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Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor alpha and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

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He C.-L., Yi Q., Li Y. F., Yang H., Wang L.

Bulletin of the Veterinary Institute in Puławy

2013

tom 57

nr 3

Mammary epithelial cells (MECs) from Kunming mice were isolated and stimulated in vitro with 10 µg/mL of Escherichia coli lipopolysaccharide (LPS). The release of tumour necrosis factor α (TNF-α) and interleukin-8 (IL-8) into culture supernatants was measured by ELISA. Furthermore, blocking experiments with Toll-like receptor 2 (TLR2) and TLR4 antibodies were performed to verify whether cytokine secretion depended on LPS-induced activation of TLR2 or TLR4. The results revealed that LPS-stimulated mouse MECs significantly secreted TNF-α and IL-8. Blocking of the TLR4 pathway inhibited the secretion of TNF-α and IL-8, while inhibition of LPS-induced TNF-α and IL-8 production was not observed when TLR2 was blocked. Thus, TLR4 can mediate the LPS-induced expression of cytokines such as TNF-α and IL-8 in mouse MECs.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 He C.-L.

1 Li Y. F.

1 Wang L.

1 Yang H.

1 Yi Q.

1 2013

Wyniki wyszukiwania

Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor alpha and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells