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The aim of our study was to investigate the effects of some main factors, temperature, moisture condition, and organic matter input on soil organic carbon mineralization based on the method of an incubation experiment carried out at Hongze Lake wetland in Jiangsu province of China. The result showed that mineralization rate dynamics of soil organic carbon conformed to the logarithmic equation. Temperature increase could accelerate mineralization rate and cumulative mineralization amount of soil organic carbon in the wetland soils, and improving flooded soils most sharply. Flooding could boost mineralization rate and the cumulative mineralization amount of soil organic carbon, but inhibit decomposition of organic matter. Organic matter input increased the mineralization rate and cumulative mineralization amount of soil organic carbon in the treated soils, which was mainly due to enhanced activity of soil microorganisms caused by organic matter input. Our results indicated that soil carbon mineralization was a relatively complicated process influenced by various environmental factors. Thus, it is necessary to conduct further studies on the mathod through which other environmental factors affect soil carbon mineralization in order to understand their role in soil carbon emissions of wetland ecosystems.
This study attempted to explore how salt priming affected salt tolerance in sweet sorghum with emphasis on root Na+ uptake. After 10 days of pretreatment with 150 mM NaCl, plants were stressed with 300 mM NaCl. After salt stress for 7 days, dry matter of root and shoot decreased by 58.7 and 69.7 % in non-pretreated plants and by 37.9 and 41.3 % in pretreated plants. Consistently, pretreated plants maintained higher photosynthetic rate during salt stress, suggesting the enhanced tolerance by salt priming. Salt priming enhanced osmotic resistance, as proline and relative water contents in the leaf were higher in pretreated plants under salt stress. Salt priming alleviated salt-induced oxidative damage not by improving antioxidant protection due to lower increase in leaf malondialdehyde content and no extra induction on ascorbate peroxidase, catalase, superoxide dismutase,ascorbic acid and reduced glutathione in pretreated plants. After 7 days of salt stress, root Na+ efflux increased by 8.5- and 3.9-folds in pretreated and non-pretreated plants, suggesting that salt priming reduced root Na+ uptake, and then root and leaf Na+ accumulation were mitigated in pretreated plants. However, root Na+ extrusion became indifferent between pretreated and non-pretreated plants under salt stress after inhibiting plasma membrane (PM) Na+/H+ antiporter. Thus, the greater Na+ extrusion induced by salt priming had relation to PM Na+/H+ antiporter. Overall, salt priming improved salt tolerance in sweet sorghum by enhancing osmotic resistance and reducing root Na+ uptake.
Based on the data of annual average values of PM₁₀ concentrations in China, this study empirically investigates the spatial autocorrelation of haze pollution in China and the mutual influence of energy consumption and foreign direct investment on haze pollution in China from 2004 to 2014 using the spatial econometric method. Moran’s I values are all above 0 during the 10 years, which indicates that haze pollution in China exists with significant spatial autocorrelation. Then the spatial econometric model estimation results show that energy consumption has a significant and positive effect on haze pollution in China while foreign direct investment has a significant and negative effect on haze pollution. Meanwhile, the regression coefficient of mutual variable of energy consumption and foreign direct investment is 0.063 at the 5% level, which suggests that foreign direct investment plays an important role in regulating the relationship between energy consumption and haze pollution, namely that the aggravation effect of energy consumption on haze pollution will increase with the increase of foreign direct investment. Finally, we provide some policy guidance for controlling haze pollution in China.
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Apical NAplus-Hplus exchangers in the mammalian gastrointestinal tract

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The Slc9a family of nine Na+/H+ exchangers (NHE) plays a critical role in neutral sodium absorption in the mammalian intestine as well as other absorptive and secretory epithelia of digestive organs. These transport proteins mediate the electroneutral exchange of Na+ and H+ and are crucial in a variety of physiological processes, including the fine tuning of intracellular pH, cell volume control and systemic electrolyte, acid-base and fluid volume homeostasis. Here, we review the role of the Na+/H+ exchange mechanism as it relates to the physiology of organs and cells involved in nutrient absorption, and we describe physiological and molecular aspects of individual isoforms, including their structure, tissue-, and subcellular distribution, as well as their regulation by physiological stimuli at the transcriptional and post-transcriptional levels. A particular emphasis is placed on Na+/H+ exchanger isoforms expressed on the apical (brush border) membrane of the epithelial cells, and the consequences of gene-targeted mutation of individual isoforms are discussed in the context of the physiology of digestive organs. Where available, we also provide a review of pathophysiological states related to aberrant expression and/or activity of Na+/H+ exchangers within the confines of the digestive system.
Microcystis aeruginosa is one of the most common blue-green algae species that forms harmful water bloom, which frequently causes serious ecological pollution and poses a health hazard to animals and humans. To understand the progression of algal blooms and to provide a theoretical basis for predicting and preventing the occurrence of algal blooms and reducing the harm of algal bloom to environment, we investigated the diurnal variation of photosynthesis, ATP content and cell division in M. aeruginosa PCC7820. The results showed that the photosynthesis and ATP content of M. aeruginosa PCC7820 exhibited clear circadian rhythm with a period of approximately 24 h and that the periodic rhythms continued for at least three cycles under continuous light conditions. Furthermore, the period length showed that a temperature compensation effect and changes in light cycle or temperature could reset the phase of circadian rhythm. These results indicate that the circadian rhythms of physiological process in M. aeruginosa PCC7820 are controlled by the endogenous circadian clock. Examinations of the number, size and cytokinin content of cells also reveal that the cell division of M. aeruginosa PCC7820 with the generation time of 38.4 h exhibits robust circadian rhythms with a period close to 24 h. The circadian rhythms of cell division may be generated by a biological clock through regulation of the cell division phase of M. aeruginosa PCC7820 via a gating mechanism. The phases in which cell division slows or stop recur with a circadian periodicity of about 24 h.
HAP (a homologue of the ASY/Nogo-B protein), a novel human apoptosis-inducing protein, was found to be identical to RTN3. In an earlier study, we demonstrated that HAP localized exclusively to the endoplasmic reticulum (ER) and that its overexpression could induce cell apoptosis via a depletion of endoplasmic reticulum (ER) Ca2+ stores. In this study, we show that overexpression of HAP causes the activation of caspase-12 and caspase-3. We still detected the collapse of mitochondrial membrane potential (Δωm) and the release of cytochrome c in HAP-overexpressing HeLa cells. All the results indicate that both the mitochondria and the ER are involved in apoptosis caused by HAP overexpression, and suggest that HAP overexpression may initiate an ER overload response (EOR) and bring about the downstream apoptotic events.
TPSI1/Mt4 family genes are a class of genes induced dramatically by Pi starvation, and are involved in Pi allocation in plants. Its members have been isolated from many plant species, but none has been reported in Brassica napus. Here, we isolated two novel members of TPSI1/Mt4 family from B. napus, which were referred to as BnIPS1;1 and BnIPS1;2. The two genes are 592 and 557 bp long, respectively, and share 91 % sequence identity. They are all intronless, and contain numerous short open reading frames and a conserved 22-nucleotide sequence partially complementary to miR399. Expression analysis revealed that the two genes were induced strongly by Pi starvation and weakly by osmotic stress and salinity. The induction of the two genes is rapid and durative in the absence of Pi and is repressible upon Pi resupply. Furthermore, BnIPS1;1 promoter (-1,459/?42 relative to transcription start site) was isolated and fused to reporter gene GUS. The PBnIPS1;1:GUS construct was introduced into Arabidopsis, and intensive GUS staining was observed in Pi-starved plants. These results further our understanding of TPSI1/Mt4 family genes, and demonstrate that BnIPS1;1 and BnIPS1;2 can be used as tool for investigating Pi-starvation signaling in B. napus and BnIPS1; 1 promoter can be used as inducible promoter for cultivating P-efficient crops.
Comparison of the staminate flower and pollen of Betula platyphylla between autotetraploid and diploid individuals may reveal the reason for cross sterility when an autotetraploid male parent is crossed with a diploid female parent. Staminate flowers and pollen from seven autotetraploid individual trees were examined. The morphologies of the staminate flower and pollen and pollen germination of these autotetraploid individuals were compared with diploid trees. The structure of the staminate flower did not differ between autotetraploids and diploids, whereas the size of the staminate flower was larger in the autotetraploids compared with the diploids. The mean diameter and pore depth of the pollen were significantly larger in autotetraploid individuals compared with diploids, and several abnormalities in pollen morphology were found commonly amongst pollen from the autotetraploid trees. The germination rate of pollen from autotetraploids was significantly inferior to that of diploids and there was a significant negative correlation between germination rate and the percentage of abnormal pollen. These results demonstrate that the autotetraploid should not be chosen as the male parent to obtain hybrid triploid progenies because the pollen is abnormal and results in low fertility despite its large size. The results provide important information for selecting and distributing parent strains with the aim of building seed orchards of triploid birch trees.
Climate change is considered one of the greatest threats to biodiversity in future decades. Learning about the habitat preferences and geographical distributions of endangered species is critical for conservation management and planning in the context of climate change. This study investigated the effects of climate change on suitable habitat for Arborophila rufipectus Boulton, an endangered species that is endemic to southwest China. We used the known presence records for this species and a series of environmental variable layers to develop a predictive distribution model using maximum entropy modelling; this model was then used to assess the effects of future climate change on suitable habitat for this species. Our study indicated that climate change might have significant effects on suitable habitat for this species. By 2050, under a no-dispersal hypothesis, more than four-fifths of the habitat currently assessed as suitable would be lost, and the mean latitude of suitable habitat would shift northward by more than 100 kilometres. Based on this model, climate change would also aggravate habitat fragmentation. Under a full-dispersal hypothesis, all four climate trajectories developed by the Canadian Centre for Climate Modelling and Analysis (Ccma) and the Commonwealth Scientific and Industrial Research Organization (CSIRO) present similar trends: the area of suitable habitat is predicted to increase substantially, and habitat fragmentation would be mitigated under the two climate trajectories developed by the Goddard Institute for Space Studies (GISS). Finally, we offer some practical proposals for the future conservation of this endangered species.
Seedlings of Camellia sinensis (L.) were grown hydroponically to study the effect of aluminium (Al) on leaf antioxidant defence system and cell ultrastructure. We found that malondialdehyde (MDA) content decreased at 0–0.32 mM Al, but increased significantly at 0.53 mM Al. Like MDA, hydrogen peroxide (H₂O₂) content increased at 0.53 mM Al; however, no differences were observed at 0–0.32 mM Al. Superoxide dismutase (SOD, EC1.15.1.1) activity remained practically constant at 0–0.32 mM Al, but increased sharply at 0.53 mM Al; catalase (CAT, EC1.11.1.6) and guaiacol peroxidase (GPX, EC1.11.1.7) activities decreased following an initial increase, reaching their peaks at 0.32 mM Al. Ascorbate peroxidase (APX, EC 1.11.1.11) activity increased and glutathione (GR, EC 1.6.4.2) level fluctuated with increasing Al concentrations. Transmission electron microscope analysis of Al-treated leaves showed that although cell ultrastructural integrity was maintained at 0–0.32 mM, significant membrane damage was observed at 0.53 mM. Our results suggest that at low Al concentrations, the leaf antioxidant defence system can scavenge reactive oxygen species and sufficiently protect cells from free radical injury. However, at higher Al concentrations (0.53 mM), the balance between formation and detoxification of ROS is lost, resulting in the destruction of cell ultrastructure.
Water inrush during the excavation of mines or mining is one of the great challenges in underground engineering work in China. To prevent and/or mitigate water inrush, natural sedimentary rocks with low-permeability, which include the mudstone and sandstone found in underground excavations, are exceptional materials for geological barriers. The aim of this work is to therefore investigate the hydraulic properties of sedimentary rocks in a deep tunnel, with the application of high-pressure packer tests (HPPTs). The experimental results indicate that both the mudstone and sandstone are low permeability. The measured injection pressure-flow rate (P-Q) can be divided into two phases: Phase I – a flow that is Darcian with a linear relationship, and Phase II – changes in the flow pattern with an exponential relationship. Water injection primarily triggers fracture dilation, and then results in an evident increase in hydraulic conductivity. The hydraulic conductivity versus water pressure distribution phase can be considered as three flow phases: the initial flow phase, initiation of flow communication phase, and non-steady state flow phase. The calculated original fracture apertures in mudstone and sandstone are, respectively, 0.37 and 0.33 mm. Furthermore, a conceptual model with two phases of fracture aperture versus water pressure is proposed.
Long-term changes (2003-14) of water temperature, DO, pH, TN, NH4-N, TP, and CODMn were examined at eight sampling stations along the Liujiang River. Water quality parameters showed considerable spatial and temporal variability. Annual averages of those parameters were 22.1ºC, 7.8 mg/L, 7.58, 1.35 mg/L, 0.27 mg/L, 0.06 mg/L, and 1.7 mg/L, respectively. An increasing trend for TN/TP and a decreasing trend for pH were observed in all parts of the Liujiang. Pollution levels were generally higher in the lower Liujiang than in the upper and middle parts of the river due to the impact of urban sewage. All indicators reached level III water quality standards except TN, which suggests that the control of nitrogen emissions should be strengthened. Relatively high N/P ratios in the Liujiang contribute to a potential for phosphorus limitation of phytoplankton. The average concentration of Chlorophyll-a was 1.2 μg/L in 2014. The TLI index indicated that the eutrophication state of the Liujiang was mesotrophic, while the downstream water was polluted due to its nutrient inputs from agriculture and urban sources. The water quality of the river keeps well by comparison to other major rivers of the world, which provided the basis for urban development and river protection in Liuzhou City.
Learning from the motion principle of quadrotor, a symmetric propeller AUV, which has small size and low velocity is designed. Compared with the AUV equipped with rudders, it has better maneuverability and manipulation at low velocity. According to the Newton-Euler method, the 6 DOF kinematic model and dynamic model of the propeller AUV are established. A stability controller that consists of 3 different PID controllers is designed. It makes the depth and attitude angle as trigger conditions, and the relevant controller is chosen in different moving process. The simulation experiments simulate ideal motion state and disturbed motion state, and experiments results show that the stability controller based on combined sections method can make the best of mature technology of PID, and meet the control requirements in different stages. It has a higher respond speed and accuracy, improving the stability of the propeller AUV under the disturbance of complex ocean currents
Changing localized environmental conditions along altitude gradients could modify the responses of tree xylem structures to climate variations. To explore how trees adapt to local environment and respond to climate variants, we analysed the variation of Picea meyeri xylem tracheid features and the relationships with climate variables along an altitude gradient (1970, 2240, 2490 and 2650 m above sea level) of the Luyashan Mountains in the North China. The results mainly indicated the following: (1) tracheid number and diameter of P. meyeri showed significant differences among the four sites along the altitude gradient; (2) trees at site 2 (2240 m) and site 3 (2490 m) are similar in age, but the xylem tracheid number and diameter of these trees were significantly different, which may indicate different functional adaptation; (3) the relationships between xylem features' residual chronologies and the monthly climate data were inconsistent along altitude gradients, which indicated that the limiting factor of P. meyeri growth along the altitude gradients, shifted from drought stress at lower altitudes to low-temperature stress at higher altitudes.
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