Although many countries now have programs to lower the levels of lead in the environment, human exposure to Pb remains of concern to public health officials worldwide. The mechanisms of lead toxicity are still not fully understood, but recent findings have recognized the significance of the lead-induced impairment of the cell energy metabolism. This review outlines recent hypotheses and evidence on the role of nucleotide purines in erythrocyte metabolism regulation. It also describes the resynthesis and decomposition of purine nucleotides in erythrocytes, lead distribution in blood and its influence on purine conversion pathways and both glycolytic and pentosophosphate pathway enzymes in erythrocytes.
The aim of the work was to examine the morphology of the bone marrow of mice during stimulation with G-CSF. Experimental Balb C mice were daily injected subcutaneously with 250 μg/kg b.w. G-CSF (Neupogen). After 2, 4 and 6 days of the experiment femurs were obtained for morphological study. On day 2 of the mobilization the amount of haematopoietic cells in the bone marrow increased and dilatation of the sinusoids was observed. Only single leukocytes were observed in the lumen of the vessels. There were numerous leukocytes in the lumen of the sinusoids on day 4 of the mobilization. The morphology of the bone marrow on day 6 was similar to that of the control. Mobilization of mice with G-CSF resulted in migration of haematopoietic cells from the bone marrow and the process is most pronounced on day 4.