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1

Produkcja metabolitow wtornych w transformowanych kulturach roslin leczniczych

100%
Wysokinska H.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
1997
|
tom 50
87-92
2

Wytwarzanie metabolitów wtórnych w kulturach korzeni transformowanych

100%
Wysokinska H.
Biotechnologia
|
2000
|
nr 4
3

Modyfikacje genetyczne dróg wtórnego metabolizmu w roślinnych kulturach in vitro

100%
Wysokinska H.
Biotechnologia
|
2000
|
nr 4
4

Selekcja linii komorkowych Penstemon serrulatus z podwyzszona zawartoscia glukozydow irydoidowych

100%
Wysokinska H.
Biotechnologia
|
1992
|
nr 4
41-49
5

Wytwarzanie metabolitów wtórnych w pędach Salvia nemorosa L. otrzymanych metodą in vitro

63%
Kuzma L., Wysokinska H.
Biotechnologia
|
2003
|
nr 4
6

Tanshinone production in roots of micropropagated Salvia przewalskii Maxim.

63%
Skala E., Wysokinska H.
Herba Polonica. Suplement
|
2005
|
tom 51
|
nr 1
7

Biotransformacje w kulturach komorek roslinnych. Cz.II. Warunki procesow biotransformacji

63%
Chmiel A., Wysokinska H.
Biotechnologia
|
1995
|
nr 1
131-147
8

The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L.

63%
Grzegorczyk I., Wysokinska H.
Herba Polonica
|
2009
|
tom 55
|
nr 3
238-243
The shoots of Salvia officinalis growing in MS liquid medium supplemented with IAA 0.1 mg l-1) and BAP (0.45 mg l-1) were treated with methyl jasmonate (MeJA) to increase production of compounds with antioxidant activity (carnosic acid, carnosol and rosmarinic acid). The increase in metabolite production depended on MeJA concentration, the period of exposure to elicitor and type of compound. The MeJA action was observed 24 h after elicitation. It was found that the maximum level of diterpenoids, calculated as the sum of CA and Car (about 8 mg g-1 dry wt) was achieved at 3 days after elicitation with 20 µM methyl jasmonate. The highest amount of rosmarinic acid (about 41 mg g-1 dry wt) was achieved with 50 or 100 μM methyl jasmonate on the 5th day after elicitation. It was almost 2-fold higher compared to the control (cultures treated with only ethanol).
9

Mikrorozmnażanie Arnica montana L. i wytwarzanie laktonów seskwiterpenowych w uzyskanych roślinach

63%
Weremczuk-Jezyna I., Wysokinska H.
Biotechnologia
|
2000
|
nr 4
10

Micropropagation of Harpagophytum procumbens [Burch.] DC. ex Meisn.: the effect of cytokinins on shoot multiplication

63%
Grabkowska R., Wysokinska H.
Herba Polonica
|
2009
|
tom 55
|
nr 3
244-250
The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 µM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 µM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 µM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3 ) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months.
11

Organ and plantlet regeneration of Menyanthes trifoliata through tissue culture

63%
Adamczyk-Rogozinska U., Wysokinska H.
Acta Societatis Botanicorum Poloniae
|
1998
|
tom 67
|
nr 2
12

Micropropagation of Scutellaria altissima L

63%
Grzegorczyk I., Wysokinska H.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 1
13

Oxycoccus quadripetalus Gilib. w kulturze in vitro

63%
Floryanowicz-Czekalska K., Wysokinska H.
Biotechnologia
|
2004
|
nr 2
225-230
14

Establishment of hairy roots of Rehmannia glutinosa Libosch. By agrobacterium rhizogenes and iridoid and content of phenyle- thanoid glycosides in obtained cultures

63%
Piatczak E., Wysokinska H.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr Suppl.1
15

Kultury korzeni transformowanych Arnica montana L. - izolacja metabolitow wtornych

63%
Weremczak-Jezyna I., Wysokinska H.
Biotechnologia
|
2004
|
nr 2
54-60
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Encapsulation of Centaurium Erythraea Rafn – an Efficient Method for Regeneration of Transgenic Plants

63%
Piatczak E., Wysokinska H.
Acta Biologica Cracoviensia. Series Botanica
|
2013
|
tom 55
|
nr 2
17

Produkcja roslinnych metabolitow wtornych w kulturach organow transformowanych

63%
Wysokinska H., Chmiel A.
Biotechnologia
|
2006
|
nr 4
124-135
18

Mikrorozmnazanie Salvia officinalis L. z wierzcholkow pedow

63%
Grzegorczyk I., Wysokinska H.
Biotechnologia
|
2004
|
nr 2
212-218
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

A protocol for synthetic seeds from Salvia officinalis L. shoot tips

63%
Grzegorczyk I., Wysokinska H.
Acta Biologica Cracoviensia. Series Botanica
|
2011
|
tom 53
|
nr 1
Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3- acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA3). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor

63%
Grzegorczyk I., Wysokinska H.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr 1
7-10
The study focused on the production of compounds with antioxidant activity in hairy root and shoot cultures of Salvia officinalis grown in laboratory-scale sprinkle nutrient bioreactors. HPLC analysis showed that production of rosmarinic acid in transformed roots (34.65±1.07 mg l-1) was higher that in shoot culture (26.24±0.48 mg l-1). In the latter diterpenoids: carnosic acid (1.74±0.02 mg l-1) and carnosol (1.34±0.01 mg l-1) were also found. Biomass accumulation after a growth period in the bioreactor was also studied. An 18-fold increase in hairy root biomass was recorded after 40 days of culture. In sage shoot culture, biomass increased 43 times after 21 days of bioreactor run. The current operating conditions of the bioreactor were not suitable for the propagation of Salvia officinalis mainly due to the hyperhydricity problem of leaves and stems.
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