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1

Rola fitoaleksyn w naturalnej odporności roślin

100%
Szakiel A.
Postępy Biochemii
|
1991
|
tom 37
|
nr 2
2

Analysis of gypsogenin saponins in homeopathic tinctures

72%
Szakiel A., Henry M.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 4
853-856
A relatively simple and short procedure for the quantitative determination of gypsogenin saponins was performed to evaluate homeopathic tinctures in which those compounds can be regarded as one of the active constituents. This method comprises partial hydrolysis of saponins, subsequent extraction of liberated prosaponin (gypsogenin 3-O-glucuronide) and its analysis by high performance liquid chromatography. Glycyrrhizic acid was used as an internal standard. This method was successfully applied to the analysis of mother tinctures obtained from Saponaria officinalis. Thus, the determination of triterpenoid saponins can be used as a convenient and sufficient method of standardization of selected homeopathic tinctures
3

Antioxidant potency of lyoniside isolated from stems and rhizomes of Vaccinium myrtillus L.

72%
Szakiel A., Henry M.
Acta Societatis Botanicorum Poloniae
|
2010
|
tom 79
|
nr Suppl.1
4

Occurrence of triterpene acids and their derivatives in the soil of native and cultivated habitats of cowberry Vaccininum vitis-idaed L.

72%
Szakiel A., Choluj A.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
5

Distribution of triterpene acids and their derivatives in organs of cow-berry [Vaccinium vitis-idaea L.] plant

72%
Szakiel A., Mroczek A.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 4
733-740
Wild berries of the genus Vaccinium have become increasingly popular in human health promotion due to their nutritional and medicinal properties. Some striking divergence of opinion about the content of triterpenoids in these plants still exists, meanwhile, this very large class of natural isoprenoids exhibits a wide range of biological activities and hence is of growing research interest. An investigation of triterpenoidal constituents from the cowberry (Vaccinium vitis-idaeaL.) plant led to the isolation of two isomeric acids:oleanolic and ursolic and the occurrence of their derivatives in this plant was demonstrated for the first time. Free triterpene acids as well as small amounts of their bound forms (presumable glycosides and glycoside esters) occur in fruits and the vegetative part of the plant, however, in various amounts and different ratios. The total content of both acids was the highest in organs regarded as traditional herbal resources, namely fruits and leaves (1 and 0.6% of dry mass, respectively), whereas it was markedly lower in stems and rhizomes. However, the rhizomes were in turn the plant organ containing relatively the highest amount of the bound forms of both acids (0.01% of dry mass). Ursolic acid was dominant in the whole plant, but the ratio of oleanolic to ursolic acid was significantly different in individual organs, decreasing from the upper (fruits 1:2.4, leaves 1:2) to the lower (stems 1:3.5, rhizomes 1:5.2) parts of the plant. This pattern of distribution of triterpenoids in the plant may have an important physiological and ecological meaning.
6

The effect of pH and ATP on the transport of oleanolic acid monoglycosides into isolatedvacuoles of Calendula officinalis leaves

72%
Szakiel A., Janiszowska W.
Acta Biochimica Polonica
|
1991
|
tom 38
|
nr 1
7

Reversibility of the oleanolic acid monoglycosides transport across the tonoplast in vacuoles isolated from Calendula officinalis leaves

72%
Szakiel A., Janiszowska W.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 1
The possibility of the reversible tonoplast transport of oleanolic acid mono­glycosides was investigated in vacuoles isolated from Calendula officinalis leaf protoplasts. The obtained results point to the reversibility of the transport of monoglucoside I, whereas monoglucuronide F seems to be definitely stored in the vacuolar space.
8

Specificity of the tonoplast transport of the oleanolic acid monoglycosides in the vacuoles from Calendula officinalis leaves

72%
Szakiel A., Janiszowska W.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 2
The specificity of two separate tonoplast permeases transporting oleanolic acid glycosides was investigated in vacuoles isolated from leaf protoplasts of marigold (Calendula officinalis) with the use of chemically synthesized analogs. The results indicate that the proper structure of both parts of oleanolic acid monoglycoside, i.e. aglycon and the sugar moiety, are required for binding to a specific tonoplast carrier.
9

Competition between oleanolic acid glycosides in their transport to isolated vacuoles from Calendula officinalis leaf protoplasts

72%
Szakiel A., Janiszowska W.
Acta Biochimica Polonica
|
1992
|
tom 39
|
nr 1
10

The effect of inorganic pyrophosphate on the transport of oleanolic acid monoglycosides into vacuoles isolated from Calendula officinalis leaves

72%
Szakiel A., Janiszowska W.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 3
The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the the monoglucuronide is passive, and that of the monoglucoside active. The active transport of the monoglucoside is dependent on tonoplast energization created as a result of cooperation of two vacuolar proton pumps: H+-ATPase and H+-PPase.
11

The kinetics of transport of oleanolic acid monoglycosides into vacuoles isolated from Calendula officinalis leaf protoplasts

72%
Szakiel A., Janiszowska W.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 1
12

Comparison of the content of triterpene acids in edible berries of three wild Vaccinium species occurring in Poland

58%
Szakiel A., Choluj A., Paczkowski C.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
13
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Triterpenoid profile of fruit and leaf cuticular waxes of edible honeysuckle Lonicera caerulea var. kamtschatica

58%
Becker R., Paczkowski C., Szakiel A.
Acta Societatis Botanicorum Poloniae
|
2017
|
tom 86
|
nr 1
Edible honeysuckle (honeyberry) Lonicera caerulea is becoming popular as a novel berry crop with several useful features such as early fruit ripening and exceptional hardiness, particularly resistance to pests and diseases as well as severe frosts in winter and droughts in summer. The triterpenoid profile of fruit and leaf cutic­ular waxes of edible honeysuckle (a Russian cultivar Chernichka) was analyzed by GC-MS. The major compounds identified were the tetracyclic triterpenoids campesterol, cholesterol, cycloartanol, cycloart-23-ene-3,25-diol, 24-methylenecy­cloartanol (only in leaves), sitosterol, stigmasta-3,5-dien-7-one, and stigmasterol; and the pentacyclic triterpenes: α-amyrin, β-amyrin, hop-22(29)-en-3-one, olea­nolic acid, and ursolic acid. Several remarkable features of the analyzed triterpenoid contents were revealed, including the relatively low abundance of triterpenoids in fruit waxes (6.5% of wax extract) compared to leaf waxes (22%), and a particularly high proportion of tetracyclic triterpenoids (tetracyclic to pentacyclic compound ratios of 4:1 in fruits and almost 7:1 in leaves). These rare features distinguish the triterpenoid profile of the cuticular waxes of L. caerulea var. kamtschatica from the majority of triterpenoid profiles in plant cuticular waxes investigated to date. To our knowledge, this is the first quantitative compositional study on triterpe­noid compounds in the cuticular waxes of edible honeysuckle, supplementing the knowledge of cuticular triterpenoid diversity and distribution.
14

Metabolism of [3-3H]oleanolic acid in Calendula officinalis L. roots

58%
Ruszkowski D., Szakiel A., Janiszowska W.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 4
15

Excretion of oleanolic acid glycosides to the medium from the roots of marigold [Calendula officinalis L.]

58%
Szakiel A., Ruszkowski D., Janiszewska W.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
16

Modifications of pectin methylesterases activity and leaf growth during acclimation and deacclimation of oilseed rape plants

58%
Solecka D., Szakiel A., Daciuk V., Kacperska A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
17

Metabolism of [3-3H]oleanolic acid in the isolated Calendula officinalis leaf cells and transport of the synthesized glycosides to the cell wall and the extracellular space

58%
Szakiel A., Wasiukiewicz I., Janiszowska W.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 1
It has been shown for the first time that [3-3H]oIeanolic acid glycosides formed in the cytosol of C. officinalis leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-3H]oleanolic acid 3-O-monoglucuronide (29%) as well asa part of glucosides (24%) were transported into the cell walls.
18
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Hairy root cultures of marigold (Calendula officinalis ) as a source of oleanolic acid glycosides

51%
Markowski M., Alsoufi A. S. M., Paczkowski C., Szakiel A., Dlugosz M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
19
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Content available

Heligmosomoides polygyrus: rozwoj wolno-zyjacych stadiow hamowany przez roslinne triterpenoidy pentacykliczne

37%
Doligalska M., Grzelak M., Laskowska M., Donskow-Schmelter K., Choluj A., Wiktorowska E., Szakiel A., Janiszowska W.
Wiadomości Parazytologiczne. Suplement
|
2007
|
tom 53
41
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