Wyniki wyszukiwania

1

Important residue [G46] in erythroid spectrin tetramer formation

100%

Kang J., Song Y., Sevinc A., Fung L. W.-M.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 1

46-54

Spectrin tetramerization is important for the erythrocyte to maintain its unique shape, elasticity and deformability. We used recombinant model proteins to show the importance of one residue (G46) in the erythroid α-spectrin junction region that affects spectrin tetramer formation. The G46 residue in the erythroid spectrin N-terminal junction region is the only residue that differs from that in non-erythroid spectrin. The corresponding residue is R37. We believe that this difference may be, at least in part, responsible for the 15-fold difference in the equilibrium constants of erythroid and non-erythroid tetramer formation. In this study, we replaced the Gly residue with Ala, Arg or Glu residues in an erythroid α-spectrin model protein to give G46A, G46R or G46E, respectively. We found that their association affinities with a β-spectrin model protein were quite different from each other. G46R exhibited a 10-fold increase and G46E exhibited a 16-fold decrease, whereas G46A showed little difference, when compared with the wild type. The thermal and urea denaturation experiments showed insignificant structural change in G46R. Thus, the differences in affinity were due to differences in local, specific interactions, rather than conformational differences in these variants. An intra-helical salt bridge in G46R may stabilize the partial domain single helix in α-spectrin, Helix C’, to allow a more stable helical bundling in the αβ complex in spectrin tetramers. These results not only showed the importance of residue G46 in erythroid α-spectrin, but also provided insights toward the differences in association affinity between erythroid and non-erythroid spectrin to form spectrin tetramers.

2

Effects of livestock exclusion on soil physical and biochemical properties of a desert rangeland

88%

Qin Y., Niu D., Kang J., Zhou Y., Li X.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 6

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Wild food plants and wild edible fungi of Heihe valley ( Qinling Mountains, Shaanxi, central China): herbophilia and indifference to fruits and mushrooms

88%

Kang Y., Luczaj L., Ye S., Zhang S., Kang J.

Acta Societatis Botanicorum Poloniae

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2012

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tom 81

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nr 4

4

Performance evaluation of histone deacetylases in lungs of mice exposed to ovalbumin aerosols

76%

Su X. M., Ren Y., Li M. L., Zhao X., Kong L. F., Kang J.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 2

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology

1 Polish Journal of Environmental Studies

4 Kang J.

1 Fung L. W.-M.

1 Kang Y.

1 Kong L. F.

1 Li M. L.

1 Li X.

1 Luczaj L.

1 Niu D.

1 Qin Y.

1 Ren Y.

1 Sevinc A.

1 Song Y.

1 Su X. M.

1 Ye S.

1 Zhang S.

1 Zhao X.

1 Zhou Y.

1 2018

1 2015

1 2012

1 2010

Important residue [G46] in erythroid spectrin tetramer formation

Effects of livestock exclusion on soil physical and biochemical properties of a desert rangeland

Wild food plants and wild edible fungi of Heihe valley ( Qinling Mountains, Shaanxi, central China): herbophilia and indifference to fruits and mushrooms

Performance evaluation of histone deacetylases in lungs of mice exposed to ovalbumin aerosols