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Multituberculates are the most diverse and best known group of Mesozoic mammals; they also persisted into the Paleogene and became extinct in the Eocene, possibly outcompeted by rodents that have similar morphological and presumably ecological adaptations. Among the Paleogene multituberculates, those that have the largest body sizes belong to taeniolabidoids, which contain several derived species from North America and Asia and some species with uncertain taxonomic positions. Of the known taeniolabidoids, the poorest known taxon is Sphenopsalis nobilis from Mongolia and Inner Mongolia, China, represented previously by a few isolated teeth. Its relationship with other multituberculates thus has remained unclear. Here we report new specimens of Sphenopsalis nobilis collected from the upper Paleocene of the Erlian Basin, Inner Mongolia, China, during a multi-year field effort beginning in 2000. These new specimens document substantial parts of the dental, partial cranial and postcranial morphologies of Sphenopsalis, including the upper and lower incisors, partial premolars, complete upper and lower molars, a partial rostrum, fragments of the skull roof, middle ear cavity, a partial scapula, and partial limb bones. With the new specimens we are able to present a detailed description of Sphenopsalis, comparisons among relevant taeniolabidoids, and brief phylogenetic analyses based on a dataset consisting of 43 taxa and 102 characters. In light of the new evidence, we assess the phylogenetic position of Sphenopsalis and re-establish the family Lambdopsalidae. The monophyly of Taeniolabidoidea is supported in all our phylogenetic analyses. Within Taeniolabidoidea the Asian lambdopsalids and the North American taeniolabidids represent two significantly different trends of adaptations, one characterized by shearing (lambdopsalids) and the other by crushing and grinding (taeniolabidids) in mastication, which supports their wider systematic separation, as speculated when Sphenopsalis was named.
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A new genus and species of Tritylodontidae, Yuanotherium minor, is described and compared with other known tritylodontids. The new taxon is represented by a partially preserved upper jaw with three postcanines, collected from the upper part of the Shishugou Formation (Oxfordian, Late Jurassic) in the Wucaiwan area of the Junggar Basin, northwestern Xinjiang, China. Like other tritylodontids its maxillary teeth have three rows of blade−like trenchant cusps separated by deep furrows. The new species differs from other tritylodontids mainly in having posteriormost two cusps of the median row on upper postcanines closely placed. The new tritylodontid may have been omnivorous rather than herbivorous, as previously suggested for tritylodontids in general.
We describe a partially crushed skull and dentaries of a sub-adult individual of Daulestes nessovi sp. n., from the Coniacian of Uzbekistan. This is the earliest known eutherian skull (about 87 Ma) and the sixth genus of Cretaceous eutherians in which a skull is available. Because the skull of D. nessovi is sub-adult, certain plesiomorphic features may be ontogenetic and should be interpreted with caution. Four upper premolars and five lower premolariform teeth were in use (possibly to become four lowers when fully adult). The upper cheek-teeth have winged conules; M2 has large parastylar and small metastylar projections. Pre- and postcingula are lacking on DP4 and the upper molars. The talonids of dp4-m2 are about 90% as wide as the trigonids, with widely separated entoconid and hypoconulid. The skull has a large sphenorbital fissure, no foramen rotundum, and apparently no pterygoid process of the sphenoid. A large orbital wing of the palatine prevents maxilla-frontal contact within the orbit. The zygomatic arch is slender. The cochlea has one full turn, with an expanded apex, which suggests that a lagena might have been present. A large malleus with a robust anterior process, and a large promontorium may be due to young age of the individual or a primitive retention, as in the platypus. Because of the similarity to Asioryctidaem both cranial structure and dentition, we assign Daulestes tentatively to Asioryctitheria Novacek et al. 1997, family incertae sedis.
The effects of 24-epibrassinolide (EBR) on chlorophyll fluorescence, leaf surface morphology and cellular ultrastructure of grape seedlings (Vitis vinifera L.) under water stress were investigated. The grape seedlings were subjected to 10 % (w/v) polyethylene glycol (PEG-6000) and treated with 0.05, 0.10 or 0.20 mg L⁻¹ EBR, respectively. EBR application increased chlorophyll contents, the effective photochemical quantum yield of PSII, maximum photochemical efficiency of PSII, maximal fluorescence and non-photochemical quenching coefficient under water stress in each concentration. Compared with water stress control, higher stomatal density and stomatal length were observed in young leaves under EBR treatments, but not in mature leaves. In-depth analysis of the ultrastructure of leaves indicated that water stress induced disappearance of nucleus, chloroplast swelling, fractured mitochondrial cristae and disorder of thylakoid arrangement both in young leaves and mature leaves. However, EBR application counteracted the detrimental effects of water stress on the structure of the photosynthetic apparatus better in young leaves than in mature leaves. Compared to the other treatments, treatment of 0.10 mg L⁻¹ EBR had best ameliorative effect against water stress. These results suggested that exogenous EBR could alleviate water stress-induced inhibition of photosynthesis on grape possibly through increasing chlorophyll content, lessening the stomatal and non-stomatal limitation of photosynthesis performance.
Microtoid cricetids are widely considered to be the ancestral form of arvicoline rodents, a successful rodent group includ− ing voles, lemmings and muskrats. The oldest previously known microtoid cricetid is Microtocricetus molassicus from the Late Miocene (MN9, ca. 10–11 Ma) of Europe. Here, we report a new microtoid cricetid, Primoprismus fejfari gen. et sp. nov., from the Junggar Basin of Xinjiang, northwestern China. The rodent assemblage found in association with this specimen indicates a late Early Miocene age, roughly estimated at 18–17 Ma, and thus more than 6 million years older than M. molassicus. While morphological comparisons suggest that the new taxon is most closely related to M. molas− sicus, it differs from the latter in a striking combination of primitive characters, including a lower crown, smaller size, a differentiated posterolophid and hypolophid, a faint anterolophid, the absence of an ectolophid, and the presence of a stylid on the labial border of the tooth. Arid conditions prevailing across the mid−latitude interior of Eurasia during the Early Miocene, enhanced by the combined effects of the Tibetan uplift and the gradual retreat of the Tethys Ocean, likely played a role in the appearance of grasslands, which in turn triggered the evolution of microtoid cricetids and, ultimately, the origin of arvicoline rodents.
In order to reveal the differentiated responses of plankton and zoobenthos to water quality on both annual and seasonal time scales, an ecological study on plankton and zoobenthos community was conducted during 2011 to 2015 in Nansi Lake in northern China. Different dynamics were found among phytoplankton, zooplankton, and zoobenthos in terms of density, biomass, and biodiversity. On an annual time scale and with deterioration of water quality, density and biomass of plankton and zoobenthos showed different variations, while phytoplankton (density, biomass, and biodiversity) changed significantly when water quality improved. On a seasonal time scale, density and biomass of phytoplankton showed significant differences between spring and summer. Results of Pearson’s correlation analysis revealed that phytoplankton, zooplankton, and zoobenthos were significantly affected by different environmental factors. Our study demonstrated that phytoplankton was more sensitive to water quality changes than zooplankton and zoobenthos. These results suggest that density and biomass of phytoplankton could rapidly reflect water quality, whereas biodiversity of phytoplankton could indicate long-term status of water quality combined with the physico-chemical parameters of water. The results could contribute to predicting the dynamics of plankton and zoobenthos in freshwater lakes and to selecting effective bio-indicators for ecosystem health assessment of freshwater lakes.
Controlled irrigation and drainage (CID) has received considerable attention as a reliable management practice for improving water quality and water productivity in rice production. This study aimed to evaluate the effects of CID on water productivity, nitrogen, and phosphorus losses in paddy fields. Treatments include alternate wetting and drying (AWD; lower limit of irrigation to -200 mm and upper limit of ponding water depth after rainfall to 60 mm), CID-I (lower limit of irrigation to -200 mm and upper limit of ponding water depth after rainfall to 200 mm), and CID-II (lower limit of irrigation to -500 mm and upper limit of ponding water depth after rainfall to 200 mm). Results showed that CID reduced irrigation water without a significant impact on grain yields and increased the irrigation water productivity by 14.6-51.5% compared with AWD. However, the percolation of CID may be increased, especially in a wetting year. The application of CID-II by combining yield with irrigation water productivity could be suitable and beneficial to rice crops. The average total nitrogen (TN) and total phosphorus (TP) concentrations of CID presented similar values or were significantly increased relative to AWD, indicating that the significant decreases in nutrient loads under CID were primarily due to reductions in surface runoff rather than changes in concentration. Ammonium nitrogen (NH₄⁺-N) concentrations were clearly increased after fertilizer application in percolation water. Compared with AWD, the NH₄⁺-N, TN, and TP leaching losses of CID-I were increased. The nitrogen and phosphorus leaching losses of CID-II were significantly increased relative to AWD and CID-I because of high nutrient concentrations and severe preferential flow. Therefore, CID potentially increased nitrogen and phosphorus loading to groundwater when the lower limit irrigation was used. The results indicate that the suitable application of CID can save fresh water, reduce nutrient losses, and guarantee rice production.
Heterozygous missense mutations in IHH result in Brachydactyly type A1 (BDA1; OMIM 112500), a condition characterized by the shortening of digits due to hypoplasia/aplasia of the middle phalanx. Indian Hedgehog signaling regulates the proliferation and differentiation of chondrocytes and is essential for endochondral bone formation. Analyses of activated IHH signaling in C3H10T1/2 cells showed that three BDA1-associated mutations (p.E95K, p.D100E and p.E131K) severely impaired the induction of targets such as Ptch1 and Gli1. However, this was not a complete loss of function, suggesting that these mutations may affect the interaction with the receptor PTCH1 or its partners, with an impact on the induction potency. From comparative microarray expression analyses and quantitative real-time PCR, we identified three additional targets, Sostdc1, Penk1 and Igfbp5, which were also severely affected. Penk1 and Igfbp5 were confirmed to be regulated by GLI1, while the induction of Sostdc1 by IHH is independent of GLI1. SOSTDC1 is a BMP antagonist, and altered BMP signaling is known to affect digit formation. The role of Penk1 and Igfbp5 in skeletogenesis is not known. However, we have shown that both Penk1 and Igfbp5 are expressed in the interzone region of the developing joint of mouse digits, providing another link for a role for IHH signaling in the formation of the distal digits.
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