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The morphological and histological changes which occur in the cold-induced apical meristem of broccoli (B. oleracea var. italica cv. ‘Fiesta’) during transition from the vegetative to the generative phase have been demonstrated in our previous investigations. Now, a light microscopic study on the micromorphological parameters of the tunica and corpus of the meristem was undertaken to ascertain their association with devernalisation. Changes in the apex of broccoli seedlings grown for 26 days at 24oC, succeeded by 35 days of cold treatment (16oC - vernalization) followed by 14 days of warm treatment (24oC - devernalization), were studied and described. Microscopic analysis according to the new method of slide preparation showed cytohistological zonation and the spatial distribution of cells in the central and peripheral zones of the broccoli shoot meristem more precisely than possible hitherto. Comparison between subsequent stages of meristem reorganization after devernalization revealed variation in the structure of the apex during its transition to the evocation phase of flowering. The results of measurements show that the tunica width changes irregularly, increasing from its minimum value (112.39±5.78 μm) through the medium one to the maximum values (260.32±2.50), to decrease again, while its height increased and decreased sinusoidally from its minimum value (21.52±4.30μm) to the maximum one (76.98±7.44μm) and then decreasing again to the minimum value (21.52±4.30). The stratification of the tunica as well as its width and height revealed relatively low variation within the analyzed time. These structural traits correlated with cold/warm treatments may be further utilized in the broccoli breeding programme against developmental disturbances.
Ultrastructural traits of chloroplasts in needles of Abies alba saplings growing under the canopy of Larix decidua Mill. and Picea abies Karst. were studied. An acclimation of chloroplast from the palisade parenchyma to different light conditions created by the principal species of the canopy was assessed using transmission electron microscope and light microscope. A comparison of such chloroplast patterns as: number of chloroplast per palisade parenchyma section unite, chloroplast section surface, number of thylakoids per granum, number of grana per section surface of chloroplast, thylakoids section surface to stroma section surface ratio, number of osmophilic granules, and size of starch granules allowed to determine plasticity of the ultrastructures in response to irradiance level under each canopy. There was, for example, evidence that the number of thylakoids per granum was lower, whereas chloroplast section surface inversely, was higher at greater canopy openness. The investigated patterns of chloroplasts, which reflect their biochemical adaptations, might significantly affect photosynthetic capacity of the young firs. Their variation reflected an ability of Abies alba photosynthetic apparatus to acclimate to canopy shading at the ultrastructural level.
Morphological and anatomical traits of needles of Abies alba saplings growing under the canopies of Picea abies, Fagus sylvatica, Betula pendula, Pinus sylvestris and Larix decidua were studied. An acclimation of the needles to different light conditions created by the principal species of the canopy was assessed using different microscopic methods. Length, width, needle area, stomatal density, parenchyma thickness, resin ducts diameter, central cylinder size and other traits of needles allowed to determine their structural plasticity in response to irradiance level. Leaf mass to area ratio, palisade parenchyma thickness, diameter of resin ducts increased, whereas width/thickness ratio and needle thickness/parenchyma thickness ratio decreased with increasing canopy openness. Variation in the investigated patterns of the needles reflected their structural plasticity and also an ability of silver fir saplings to acclimate to irradiance under the canopies of diverse tree species.
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