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Badano wpływ zwiększonego promieniowania UV-B (16 kJ/m2 w ciągu doby) na poziom barwników chloroplastowych, intensywność fotosyntezy i wzrost siewek ogórka odmiany mieszańcowej ‘Polan’ F1 w dwu stadiach rozwojowych. W liścieniach pod wpływem zwiększonej radiacji poziom barwników chloroplastowych i intensywność fotosyntezy wzrastały w porównaniu z kontrolą. W liściach siewek 3-tygodniowych w warunkach stresowych stwierdzono sukcesywny spadek zawartości barwników, intensywności fotosyntezy oraz ograniczenie wzrostu.
In the last few years on old yew trees growing in the parks and gardens extensive disease symptoms were observed on leaves, indicating affection with pathogenic microorganisms. The causal factor was the presence of a fungal pathogen, Pestalotiopsis funerea. Physiological responses involving the oxidative stress, i. e. superoxide anion and hydrogen peroxide levels, and the superoxide dismutase were analyzed as an element of a plant defense mechanism. Diseased leaves from plants growing under sunny and shaded conditions, from May to July, were investigated. The increased generation of superoxide anion-radicals and hydrogen peroxide were observed in the leaves with disease symptoms simultaneously with the activation of superoxide dismutase, which may indicate the induction of host defense response to the P. funerea.
The combined effects of enhanced UV-B radiation and soil drought on antioxidant enzyme activity were investigated in cucumber leaves. One-month-old cucumber plants (Cucumis sativus cv. Dar) were exposed to UV-B irradiation and water deficit alone or combined. Physiological measurements were made in seedlings kept under stress conditions for nine days and then two more days with stresses withdrawn. Generally a decrease in relative water content and an increase in dry weight content were recorded. The more significant changes were observed under drought than under UV-B radiation and or combined UV-B and drought. Both stresses stimulated antioxidant enzyme activity. Superoxide dismutase activity increased earlier (day 2) than guaiacol peroxidase and glutathione reductase activity (days 5 and 7). Elevation of enzyme activities was higher under drought than under UV-B. Combined UV-B and drought functioned synergistically: one of the stresses reduced the effects caused by simultaneous application of the other.
Lupine diseases caused by pathogenic fungi constitute a serious problem in agriculture. They lead to partial yield loss and deterioration of crop quality through the changes in biochemical composition of seeds or their contamination with mycotoxins. Some of common lupine diseases are fusarioses caused by Fusarium oxyspomm f. sp. lupini. Morphometric and metabolic changes were investigated in yellow lupine seedlings infected with F. oxyspomm f. sp. lupini. It was found that infection caused temporary inhibition of seedling growth, overcome at later development, and activation of ß-glycosidase and peroxidases. The changes in enzymes activity indicate the induction of defense mechanism against F. oxyspomm f. sp. lupini and inhibition of pathogen spread.
Field experiment was carried out on the Didactic and Experimental Farm in Swadzim (52°26’ N; 16°45’ E) near Poznań, in the years 2004-2007. The three-factorial experiment was established in “split-plot” design with 4 replications. The reaction of two maize hybrid types on the application of 6 nitrogen doses and magnesium fertilization was studied. The influence of the studied factors on the content of chloroplast pigments in maize leaf blades in the phase of ear blooming (BBCH 67) was estimated. A greater content of chlorophyll a and a + b expressed in SPAD units was found in maize leaf blades of the stay-green type, as compared with leaf blades of the conventional Anjou 258 hybrid. Concentration of chlorophyll a and a + b was increasing in a linear way in the nitrogen range from 0 to 120 kg N·ha⁻¹, while the amount of chlorophyll expressed in SPAD units ranged from 0 to 150 kg N·ha⁻¹. The stay-green type hybrid showed to be better nourished with nitrogen, on the basis of chlorophyll content in maize leaf blades, in the phase of ear blooming (BBCH 67), in comparison with the conventional hybrid and, at the same time, a lower nitrogen fertilization was needed. A simultaneous fertilization with magnesium increased the content of chloroplast pigments, in comparison with the application of nitrogen only. With the increase in the assimilation area of a single maize plant and of a maize stand (LAI indicator), the chlorophyll concentration in leaf blades was decreasing in a linear way.
Plants absorb light energy for photosynthesis, and some amount of potentially damaging range of solar radiation, ultraviolet B. It accounts for less than 0.5% of the total solar radiation and the ambient current level of UV-B during the growth season provide 10 kJ·m–2 energy per day on the Earth’s surface. In the field conditions, increased UV-B radiation is often accompanied by drought, but negative effect of combined stresses is not so deleterious as the effect of one of them alone. We studied some changes in nitrogen me deficit and UV-B radiation. The stresses generally decreased the biomass and total dry matter production. Combination of water deficit and UV-B activated a stress tolerance mechanism in cucumber seedlings. The NR activity and synthesis of UV-B absorbing compounds were induced. Protection against UV-B radiation can partially involve increased production of UV-B protective pigments – slightly increased the SPAD value in cucumber cotyledons and leaves was observed. The deleterious effect of combined stresses was weaker than their additive or individual effects.
Analyses were conducted on leaves of two ×Heucherella L. cultivars: ‘Solar Power’ and ‘Kimono’. Leaves were harvested at two dates: spring (2014-05-22) and summer (2014-07-26). Prior to each leaf harvest day, the maternal plants were sprayed with benzyladenine (BA) at a concentration of 100, 300 and 600 mg dm−3. After being cut, the leaves were placed in containers with distilled water. In the cultivar ‘Solar Power’, BA at a concentration of 100–600 mg dm−3 had an advantageous effect on post-harvest longevity of leaves harvested in summer, improving it by 31.7–98.3%, while in the cultivar ‘Kimono’ BA at the above-mentioned concentrations improved post-harvest longevity of leaves harvested both in spring and summer by almost 2 months. BA at a concentration of 100–600 mg dm−3 inhibited proteolysis in leaves of the tested ×Heucherella cultivars, except for leaves of the cultivars ‘Solar Power’ and ‘Kimono’ harvested in summer from plants sprayed with BA at a concentration of 100 mg dm−3. In these leaves, the protein level was comparable to that in the control leaves. In leaves treated with BA at a concentration of 100–600 mg dm−3, the content of saccharides was higher than in the control plants harvested in the spring and summer.
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