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The studies conducted in 2010–2011 at The Research Institute of Horticulture in Skierniewice were aimed at comparing the effectiveness of different weed management methods in outdoor grown pepper. The following methods were compared: application of herbicide, a combination of herbicide with mechanical treatment, mechanical treatments, the usage of mulches (polypropylene, woven and biodegradable films) and hand weeding. Weed control effect, the number and biomass of weeds, secondary weed infestation, plant height and the yield of pepper were determined during the experiments. The ecophysiological indexes such as relative variable chlorophyll fluorescence and chlorophyll content in the leaves were also calculated. The best results of weed controlling were achieved in pepper weeded by hand and grown in the soil mulched with polypropylene and woven films. The highest yield of fruits was obtained from pepper weeded by hand, treated with herbicide and mulched with woven foil, while the lowest yield was obtained from plants mulched with biodegradable foil. At the beginning of growing season the chlorophyll content in the leaves was not changed in pepper treated with herbicide and hand weeded but its decrease was recorded in pepper grown in mulches. The relative variable chlorophyll fluorescence was the highest in hand weeded pepper at the whole growing season.
Experiments were carried out in 2007–2008, to determine the residues of linuron and flurochloridone in celeriac grown by different cultivation method, using cover crops. Field experiments were performed at the Institute of Vegetable Crops in Skierniewice and analysis of residues at the Institute of Soil Science and Plant Cultivation. Celeriac was grown in cover crops cut and incorporated with the soil, in mulch from cover crops and without cover crops. Linuron at the rate of 0.9 kg/ha and flurochloridone – 0.75 kg/ha, were applied after celeriac planting. The samples of leaves were taken 29, 91 and 125 days after treatment (DAT), root 91 and 125 days and soil samples 18, 29, 88, 91 and 125 DAT. The residues of linuron were determined by liquid chromatography (HPLC) with UV detection and flurochloridone by the method of gas chromatography with EC detector. The residue of linuron and flurochloridone exceeded the acceptable level at 29 DAT, only. Method of cultivation influenced the residue level – the highest level was achieved in cover crops cut and incorporated with the soil.
The studies were conducted in 2010–2011 at the Research Institute of Horticulture in Skierniewice. The aim of the research was to determine the nature and degree of damages and histological changes in celeriac caused by herbicides that are not recommended for application in this crop, such as: bromoxynil, clopyralid, fluroxypyr, MCPA and tribenuron methyl. The herbicides were applied 2–3 weeks after transplanting in the field experiments and in the greenhouse tests at 3–4 leaves stage of celeriac. The herbicides were used at the maximum recommended doses allowed in cultivation of other plant species or reduced to 10 and 2%. Phytotoxicity of herbicides to celeriac was determined during the experiments. The height and biomass of celeriac leaves were measured in the greenhouse tests. Plant material, which had been collected from the experiments, was subjected to macroscopic observation regarding morphological changes of leaves, and microscope evaluation of damages caused to leaf tissues. It has been proved that the examined herbicides can cause damages to celeriac and degree of damages depends on herbicide dose. Clopyralid, fluroxypyr and tribenuron methyl used at the highest doses caused higher crop damages than bromoxynil and MCPA. The symptoms were also observed during histological analysis. High damages of above ground parts of celeriac decreased yields in particular after application of tribenuron methyl and fluroxypyr.
The aim of the study was to determine the response of weeds and drilled onion to pendimethalin CS (Stomp Aqua 455 CS), a new formulation for vegetable crops in Poland. Experiments were carried out in 2009–2010 at the Research Institute of Vegetable Crops at Skierniewice and in Głuchów Dolny. Stomp Aqua 455 CS was applied at the dose of 2.5–3.5 l/ha and the results were compared to the standard EC formulation (Stomp 330 EC – 3 l/ha). Onion was treated by herbicides directly after sowing and post emergence, at 1–2 true leaves stage. It was proved a good efficacy of tested formulation of pendimethalin in each experiment. All tested formulations of pendimethalin were selective to drilled onion in both dates of application. The yield of onion increased after herbicides application, and the differences were significant, as compared to the control object.
The studies have been conducted in 2010–2011 at The Research Institute of Horticulture in Skierniewice. The aim of the research was to determine the yield of pods and the storage ability and nutritional value of snap bean, depending on methods of weed control. In field experiments the following methods were compared: use of herbicides, herbicide + mechanical treatment, mechanical treatments, soil mulching with polypropylene (PP) and hand weeding. The yield of snap bean was evaluated after harvest and after the storage. Snap been pods were storaged at 8°C for 8 days. Chemical analyses were performed twice, immediately after harvest and after the storage period. These analyses covered: dry matter, ascorbic acid, simple and total sugars and nitrates content. Additionally hardness and colour indexes of snap bean pods were determined. The yield of snap bean after harvest was affected by weed management methods. The best storage ability was found for snap bean cultivated in soil mulched with PP foil. The decrease of ascorbic acid content in stored snap bean was found but it was not affected by method of weed control. A higher level of nitrates in snap bean after harvest was recorded after mechanical treatment as compared to hand weeding.
Experiments were carried out in 2005–2007 in the Research Institute of Vegetable Crops at Skierniewice to evaluate the effectiveness of weed control by using metribuzin applied with microrates method. Metribuzin applied in 4–6 treatments at the doses of 36 g/ha after emergences of carrot was effective in weed control. Application of this herbicide at very low doses allowed to reduce the amount of herbicide by aproximately 25–50%. Metribuzin was caused phytotoxicity symptoms on carrot plants but no permanent damages to the crop was stated. The yield of carrot roots increased after applying metribuzin with micro-rate method. Highly significant correlation (R2 = 0.998) showed that with increasing of weed control, the higher yield of carrot roots was obtained.
Field experiments were carried out in 2010–2012 at the Research Institute of Horticulture in Skierniewice. The aim of this studies was to determine the response of drilled onion and weeds to herbicides combined with mechanical treatments, in comparison to the usages of herbicides and the impact of weed management methods on the weed infestation and the yield of onion. During the experiments the following herbicides were used: pendimethalin applied after sowing of onion or after emergences in mixture with bromoxynil, glyphosate pre-emergence and bromoxynil and oxyfluorofen post-emergence. Mechanical treatments were conducted at 2–4 and 3–5 leaves stage of onion. Studies shown that weed control depended on herbicides and their rates. Mechanical treatments performed after herbicides application caused strong reduction of infestation and advantageously influenced on the yield of onion. The highest yields were obtained from plots with regular hand weeded and after chemical weed control including the use of pendimethalin after sowing, glyphosate pre-emergence and bromoxynil post-emergence, with the split application method.
The studies were conducted in 2010–2011 at the Research Institute of Horticulture in Skierniewice. The aim of the research was to determine the impact of separate and combined application of biostimulants Asahi SL and AlfaMax, adjuvants Olbras 88 EC and Protector on efficacy of metribuzin and linuron and the growth and yield of carrot. Herbicides were used with split-application method. First treatment with metribuzin and linuron was applied at 1 leaf stage of carrot, the second treatment of linuron was performed 10–14 days after the first one, and metribuzin was applied at 2–3 leaves stage of carrot. Asahi SL, AlfaMax, Olbras 88 EC and Protector were applied in mixture with herbicides or separate – 1 day before or 1 day after herbicide treatment. The weed control, number and biomass of weeds, phytotoxicity of tested herbicides and the yield of carrot roots were determined during the experiments. Better weed control was achieved with metribuzin than linuron and the addition of biostimulants and adjuvants did not affect its effectiveness. The addition of adjuvants increased the efficacy of linuron. Applied substances did not increase the phytotoxicity of herbicides to carrot plants. The highest yield was obtained by using a mixture of metribuzin with Asahi SL and Protector at 1 day before metribuzin application.
The studies were conducted in 2010–2012 at the Research Institute of Horticulture in Skierniewice. The aim of the research was to determine the yield of celeriac, its storage ability and nutritional value, depending on weed management methods. In the field experiments the following methods were compared: the use of herbicides, herbicide + mechanical treatment, mechanical treatments, soil mulching with polypropylene and biodegradable foil, soil mulching with polypropylene + biostymulant AlgaminoPlant and hand weeding. After harvest celeriac roots were stored at the temperature 0°C for 213–216 days and after storage were sorted out into the following groups: healthy roots without symptoms of re-growing, healthy roots with symptoms of re-growing, roots with diseases and rotting symptoms. The natural weigh loss was also determined. Chemical analyses were performed after harvest and storage period. These analyses consisted of evaluation of dry matter, ascorbic acid, total sugars and soluble phenols content. The highest yield after the storage period was obtained from plants treated with linuron and additionally mechanically weeded and the lowest from not weeded. After the storage the lower contents of dry matter, in comparison to stored celeriac, were determined. Ascorbic acid contents in celeriac grown in biodegradable foil and weeded by hand was higher than in other methods. The contents of soluble phenols after storage was higher than after harvest, except celeriac treated with flurochloridone.
Before being put out onto the market many fish species sold around the world need to be processed, which may result in the subsequent removal of characteristics used for their classification (head, fins, internal organs). The biochemical characterization of fish species could be achieved using proteins or DNA sequences as species-specific markers. However, since different fish products undergo different processes, the method of analysis has to be chosen according to the modifications undergone by fish constituents during processing. As DNA molecules are more resistant than proteins to various processes (including thermal treatment), DNA analysis appears to be a promising method for fish species identification. For the species identification of the Common Carp (Cyprinus carpio) among 15 different freshwater fish species a specific predesigned molecular - genetic marker of Common Carp (Cyprinus carpio) was used, which comes from the mtDNA control D - loop area. Next we analyzed the presence of mtDNA in DNA isolates of the 15 kinds of freshwater fish and compared them with the Common Carp markers by using the following two PCR identification methods. The isolates were diluted to 10 % concentration, using the TaqMan Real-Time PCR method and the SYBR® Green Real-Time PCR method. The results of using the optimized the SYBR® Green Real-Time PCR method for species identification of the Common Carp (C. carpio) pointed to its suitability. We were able to create an analysis of the monitored standard curve which represented the PCR positive control (C. carpio), containing the characteristic melting peak (up to the melting point 80.72 °C). A single peak indicated a single product (C. carpio) which can be verified upon characterization of the PCR product by agarose gel electrophoresis. The TaqMan Real-Time PCR method with a TaqMan probe is a very sensitive and reliable method of authentication used on food of animal origin. The suitability of this method, which we used for species identification of the Common Carp (C. carpio), was confirmed. Thanks to using this method, already in the 17th cycle of the PCR amplification procedure, the presence of the Common Carp gene (C. carpio) was detected in the positive control and not detected in the rest of the fish samples.
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