Wyniki wyszukiwania

1

Are there realistic opportunities to apply cloning and the other reproductive biotechnologies for safeguarding endangered species?

100%

Ptak G., Loi P.

Animal Science Papers and Reports. Supplement

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2004

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tom 22

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nr 1

2

Peri- and post-natal mortality of somatic cell clones in sheep

81%

Loi P., Della Salda L., Ptak G.

Animal Science Papers and Reports. Supplement

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2004

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tom 22

|

nr 1

3

Effect of advanced paternal age on ultrasound vocalization in mice

71%

Zacchini F., Sampino S., Swiergiel A., Loi P., Ptak G.

Acta Neurobiologiae Experimentalis

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2011

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tom 71

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nr S

4

The role of cell cycle co-ordination in the development of nuclear transfer reconstructed embryos

71%

Campbell K. H. S., Ritchie W., Loi P., McWhir J., Wilmut I.

Biotechnologia

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1996

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nr 2

5

Transcriptomic changes in fetal brain and placenta associated with advanced paternal age behavioral abnormalities

61%

Sampino S., Stankiewicz A. M., Goscik J., Swiergiel A. H., Loi P., Modlinski J. A., Ptak G. E.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

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nr Supl.

BACKGROUND AND AIMS: Advanced Paternal Age (APA) has been shown to be a significant risk factor for neurodevelopmental psychiatric disorders, such as autism. We have recently shown that mice conceived by old fathers display behavioral abnormalities which resemble key diagnostic symptoms of human autism, including social deficits, communicative defects and repetitive behaviors. The aim of the present study was to evaluate the transcriptomic profiles of brains and placentas collected from fetuses conceived by young (CTR) or old (APA) fathers, in order to reveal molecular mechanisms acting early in life leading to postnatal autistic behaviors. METHODS: Tissues were subjected to genome-wide mRNA expression analysis using Agilent microarray technology and subsequently to Real time qPCR validation. RESULTS: Comparison of fetal brains transcriptomic profiles of CTR and APA offspring revealed that paternal age affected the expression of 1060 genes in males and 857 genes in females. Comparisons of placentas revealed alteration of 3383 genes in males and 711 genes in females. Gene set enrichment analysis performed using i.e. the KEGG pathway database, identified significant functional clusters involved in axonal growth, extracellular matrix receptors, neuroactive ligand receptors and cytokine receptors, in fetal brains. Intriguingly, placental group of deregulated genes represented functional networks involved in neuronal and metabolic pathways. qPCR confirmed that expression of genes involved in axonal growth (Neurod2, Neurod6, Epha5) were deregulated in brains of male fetuses, and that neuronal-related genes (Nrxn3, Hif3a) were expressed by the placenta and deregulated in APA mice. CONCLUSIONS: Overall, these results indicate that early events of brain development could be altered in fetuses conceived by old males, consistent with an indirect influences of the placenta on early neurodevelopment programming, which could underlie the subsequent onset of behavioral alterations.

6

Effects of blastomere biopsy of preimplantation embryos on expression of imprinted genes and anxiety - and depression - like behaviours in mice

61%

Sampino S., Zacchini F., Swiergiel A., Modlinski J., Loi P., Ptak G.

Acta Neurobiologiae Experimentalis

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2011

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tom 71

|

nr S

Prenatal developmental period is a critical window, where any perturbation of embryonic/fetal environment can lead to behavioural abnormalities and diseases in adult life, such as depression and stress disorders. The mechanisms underlying long term effects, induced by early perturbation, are not well described, but an epigenetic origin has been suggested. In this work we wanted to investigate whether and how blastomere biopsy on 8-cells stage embryos can have long-term effects on behaviour. Onemonth-old mice derived from the biopsed embryos were subjected to a battery of behavioural tests. The animals displayed an increased locomotor and exploration activity (p<0.05) and increased anxiety-like behaviour. Interestingly, the depressionlike behaviour in the tail suspension test was observed only in female offspring (p<0.001). In addition, to investigate the epigenetic mechanism underlying these behavioural alterations, we analyzed expression of imprinted genes Snrpn, Peg1 and Ube3a in blastocysts obtained after biopsy. These imprinted genes are highly expressed in pre-implantation embryos, where their epigenetic programming is defined, and in brain, shaping the behavioural phenotype of offspring. Real-Time PCR analysis revealed significant down-regulation of Peg1 (p<0.05), Snrpn and Ube3a (p<0.01) in blastocysts derived from the biopsed embryos, compared to controls. The results suggest that blastomere biopsy causes an altered expression of imprinted genes in preimplantation embryo. The reduction of expression of these transcripts can cause anxiety- or depression-like behaviours and alteration of locomotory activity in offspring obtained following biopsy of early embryos.

7

Effect of advanced paternal age on ultrasound vocalization in mice

61%

Zacchini F., Sampino S., Swiergiel A., Modlinski J., Loi P., Ptak G.

Acta Neurobiologiae Experimentalis

|

2011

|

tom 71

|

nr S

Delayed parenthood is a growing phenomenon in western countries due to modern lifestyle. In particular, delayed fatherhood has been described as a factor of risk for several mental disorders and intermediate phenotypes such as poor cognitive or social functions. In this work we want to investigate whether and how advanced paternal age can affect development of offspring. We evaluated UltraSound Vocalization (USV) activity on 4-day old mice, born from both aged and young (control) father, after removal from their nest. We found that the total number of USV in the 5 minutes of testing was significantly higher in pups from aged mice than control (p<0.002) and that the main difference was present during the first minute (p <0.0004). Also, our data showed that the number of high intensity USV was increased in pups from aged mice (p<0.002). We also evaluated the righting reflex ability of 6 and 10-days-old mice. We did not find any difference in righting reflex ability between groups. All together our data show that delayed fatherhood affect communication skills and anxiety likebehavior but no innate righting reflex ability. In conclusion, advanced paternal age affects the development of offspring and these effects are detectable already in first days of post-natal life and they could be first signs of brain diseases in adult life. Future analysis will be necessary to define the long term effect of advanced paternal age and the mechanism underlying developmental alterations in offspring.

8

Effects of blastomere biopsy of preimplantation embryos on expression of imprinted genes and anxiety - and depression - like behaviours in mice

61%

Sampino S., Zacchini F., Swiergiel A., Modlinski J., Loi P., Ptak G.

Acta Neurobiologiae Experimentalis

|

2011

|

tom 71

|

nr S

Prenatal developmental period is a critical window, where any perturbation of embryonic/fetal environment can lead to behavioural abnormalities and diseases in adult life, such as depression and stress disorders. The mechanisms underlying long term effects, induced by early perturbation, are not well described, but an epigenetic origin has been suggested. In this work we wanted to investigate whether and how blastomere biopsy on 8-cells stage embryos can have long-term effects on behaviour. One-month-old mice derived from the biopsed embryos were subjected to a battery of behavioural tests. The animals displayed an increased locomotor and exploration activity (p<0.05) and increased anxiety-like behaviour. Interestingly, the depression-like behaviour in the tail suspension test was observed only in female offspring (p<0.001). In addition, to investigate the epigenetic mechanism underlying these behavioural alterations, we analyzed expression of imprinted genes Snrpn, Peg1 and Ube3a in blastocysts obtained after biopsy. These imprinted genes are highly expressed in preimplantation embryos, where their epigenetic programming is defined, and in brain, shaping the behavioural phenotype of offspring. Real-Time PCR analysis revealed significant down-regulation of Peg1 (p<0.05), Snrpn and Ube3a (p<0.01) in blastocysts derived from the biopsed embryos, compared to controls. The results suggest that blastomere biopsy causes an altered expression of imprinted genes in preimplantation embryo. The reduction of expression of these transcripts can cause anxiety- or depression-like behaviours and alteration of locomotory activity in offspring obtained following biopsy of early embryos.

9

Improvement of mouse and sheep cloned blastocysts’ quality upon nuclear transfer of BRDT - expressing somatic cells

51%

Kotlarska M., Czernik M., Iuso D., Winiarczyk D., Szyszka-Peczkowicz J., Greda P., Loi P., Modlinski J. A.

Animal Science Papers and Reports

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2019

|

tom 37

|

nr 3

Ograniczanie wyników

Are there realistic opportunities to apply cloning and the other reproductive biotechnologies for safeguarding endangered species?

Peri- and post-natal mortality of somatic cell clones in sheep

Effect of advanced paternal age on ultrasound vocalization in mice

The role of cell cycle co-ordination in the development of nuclear transfer reconstructed embryos

Transcriptomic changes in fetal brain and placenta associated with advanced paternal age behavioral abnormalities

Effects of blastomere biopsy of preimplantation embryos on expression of imprinted genes and anxiety - and depression - like behaviours in mice

Effect of advanced paternal age on ultrasound vocalization in mice

Effects of blastomere biopsy of preimplantation embryos on expression of imprinted genes and anxiety - and depression - like behaviours in mice

Improvement of mouse and sheep cloned blastocysts’ quality upon nuclear transfer of BRDT - expressing somatic cells