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Sex determination in 6 bovis species by duplex PCR

100%

Prashant, Gour D. S., Dubey P. P., Jain A., Gupta S. C., Joshi B. K., Kumar D.

Journal of Applied Genetics

2008

tom 49

nr 4

379-381

Sex determination in domestic animals is of potential value to livestock breeding programs. The aim of this study was to develop a simple and accurate PCR-based sex determination protocol, which can be applicable to 6 major domesticated species of the family Bovidae, viz. Bos frontalis, B. grunniens, B. indiens, Babalus bubalis, Capra hircus, and Ovis aries. In silico analysis was done to identify conserved DNA sequence in the HMG box region of the sex-determining region of the Y-chromosome (SRY gene) across the bovids. Duplex PCR assay, including the SRY gene and the GAPDH housekeeping gene, was optimized by using genomic DNA extracted from blood samples of known sex. It was possible to identify the sex of animals by amplifying both gender-specific (SRY) and autosomal (GAPDH) genes simultaneously in the duplex reaction, with the male yielding two bands and the female one band. The protocol was subjected to a blind test that showed a 100 percent specificity and accuracy, thus it can be used in sex determination in livestock breeding programs.

Ograniczanie wyników

1 Journal of Applied Genetics

1 Dubey P. P.

1 Gour D. S.

1 Gupta S. C.

1 Jain A.

1 Joshi B. K.

1 Kumar D.

1 Prashant

1 2008

Wyniki wyszukiwania

Sex determination in 6 bovis species by duplex PCR