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Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury

100%

Liang Y., Yang N., Pan G., Jin B., Ji W.

Cellular and Molecular Biology Letters

2018

tom 23

Background: Pulmonary inflammation and endothelial barrier permeability increase in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) induced by pro-inflammatory cytokines and matrix metalloproteinases (MMPs). However, the relationship between pro-inflammatory cytokines and MMPs in ALI/ARDS remains poorly understood. Methods: A lipopolysaccharide (LPS)-induced ALI rat model was established through intratracheal instillation. The wet/dry ratios of lung tissues were measured, and bronchoalveolar lavage fluid (BALF) was collected to test protein concentrations, total cell/macrophage numbers, and pro-inflammatory cytokine levels. LPS-treated alveolar macrophages were utilized in in vitro experiments. The expression and secretion of MMPs were respectively detected using quantitative PCR, Western blotting and ELISA assays. Results: The levels of IL-33 and MMP2/9 in BALF increased in all the ALI rats with severe lung injury. LPS-induced IL-33 autocrine upregulated the expression of MMP2 and MMP9 through activating STAT3. Neutralizing IL-33 in culture medium with specific antibodies suppressed the expression and secretion of MMP2 and MMP9 in LPS-treated alveolar macrophages. Consistently, eliminating IL-33 decreased the levels of MMP2 and MMP9 in BALF and alleviated lung injury in ALI rats. Conclusion: The IL-33/STAT3/MMP2/9 regulatory pathway is activated in alveolar macrophages during acute lung injury, which may exacerbate the pulmonary inflammation.

The intrabody targeting of hTERT attenuates the immortality of cancer cells

86%

Zhu X., Yang N., Cai J., Yang G., Liang S., Ren D.

Cellular and Molecular Biology Letters

2010

tom 15

nr 1

32-45

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity

72%

Liu D., Chen X.-Y., Xiong R.-P., Ning Y.-L., Li P., Peng Y., Liu P., Zhao Y., Yang N., Zhou Y.-G.

Acta Biochimica Polonica

2012

tom 59

nr 4

Objective. To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism. Methods. The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated. Results. DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX. Conclusions. DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

1 Acta Biochimica Polonica

3 Yang N.

1 Cai J.

1 Chen X.-Y.

1 Ji W.

1 Jin B.

1 Li P.

1 Liang S.

1 Liang Y.

1 Liu D.

1 Liu P.

1 Ning Y.-L.

1 Pan G.

1 Peng Y.

1 Ren D.

1 Xiong R.-P.

1 Yang G.

1 Zhao Y.

1 Zhou Y.-G.

1 Zhu X.

1 2018

1 2012

1 2010

Wyniki wyszukiwania

Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury

The intrabody targeting of hTERT attenuates the immortality of cancer cells

Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity