Wyniki wyszukiwania

1

Microbiological transformations on N, S and P in degraded soil subjected to one-year remediation with various wastes

100%

Joniec J.

Polish Journal of Soil Science

|

2013

|

tom 46

|

nr 1

This study was conducted on the material originating from a reclamation experiment established in the area of the former Sulphur Mine “Jeziórko”, on a soil-less formation with a particle size distribution of weakly loamy sand, strongly acidified and with poor sorptive properties. In the particular treatments of the experiment, the following were applied to the soil-less formation: flotation lime and NPK; lime and sewage sludge; sewage sludge; mineral wool (5 cm 50 cm⁻¹), lime and NPK; mineral wool (5 cm 50 cm⁻¹), lime and sewage sludge; mineral wool (500 m3 ha⁻¹), lime and NPK; mineral wool (500 m3 ha⁻¹), lime and sewage sludge. Plots prepared in this manner were then sown with a mix of grasses. The control treatment in the experiment was the ground with no amendments. The analyses of the soil material comprised assays of the numbers of bacteria and fungi degrading protein, and of their enzymatic activities i.e. arylsulphatase and alkaline phosphatase. The waste materials applied to the degraded soil had a stimulating effect on the analyzed parameters. All of the wastes applied, and the sewage sludge in particular caused an increase in the numbers of proteolytic bacteria and in the activity of alkaline phosphatase and arylsulphatase. Sewage sludge applied separately or in combination with other wastes contributed also to an increase in the number of proteolytic fungi and to an intensification of the process of nitrification. The process of ammonification was also stimulated, but only under the effect of sewage sludge applied alone or together with lime. The application of mineral wool at the dose of 5 cm 50 cm⁻¹ in combination with lime caused a slight decrease in the activity of that process.

2

Wieloletnie badania nad fitotoksycznością gleby bielicowej nawiezionej osadem ścieków komunalno-przemysłowych z wykorzystaniem biowskaźnika Lepidium sativum

63%

Joniec J., Furczak J.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2009

|

tom 535

Badania przeprowadzono na glebie bielicowej, której poziom akumulacyjny nawieziono następującymi dawkami osadu ścieków komunalno-przemysłowych: 30, 75, 150, 300 i 600 Mg∙ha-1. Po upływie 4 tygodni glebę obsadzono wierzbą wiciową Salix viminalis L. Przez okres 5 lat oraz w 9. roku pod koniec każdego okresu wegetacyjnego w glebie z poziomu Ap oraz z głębokości 20-40 cm wykonywano 2 rodzaje fitotestu z udziałem Lepidium sativum L., jako rośliny testowej. Jeden z nich wykonano bezpośrednio w próbkach gleby pobranych z ww. kombinacji. Obejmował on pomiar siły kiełkowania nasion oraz indeksu wzrostu ww. rośliny w glebie. Drugi fitotest przeprowadzono z wodnymi wyciągami glebowymi i zbadano ich wpływ na siłę kiełkowania nasion oraz przyrost długości korzenia po upływie 2, a następnie 4 dni. Uzyskane wyniki wykazały, że zastosowany jednorazowo osad ściekowy wywołał zmiany w analizowanych parametrach rośliny testowej. Zmiany te występowały w ciągu całego okresu badawczego z różnym nasileniem. W poziomie Ap proces kiełkowania nasion w glebie podlegał stymulacji w obiektach z wyższymi dawkami osadu, ale jedynie w drugim roku. W glebie z głębokości 20-40 cm wystąpiło w okresie tym hamowanie kiełkowania, ale tylko pod wpływem 75 i 150 Mg osadu na hektar. W początkowych latach doświadczenia indeks wzrostu osiągał wyższe wartości zarówno w glebie wierzchniej warstwy, jak i głębiej położonej. Natomiast w 9. roku badań stwierdzono obniżenie jego poziomu. Wyciągi glebowe z kombinacji zawierających osad ściekowy nie wywarły wpływu na proces kiełkowania nasion. Jedynie w 2. roku liczba wykiełkowanych nasion wzrosła w niewielkim stopniu w poziomie Ap. Przyrost korzenia, mierzony po upływie 2 jak i 4 dni, podlegał przez prawie cały okres badań na ogół hamowaniu w obiektach z wyższymi dawkami osadu. Oddziaływanie to silniej wystąpiło pod wpływem wyciągów glebowych z poziomu Ap.

3

Microbial populations and activity of biochemical processes related to carbon and nitrogen transformations in podzolic soil under willow culture in fifth ear from treatment with sewage sludge

63%

Furczak J., Joniec J.

Polish Journal of Environmental Studies

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2009

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tom 18

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nr 5

Our study was conducted on a podzolic soil from a field experiment, fertilized with increasing doses of fermented municipal-industrial sewage sludge: 30 Mg·ha-1 (1%), 75 Mg·ha-1 (2.5%), 150 Mg·ha-1 (5%), 300 Mg·ha-1 (10%) and 600 Mg·ha-1 (20%). Following the preparation as above, the soil was planted with basket willow (Salix viminalis L.). After five years of maintaining the plantation in Ap horizon of the soil, continued stimulation was observed in the growth of most of the microbial groups under study, i.e. oligo- and macro- trophic bacteria, filamentous fungi, cellulolytic bacteria and fungi, and a slight increase in the numbers of proteolytic bacteria. A certain intensification was also observed in the respiratory activity, rate of cellulose mineralization, nitrification, dehydrogenase and proteolytic activity. The process of ammonification, on the other hand, was subject to inhibition. In the deeper layer of the soil (20-40 cm) a positive effect of the sewage sludge was also observed, but it was notably weaker and related to only some of the aforementioned parameters (macrotrophic bacteria, filamentous fungi, cellulolytic bacteria, respiration, and rate of cellulose mineralization).

4

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Microbiological activity of soil amended with granulated fertilizer from sewage sludge

63%

Joniec J., Kwiatkowska E.

Journal of Elementology

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2014

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tom 19

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nr 1

The study was conducted on a model of a pot experiment in which grey-brown podzolic soil developed from weakly loamy sand was amended with granulated dry organic-mineral fertilizer prepared from municipal sewage sludge. Three levels of pre-sowing fertilization with the granulate were applied: I – a dose which brought nitrogen in the amount of 0.35 g kg-1 d.m. of soil, II – a 50% higher dose, and III – a 100% higher dose. Additionally, a treatment with average mineral fertilization (NPK) was run. The control treatment was non-fertilized soil. Soil prepared as above was placed in pots and sown with white mustard (cv. Borowska) in spring in the 1st and 2nd year of the experiment. During the experiment, the soil moisture was maintained at the level of ca 60% t.w.c. Microbiological and biochemical soil assays were conducted three times in the first and second years of the experiment, i.e. in May and July and 41 days from harvesting the crop and mixing of its fragmented residues with the soil. The scope of the analyses included numbers of bacteria with low and high nutrition requirements, total numbers of filamentous fungi, cellulolytic, proteolytic and lipolytic bacteria and fungi. Other analyses included determinations of the respiratory activity in soil, the rate of cellulose mineralization and the activity of dehydrogenases, lipase, protease and acid phosphatase. The study showed a stimulating effect of the sludge granulate on the above groups of bacteria and fungi, both in the 1st and in the 2nd years of the experiment. The effect generally weakened with time. The fertilization also stimulated the respiratory rate and activity of phosphatase. However, it inhibited the mineralisation of cellulose as well as the activity of dehydrogenases, lipase and protease in soil. The application of mineral fertilization in the form of NPK did not cause any changes in numbers of the analyzed bacterial groups, except a certain decreasing tendency in the second year. In both years, mineral fertilization contributed to the stimulation of the growth of most fungi. It also enhanced the respiratory activity, rate of cellulose mineralisation, and the activity of dehydrogenase and acid phosphatase, albeit only in the first year of the experiment. Finally, in the soil with mineral fertilization the activity of protease and lipase was observed to have declined.

5

Counts and activity of microorganisms participating in nitrogen transformations in soil, four years after application of sewage sludge

63%

Joniec J., Furczak J.

Journal of Elementology

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2008

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tom 13

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nr 4

545-557

The objective of the study was to determine the direction, intensity and duration of changes in abundance and activity of certain microbial groups active in nitrogen transformations in soil subjected to a 4-year fertilization treatment with municipal and industrial sewage sludge. The study was conducted on podzolic soil, whose accumulation horizon had been fertilized in 1998 with fermented sewage sludge at doses of 30 Mg⋅ha-1(1%), 75 Mg⋅ha-1(2.5%), 150 Mg⋅ha-1(5%), 300 Mg⋅ha-1(10%) and 600 Mg⋅ha-1(20%) and planted with willow (Salix viminalis L.). Four years after the application of sludge, microbiological and biochemical analyses were made in two soil horizons (0-20 cm and 20-40 cm). It was found that in the soil from the 0-20 cm depth significant stimulation of the growth of proteolytic fungi and bacteria continued, but only under the effect of the highest dose of sludge. Moreover, there was a notable stimulation of protease activity and nitrification process alongside a slight inhibition of ammonification. In the soil from the 20-40 cm layer stimulation of the growth of protein-decomposing fungi was observed as well as that of proteolytic and nitrification activity of soil, while ammonification was inhibited. However, the effect of sludge was generally less pronounced in the deeper soil layer than in the surface soil horizon.

6

Studies on stability and changes in microbiological and biochemical activity of podzolic soil under plantation of basket willow after introduction of sewage sludge

63%

Joniec J., Furczak J.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 5

Our study was conducted on a podzolic soil from a field experiment in which, 9 years earlier, municipal- industrial sewage sludge was introduced at doses of 30, 75, 150, 300, and 600 Mg·ha-1. Following the application of the sludge, the soil was planted with basket willow (Salix viminalis L.). Soil samples for the analyses were taken twice (at the beginning and at the end of the vegetation season of the 9th year) from depths of 0-20 cm and 20-40 cm. The soil material was used for determinations of the total numbers of bacteria with low and high nutrition requirements, the numbers of filamentous fungi, proteolytic bacteria and fungi, respiratory activity, rate of cellulose mineralization, rate of ammonification and nitrification, and of dehydrogenase and protease activity. The results obtained demonstrated that in the 9th year from the application of the sludge there was continued effect of its application on most of the microbiological properties (exception – proteolytic bacteria) and on all of the biochemical parameters. There was notable stimulation of the growth of cellulolytic bacteria and of the fungal groups under analysis. Increased activity was also displayed by almost all biochemical parameters, with the exception of ammonification (in both layers of the soil) and nitrification (in the deeper horizon of the soil), in which case significant inhibition was observed.

7

Liczebnosc wybranych grup drobnoustrojow w glebie bielicowej pod uprawa wierzby [Salix viminalis L.] uzyznionej osadem sciekowym w trzecim roku jego dzielania

63%

Joniec J., Furczak J.

Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura

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2007

|

tom 62

|

nr 1

105-116

8

Liczebnosc wybranych grup drobnoustrojow w glebie bielicowej pod uprawa wierzby uzyznionej osadem sciekowym w drugim roku jego dzialania

63%

Joniec J., Furczak J.

Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura

|

2007

|

tom 62

|

nr 1

94-104

9

Kształtowanie się zawartości związków fenolowych w glebie bielicowej pod uprawą wierzby wiciowej (Salix viminalis L.) na przestrzeni kilku lat od wprowadzenia do niej osadu ściekowego

63%

Joniec J., Furczak J.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2009

|

tom 535

Badania przeprowadzono na modelu doświadczenia polowego, w którym do poziomu akumulacyjnego gleby bielicowej wytworzonej z piasku słabogliniastego wprowadzono następujące dawki osadu ścieków komunalno-przemysłowych: 30, 75, 150, 300 i 600 Mg∙ha-1. Po upływie 4 tygodni glebę obsadzono wierzbą wiciową Salix viminalis L. Materiał do badań pobierano z głębokości 0-20 cm (poziom Ap) oraz 20-40 cm w 2., 3., 4., 5. i 9. roku prowadzenia plantacji. W materiale glebowym dwukrotnie każdego roku (czerwiec i październik) analizowano obecność związków fenolowych. Stwierdzono, że w poziomie Ap wystąpiły pod wpływem osadu istotne zmiany w zawartości analizowanych związków, słabnące w miarę upływu czasu, ale utrzymujące się w ciągu całego okresu badawczego. W większości obiektów w 2., 3. i 4. roku trwania doświadczenia odnotowano narastające na ogół wraz z dawką osadu podwyższone stężenie badanych związków. W 5. roku wzrost fenoli wykazano już tylko pod wpływem najwyższej dawki osadu, który w 9. roku badań przeszedł w inhibicję. Zastosowany osad ściekowy wywołał również zmiany w głębszej warstwie gleby. Jednak były one zdecydowanie słabsze i mniej ukierunkowane niż w poziomie Ap. Wzrost koncentracji połączeń fenolowych odnotowano w większości obiektów jedynie w 3. i 5. roku prowadzenia plantacji. Natomiast w 2. roku w obiektach z wyższą zawartością osadu i w roku ostatnim pod wpływem 150 Mg osadu na hektar wystąpił istotny spadek poziomu fenoli.

10

Biochemical characterization of podzolic soil under willow culture after three years from its amendment with sewage sludge

63%

Joniec J., Furczak J.

Polish Journal of Soil Science

|

2007

|

tom 40

|

nr 2

207-215

The study was conducted on a podzolic soil (originating from a field experiment), fertilized with various doses of municipal-industrial sewage sludge, i.e. 30 (1%), 75 (2.5%), 150 (5%), 300 (10%) and 600 Mg ha⁻¹ (20%), and then planted with willow (Salix viminalisL.). In the third year from setting up the experiment, in two layers of the soil (0-20 and 20-40 cm) determinations were made on the effect of the applied sludge on the respiratory activity, cellulose mineralization rate, intensification of ammonification and nitrification, and on dehydrogenases and protease activity. The results obtained showed a positive effect of the sludge on almost all of the analysed biochemical parameters, both in the surface and in the deeper layers of the soil. The effect of the sludge in the Ap horizon was more pronounced and generally increased with increasing dosage. Only the process of ammonification was subject to inhibition that was stronger in the surface horizon of the soil.

11

Studies of the effects of the level of sewage sludge crumbling on microbial and biochemical activities of soils

63%

Furczak J., Joniec J.

Polish Journal of Soil Science

|

2002

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tom 35

|

nr 1

The influence of crumbled sewage sludge on the biological properties of podzolic soil was studied under laboratory conditions during a five-month period. It was found that when compared to only slightly crumbled sludge, soil mixed with two different concentrations of crumbled sludge (1% and 10%) of a 0.5-mm particle diameter exhibited an increase in the numbers of all tested bacteria groups (total, lipolytic, cellulolytic, proteolytic). The stimulation of development of some fungi groups (cellulolytic, proteolytic) was also observed in soil with higher contents of crumbled sludge. Finally, strongly crumbled sludge stimulated most of the biochemical activities of the soil (ammonification, nitrification, dehydrogenases, lipase, protease). Such an effect was most evident in the cases of dehydrogenases and protease.

12

Changes in biochemical activity of podzolic soil under willow culture in the second year of treatment with municipal-industrial sewage sludge

63%

Furczak J., Joniec J.

International Agrophysics

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2007

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tom 21

|

nr 2

13

Preliminary study of sludge effect on soil microbial activity of a podzolic soil under willow culture

63%

Furczak J., Joniec J.

International Agrophysics

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2007

|

tom 21

|

nr 1

14

Numbers and activity of lipolytic microorganisms in podzolic soil under willow culture [Salix viminalis L.] subjected to the multi-year effect of sewage sludge

51%

Furczak J., Joniec J., Baran S.

Polish Journal of Soil Science

|

2008

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tom 41

|

nr 2

163-173

The study was conducted on podzolic soil from a field fertilised with municipal-industrial sewage sludge at the following doses – 30 (1%), 75 (2.5%), 150 (5%), 300 (10%) and 600 Mg ha⁻¹ (20%) – and then planted with basket willow (Salix viminalisL.). In the 4th, 5th and 9th years of maintaining the plantation, the following determinations were made in the soil: numbers of lipolytic bacteria and fungi, and lipase activity. It was demonstrated that in the 4th and 9th years of the study, for most of the experimental treatments, there was still continued stimulation of the growth of lipolytic bacteria and fungi in soils from the 0-20 cm layer. In that soil layer for all the years of the study there was a distinct stimulation of lipase activity. The positive effect of the sludge on the numbers and activity of the above microbial groups was also observed in soils from the 20-40 cm layer, but only for some of the experimental treatments.

15

Badania wstepne nad wplywem rolniczego zagospodarowania gleby odlogowanej na jej aktywnosc mikrobiologiczna i biochemiczna

51%

Furczak J., Podstawka-Chmielewska E., Palys E., Joniec J.

Annales Universitatis Mariae Curie-Skłodowska. Sectio E. Agricultura

|

2000

|

tom 55

153-166

16

Soil lipolytic activity under the cultivation of Salix viminalis L. enriched with sewage sludge. Part I

51%

Furczak J., Joniec J., Baran S.

Polish Journal of Soil Science

|

2002

|

tom 35

|

nr 1

Studies performed in a field indicate that podzolic soil combined with sewage sludge under Salix viminalis L. cultivation exhibited an increase in lipolytic bacteria and fungi numbers as well as a clear stimulation of lipase activity at level A. These changes increased in accordance with the increase of sludge concentration in the soil, and remained in the second and the third year of the experiment. However, a decrease of lipase activity was observed in subjects with a higher sludge concentration in the third year. Disturbances of microbial and enzymatic soil properties after the addition of sewage sludge occurred less intensively at the 20-40 cm than the 0-20 cm layer. In general, a slight stimulation of lipolytic bacteria and fungi and a stronger stimulation of lipase activity was found with higher doses of sludge. As at level A, these changes remained active for two years, showing a decline of lipase activity in the third year.

17

Aktywność enzymatyczna gleby bielicowej pod żytem ozimym uprawianym w zmianowaniu i monokulturze

51%

Furczak J., Joniec J., Derylo S.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2001

|

tom 478

18

Antiviral activity of novel oseltamivir derivatives against some influenza virus strains

45%

Kocik J., Kolodziej M., Joniec J., Kwiatek M., Bartoszcze M.

Acta Biochimica Polonica

|

2014

|

tom 61

|

nr 3

19

Research on prevention and treatment of hemorrhagic fevers

45%

Joniec J., Kolodziej M., Bartoszcze M., Kocik J., Knap J.

Annals of Agricultural and Environmental Medicine

|

2012

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tom 19

|

nr 2

Viral hemorrhagic fevers are severe zoonotic diseases caused by RNA-viruses classified into 4 families: Arenaviridae, Bunyaviridae, Filoviridae, and Flaviviridae. They are present on all continents except Antarctica, their person-to-person spread is easy, and there is a high risk of them being used as weapon by bioterrorists. So far, efforts to develop effective drugs against these viruses have failed, and typical therapy usually relies on symptomatic treatment. Search for substances that could effectively inhibit this type of infections is now a priority. The presented paper gives an overview of different approaches used in combating the viral hemorrhagic fevers. Researchers look for safe antiviral agents with appropriate properties among natural sources, such as various types of herbs plants, algae, or essential oils obtained from trees, as well as investigate the use of various synthetic substances. The aim is to broaden the pool of available antiviral drugs that could replace hitherto applied medicines such as ribavirin, which is not always sufficiently effective and may have side-effects. The scientists focus not only on combating the diseases, but also on their prevention. For this purpose, recombinant vaccines or various types of immunomodulators may serve as a useful tools. Results of the latest studies are promising and encourage further work which may eventually lead to the solution of the urgent problem of hemorrhagic fevers.

20

Identyfikacja Francisella tularensis techniką real - time PCR z wykorzystaniem sond hybrydyzujących zaprojektowanych dla fragmentów sekwencji genów fopA i tul4

39%

Bielawska-Drozd A., Niemcewicz M., Gawel J., Bartoszcze M., Graniak G., Joniec J., Kolodziej M.

Medycyna Doświadczalna i Mikrobiologia

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2010

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tom 62

|

nr 4

Badano przydatność zaprojektowanych starterów FopA F/R, Tul4 F/R i sond hybrydyzujących FopA S1/S2, Tul4 S1/S2 dla genów fopA i tul4 do wykrywania F. tularensis. W badaniach, w których użyto 50 szczepów F. tularensis uzyskano wyniki dodatnie. Swoistość reakcji wykazano badając szczepy bakterii non-Francisella tularensis. Przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 zaprojektowanych dla genów fopA dodatnie wyniki amplifikacji uzyskano ze wszystkimi badanymi szczepami F. tularensis. Identyczne wyniki otrzymano w reakcji real - time PCR z zastosowaniem starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 zaprojektowanych dla genu tul4. Swoiste produkty reakcji amplifikacji pojawiły się między 16 a 18 cyklem reakcji. Badania z użyciem starterów i sond hybrydyzujących zaprojektowanych dla genu fopA wykazały, że charakterystyczna temperatura topnienia produktów wynosiła 61°C, a dla genu tul4 60°C. Przy użyciu starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 czułość oznaczeń wynosiła 10 fg/µl, a przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 1 fg/µl.

Ograniczanie wyników

Microbiological transformations on N, S and P in degraded soil subjected to one-year remediation with various wastes

Wieloletnie badania nad fitotoksycznością gleby bielicowej nawiezionej osadem ścieków komunalno-przemysłowych z wykorzystaniem biowskaźnika Lepidium sativum

Microbial populations and activity of biochemical processes related to carbon and nitrogen transformations in podzolic soil under willow culture in fifth ear from treatment with sewage sludge

Microbiological activity of soil amended with granulated fertilizer from sewage sludge

Counts and activity of microorganisms participating in nitrogen transformations in soil, four years after application of sewage sludge

Studies on stability and changes in microbiological and biochemical activity of podzolic soil under plantation of basket willow after introduction of sewage sludge

Liczebnosc wybranych grup drobnoustrojow w glebie bielicowej pod uprawa wierzby [Salix viminalis L.] uzyznionej osadem sciekowym w trzecim roku jego dzielania

Liczebnosc wybranych grup drobnoustrojow w glebie bielicowej pod uprawa wierzby uzyznionej osadem sciekowym w drugim roku jego dzialania

Kształtowanie się zawartości związków fenolowych w glebie bielicowej pod uprawą wierzby wiciowej (Salix viminalis L.) na przestrzeni kilku lat od wprowadzenia do niej osadu ściekowego

Biochemical characterization of podzolic soil under willow culture after three years from its amendment with sewage sludge

Studies of the effects of the level of sewage sludge crumbling on microbial and biochemical activities of soils

Changes in biochemical activity of podzolic soil under willow culture in the second year of treatment with municipal-industrial sewage sludge

Preliminary study of sludge effect on soil microbial activity of a podzolic soil under willow culture

Numbers and activity of lipolytic microorganisms in podzolic soil under willow culture [Salix viminalis L.] subjected to the multi-year effect of sewage sludge

Badania wstepne nad wplywem rolniczego zagospodarowania gleby odlogowanej na jej aktywnosc mikrobiologiczna i biochemiczna

Soil lipolytic activity under the cultivation of Salix viminalis L. enriched with sewage sludge. Part I

Aktywność enzymatyczna gleby bielicowej pod żytem ozimym uprawianym w zmianowaniu i monokulturze

Antiviral activity of novel oseltamivir derivatives against some influenza virus strains

Research on prevention and treatment of hemorrhagic fevers

Identyfikacja Francisella tularensis techniką real - time PCR z wykorzystaniem sond hybrydyzujących zaprojektowanych dla fragmentów sekwencji genów fopA i tul4