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Growth characteristics of Fusarium spp. causing wilt disease in Psidium guajava L. in India

100%

Gupta V. K., Misra A. K., Gaur R. K.

Journal of Plant Protection Research

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2010

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tom 50

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nr 4

Fusarium oxysporum f. sp. psidii and F. solani, causal agents of wilt in guava are highly variable pathogens. This study was conducted on cultural and physiological (temperature and pH) characters. The data revealed that maximum mycelial growth was obtained in potato dextrose agar as semi-solid media i.e. 78.00 mm for F. oxysporum f. sp. psidii; 73. 83 mm for F. solani, while malt extract broth as liquid broth media i.e. 1 385 mg mycelia for F. oxysporum f. sp. psidii; 1491 mg for F. solani. Maximum sporulation was recorded in oatmeal agar and mycological broth. The optimum temperature and pH for growth of both Fusarium spp. isolates was 28°C and 5.5. The isolates differed in their colony growth; mycelial mass, macro-conidia, and micro-conidia produced. These variations were characters of each of the isolates with respect to cultural and physiological characters.

2

RAPD-PCR of Trichoderma isolates and in vitro antagonism against Fusarium wilt pathogens of Psidium guajava L.

88%

Gupta V. K., Misra A. K., Gupta A., Pandey B. K., Gaur R. K.

Journal of Plant Protection Research

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2010

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tom 50

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nr 3

The paper concerns randomly amplified polymorphic DNA RAPD-PCR analysis of seven Trichoderma speciesisolates and their in vitro antagonism against wilt pathogens of Psidium guajava L. viz. Fusarium oxysporum f. sp. psidii (F. o.f. sp. psidii) and Fusarium solani. Out of 10 RAPD oligonucleotides (OPA 1-OPA 10) tested, seven markers OPA 1, 3, 5, 7, 8, 9 and10 efficiently differentiated the isolates of Trichoderma and showed reproducible banding patterns. A total of 248 bands were obtained from these markers along with a 61.84% per cent similarity among the seven isolates of Trichoderma.From the seven isolates of Trichoderma, isolates T. harzianum, T.virens and T. viride were evaluated for in vitro efficacy against F. o. f. sp. psidii and F. solani. Per cent inhibition was maximum by direct use of Trichoderma spp. in dual cultures against both pathogens, F. o. f. sp. psidii and F.solani. Two species, T. virens and T. viride were superior in inhibiting the growth of both Fusarium spp. Fusarium isolates showed intra species variability.

3

Distribution and identification of endophytic Streptomyces species from Schima wallichii as potential biocontrol agents against fungal plant pathogens

88%

Passari A. K., Mishra V. K., Gupta V. K., Saikia R., Singh B. P.

Polish Journal of Microbiology

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2016

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tom 65

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nr 3

The prospective of endophytic microorganisms allied with medicinal plants is disproportionally large compared to those in other biomes. The use of antagonistic microorganisms to control devastating fungal pathogens is an attractive and eco-friendly substitute for chemical pesticides. Many species of actinomycetes, especially the genus Streptomyces, are well known as biocontrol agents. We investigated the culturable community composition and biological control ability of endophytic Streptomyces sp. associated with an ethanobotanical plant Schima wallichi. A total of 22 actinobacterial strains were isolated from different organs of selected medicinal plants and screened for their biocontrol ability against seven fungal phytopathogens. Seven isolates showed significant inhibition activity against most of the selected pathogens. Their identification based on 16S rRNA gene sequence analysis, strongly indicated that all strains belonged to the genus Streptomyces. An endophytic strain BPSAC70 isolated from root tissues showed highest percentage of inhibition (98.3 %) against Fusarium culmorum with significant activity against other tested fungal pathogens. Phylogenetic analysis based on 16S rRNA gene sequences revealed that all seven strains shared 100 % similarity with the genus Streptomyces. In addition, the isolates were subjected to the amplification of antimicrobial genes encoding polyketide synthase type I (PKS-I) and nonribosomal peptide synthetase (NRPS) and found to be present in most of the potent strains. Our results identified some potential endophytic Streptomyces species having antagonistic activity against multiple fungal phytopathogens that could be used as an effective biocontrol agent against pathogenic fungi.

4

A kidney bean trypsin inhibitor with an insecticidal potential against Helicoverpa armigera and Spodoptera litura

88%

Mittal A., Kansal R., Kalia V., Tripathi M., Gupta V. K.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 02

In the present study, trypsin inhibitor extracts of ten kidney bean seed (Phaseolus vulgaris) varieties exhibiting trypsin and gut trypsin-like protease inhibitor activity were tested on Helicoverpa armigera and Spodoptera litura. Trypsin inhibitor protein was isolated and purified using multi-step strategy with a recovery of ~15 % and purification fold by ~39.4. SDS-PAGE revealed a single band corresponding to molecular mass of ~15 kDa and inhibitory activity was confirmed by reverse zymogram analyses. The inhibitor retained its inhibitory activity over a broad range of pH (3–11), temperature (40–60°C) and thermostability was promoted by casein, CaCl₂, BSA and sucrose. The purified inhibitor inhibited bovine trypsin in 1:1 molar ratio. Kinetic studies showed that the protein is a competitive inhibitor with an equilibrium dissociation constant of 1.85 μM. The purified trypsin inhibitor protein was further incorporated in the artificial diet and fed to second instar larvae. A maximum of 91.7 % inhibition was obtained in H. armigera, while it was moderate in S. litura (29 %) with slight varietal differences. The insect bioassay showed 40 and 22 % decrease in larval growth followed by 3 and 2 days delay in pupation of H. armigera and S. litura, respectively. Some of the adults emerged were deformed and not fully formed. Trypsin inhibitor protein was more effective against H. armigera as it showed 46.7 % mortality during larval growth period compared to S. litura (13.3 %).

5

Purification, characterization and evaluation of insecticidal potential of trypsin inhibitor from mungbean (Vigna radiata L. Wilczek) seeds

88%

Kansal R., Gupta R. N., Koundal K. R., Kuhar K., Gupta V. K.

Acta Physiologiae Plantarum

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2008

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tom 30

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nr 6

Protease inhibitors present in seeds of legumes possess strong inhibitory activity against trypsin and confer resistance against pests. In the present investigation, trypsin inhibitor activity was found in the seed flour extracts of all the eight selected varieties of mungbean under study which was further confirmed by dot blot analysis. All the varieties showed inhibitory activity in vitro against the gut protease of Helicoverpa armigera (HGP). Trypsin inhibitor was purified from mungbean seeds to near homogeneity with 58.1-fold and 22.8% recovery using heat denaturation, NH4(SO4)2 fractionation, ion-exchange chromatography on DEAE-Sephadex A-25 and gel filtration through Sephadex G-75. The molecular mass of the inhibitor was 47 kDa as determined by gel filtration and SDS-PAGE. The inhibitor retained 90% or more activity between pH 4 and 10, however, it was nearly inactive at extreme pH values. The inhibitor was stable up to 80℃ but thereafter, the activity decreased gradually retaining nearly 30% of activity when heated at 100℃ for 20 min. The inhibitor activity was undetectable at 121℃. Insect bioassay experiment using purified mungbean trypsin inhibitor showed a marked decline in survival (%) of larvae with increase in inhibitor concentration. The larval growth was also extended by the trypsin inhibitor. This study signifies the insecticidal potential of mungbean trypsin inhibitor which might be exploited for raising transgenic plants.

6

A Bowman–Birk protease inhibitor with antifeedant and antifungal activity from Dolichos biflorus

76%

Kuhar K., Kansal R., Subrahmanyam B., Koundal K. R., Miglani K., Gupta V. K.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 06

In the present study, 11 varieties of Dolichos biflorus exhibited both protease inhibitor activities as well as in vitro inhibitory activity against Helicoverpa armigera gut protease. A Bowman–Birk protease inhibitor showing activity against trypsin and α-chymotrypsin has been purified from D. biflorus seeds using multi-step strategy. The purified inhibitor revealed a single band on SDSPAGE corresponding to molecular mass of 16 kDa. The inhibitory constants for the interaction of purified PI with trypsin and α-chymotrypsin were 0.04 and 0.48 lM, respectively. The purified inhibitor was stable over a pH range of 2–12 and up to a temperature of 100 ºC for 20 min. The results of insect bioassay against H. armigera revealed 68 % decline in larval weight after 7 days of feeding on artificial diet containing the inhibitor. The larval growth and % leaf area eaten were drastically reduced in the presence of inhibitor. The observed cumulative mortality from larval to adult was 51.21 %. The inhibitor displayed antifungal activity against Alternaria alternata, Fusarium oxysporum, and Aspergillus niger with minimum inhibitory concentration as 0.4, 0.6, and 1.2 μg mL-1, respectively. This is the first report of anti-feedant and anti-fungal activities of D. biflorus protease inhibitor on a single protein, which might be important for developing transgenic plants resistant to insect pests and fungal pathogens.

7

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Comparative evaluation of inversion approaches of the radiative transfer model for estimation of crop biophysical parameters

64%

Mridha N., Sahoo R. N., Sehgal V. K., Krishna G., Pargal S., Pradhan S., Gupta V. K., Nagesh Kumar D.

International Agrophysics

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2015

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tom 29

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nr 2

The inversion of canopy reflectance models is widely used for the retrieval of vegetation properties from remote sensing. This study evaluates the retrieval of soybean biophysical variables of leaf area index, leaf chlorophyll content, canopy chlorophyll content, and equivalent leaf water thickness from proximal reflectance data integrated broad bands corresponding to moderate resolution imaging spectroradiometer, thematic mapper, and linear imaging self scanning sensors through inversion of the canopy radiative transfer model, PROSAIL. Three different inversion approaches namely the look-up table, genetic algorithm, and artificial neural network were used and performances were evaluated. Application of the genetic algorithm for crop parameter retrieval is a new attempt among the variety of optimization problems in remote sensing which have been successfully demonstrated in the present study. Its performance was as good as that of the look-up table approach and the artificial neural network was a poor performer. The general order of estimation accuracy for para-meters irrespective of inversion approaches was leaf area index > canopy chlorophyll content > leaf chlorophyll content > equivalentleaf water thickness. Performance of inversion was comparable for broadband reflectances of all three sensors in the optical region with insignificant differences in estimation accuracy among them.

8

Dietary natural products and their potential to influence health and disease including animal model studies

33%

Kan Yeung A. W., Aggarwal B. B., Barreca D., Battino M., Belwal T., Horbanczuk O. K., Berindan-Neagoe I., Bishayee A., Daglia M., Devkota H. P., Echeverria J., El-Demerdash A., Orhan I. E., Godfrey K. M., Gupta V. K., Horbanczuk J. O., Modlinski J. A., Huber L. A., Huminiecki L., Jozwik A., Marchewka J., Miller M. J. S., Mocan A., Mozos I., Nabavi S. F., Mohammad Nabavi S., Pieczynska M. D., Pittala V., Rengasamy K. R. R., Silva A. S., Sheridan H., Stankiewicz A. M., Strzalkowska N., Sureda A., Tewari D., Weissig V., Zengin G., Atanasov A. G.

Animal Science Papers and Reports

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2018

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tom 36

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nr 4

9

Resveratrol, a popular dietary supplement for human and animal health: Quantitative research literature analysis - a review

27%

Wai Kan Yeunga A., Bhushan Aggarwal B., Erdogan Orhan I., Horbanczuk O. K., Barreca D., Battino M., Belwal T., Bishayee A., Daglia M., Devkota J. P., Echeverria J., El-Demerdash A., Balacheva A., Georgieva M., Godfrey K., Gupta V. K., Horbanczuk J. O., Huminiecki L., Jozwik A., Strzelkowska N., Mocan A., Mozos I., Nabavi S. M., Pajpanova T., Pittala V., Feder-Kubis J., Sampino S., Sanches Silva A., Sheridan H., Sureda A., Tewari D., Wang D., Weissig V., Yang Y., Zengin G., Shanker K., Moosavi M. A., Shah M. A., Kozuharova E., Al-Rimawi F., Durazzo A., Lucarini M., Soutoa E. B., Santini A., Malainer C., Djilianov D., Tancheva L. P., Li H.-B., Gan R.-Y., Tzvetkov N. T., Atanasov A. G.

Animal Science Papers and Reports

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2019

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tom 37

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nr 2

Ograniczanie wyników

Growth characteristics of Fusarium spp. causing wilt disease in Psidium guajava L. in India

RAPD-PCR of Trichoderma isolates and in vitro antagonism against Fusarium wilt pathogens of Psidium guajava L.

Distribution and identification of endophytic Streptomyces species from Schima wallichii as potential biocontrol agents against fungal plant pathogens

A kidney bean trypsin inhibitor with an insecticidal potential against Helicoverpa armigera and Spodoptera litura

Purification, characterization and evaluation of insecticidal potential of trypsin inhibitor from mungbean (Vigna radiata L. Wilczek) seeds

A Bowman–Birk protease inhibitor with antifeedant and antifungal activity from Dolichos biflorus

Comparative evaluation of inversion approaches of the radiative transfer model for estimation of crop biophysical parameters

Dietary natural products and their potential to influence health and disease including animal model studies

Resveratrol, a popular dietary supplement for human and animal health: Quantitative research literature analysis - a review