental stages. Using one member of the family, AtPOE1;26, as a model, we showed that it is mainly expressed in the root. In addition, the AtPOE1;26 gene locus has a significant histone H3 lysine 27 trimethylation (H3K27me3) modification, which is the typical epigenetic regulation marker of the Polycomb group (PcG) pathway. AtPOE1;26 transcription was upregulated in the PcG mutant curly leaf (clf), and the H3K27me3 level at the AtPOE1;26 locus was reduced in clf, suggesting that its expression is controlled by PcG. Besides AtPOE1;26, the gene loci of at least 13 AtPOE1 family members were modified by H3K27me3. Overall, these data indicate that AtPOE1 family members have spatial expression patterns in multiple plant tissues, and that their expression could be epigenetically controlled.
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