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The effects of 96-hour exposure to 0.25, 0.125 and 0.06 portions of the 96-hour LC₅₀ of Cu, Zn singly and Cu + Zn mixture on biological (survival, gill ventilation frequency, gill-somatic index) parameters of rainbow trout were studied depending on the stage of development of the fish. Hatching embryos had the greatest sensitivity to the affect of metals singly and in admixture (the highest percentage of mortality). Marked changes were recorded in gill ventilation frequency during exposure to these metals singly and in admixture, which corresponded to changes in their gill-somatic indices. Changes to certain parameters studied (mortality of embryos, especially hatching embryos, gill-somatic index of juveniles) indicated enhanced toxicity of the metal mixture to rainbow trout in ontogenesis.
Our study investigated the behavioural responses of medicinal leech and rainbow trout at different ontogenetic levels under the effect of crude oil (CO) and heavy fuel oil (HFO), performed comparative analysis of the sensitivity of animal responses, evaluated the specificity of these responses, and determined “safe“ toxicant concentrations. Comparison of sensitivity of behavioural responses of leech and fish revealed that the most sensitive response to long-term exposure to CO was leech locomotor activity, while the most sensitive parameter to HFO was the coughing rate in juvenile fish. Our study showed that the sensitivity and specificity of behavioural responses of aquatic animals at different phylogenetic and ontogenetic levels can be successfully used to evaluate the toxicity of ambient water polluted with oil hydrocarbons.
The dynamics of the biochemical parameters of the external mucus of carp Cyprinus carpio L. exposed to 1 μM concentrations of heavy metal salts (CuSO₄·5H₂O Pb(NO₃)₂ and ZnSO₄·7H₂O) were studied. The biochemical parameters of external mucus (specific weight, pH, total protein, hemoglobin, ketones) were measured during exposure at 1, 3, 6, 24 h and regularly during a 21-day post-exposure period. Significant changes in hemoglobin and total protein concentrations were determined during the period of exposure to heavy metals and after it. Correlations of changes in biochemical parameters of mucus with the intensity of some parameters of sun activity and radio radiation flow were found during the post-exposure period. It was concluded that it is necessary to assess the possible complex effects of various origin and the magnitude of environmental factors on fish.
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