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Content of pyrrolizidine alkaloids in the leaves of coltsfoot (Tussilago farfara L.) in Poland

100%

Adamczak A., Opala B., Gryszczynska A., Buchwald W.

Acta Societatis Botanicorum Poloniae

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2013

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tom 82

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nr 4

Coltsfoot (Tussilago farfara L.) is a common species, widely used in European and Chinese traditional medicine for the treatment of respiratory diseases. However, raw material from this plant contains hepatotoxic pyrrolizidine alkaloids (PAs). The aim of the study was to determine the variability of the level of PAs (senkirkine and senecionine) in leaves of coltsfoot originated from natural populations in Poland. In the phytochemical analysis, 20 samples of T. farfara were used. This plant material was obtained from the Garden of Medicinal Plants in Plewiska near Poznań and originated from different regions of Poland. Coltsfoot leaves were harvested in the middle of July of 2010 and then dried at room temperature. The alkaloid content was detected using the HPLC-DAD method. The amount of PAs in leaves of T. farfara changed in a wide range from 0.06 to 1.04 μg g−1 of dry matter (DM). The content of senkirkine and senecionine was positively correlated (r = 0.68, P = 0.001). There was no statistically significant correlation between the amount of PAs as well as leaf weight and water content in leaves of T. farfara. Our results showed that a medium-sized leaf of coltsfoot (0.33 g DM) may contain from 0.02 to 0.34 μg of PAs (on average 0.14 μg). The level of PAs was not associated with the region of Poland, but phytochemical similarity of samples was usually visible at the local scale. Coltsfoot leaves are characterized by a high variability of the content of toxic PAs, much higher than in the case of the main active compounds, especially flavonoids and mucilage. This phytochemical variability is mainly genetically determined (samples came from a garden collection), and it can be increased by environmental factors. Our investigations indicate that Polish natural populations of T. farfara may provide raw material with a low level of toxic PAs.

2

Yield and level of phenolic compounds in the inflorescence of yellow everlasting Helichrysum arenarium (L.) Moench collected from natural sites

88%

Forycka A., Adamczak A., Opala B., Gryszczynska A., Buchwald W.

Herba Polonica

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2016

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tom 62

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nr 4

Introduction: The inflorescence of Helichrysum arenarium (L.) Moench shows anti-inflammatory, antioxidant, detoxifying properties and is traditionally used in liver and biliary tract diseases. Because of its difficult and expensive cultivation, the plant raw material is mainly harvested from natural sites. Objective: The research aimed to determine the local variation in yield and content of flavonoids and phenolic acids in the yellow everlasting inflorescence against the background of the layer structure of vegetation as the rate of plant succession. Methods: The plant raw material was collected from 30 plots of 1 m2, established for three separate populations developing on sandy fallows near Zielona Góra (western Poland). For each study area, percentage cover of the moss-lichen and herb layers, the height, cover and yield of H. arenarium as well as the height and cover of other herbaceous plants were determined. Total contents of flavonoids (expressed as quercetin) and phenolic acids (calculated as caffeic acid) were measured spectrophotometrically, according to Polish Pharmacopoeia. Results: Everlastings reached a cover of up to 70% and the maximum air-dry matter yield of 46.42 g/m2. The height, coverage and yield of H. arenarium were correlated with the parameters describing the herb layer. The content of flavonoids ranged from 0.56 to 0.99%, while that of phenolic acids from 0.82 to 1.80% DM. Conclusions: Yellow everlasting is an important species of early fallows on poor sandy soils and these habitats constitute a rich natural source of herbal raw material. Inflorescences harvested from natural sites are distinguished by a high and similar content of polyphenols and usually meet the requirements of Polish Pharmacopoeia.

3

Content of alkaloids and flavonoids in celandine (Chelidonium majus L.) herb at the selected developmental phases

76%

Seidler-Lozykowska K., Kedzia B., Bocianowski J., Gryszczynska A., Lowicki Z., Opala B., Pietrowiak A.

Acta Scientiarum Polonorum. Hortorum Cultus

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2016

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tom 15

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nr 4

The content of alkaloids and flavonoids and the yield of herb were analyzed in greater celandine cultivar ‘Cynober’ during six following phases: spring rosette formation, the beginning of flowering, full bloom, green fruit, seed harvest, fall rosette formation. Yield of celandine herb was different at the investigated phases and in years of cultivation. The highest yield of herb was observed at the beginning of flowering, then a decrease was noticed, up to the phase of seed harvest, when the yield grown up. The lowest yield of celandine herb was obtained in last phase – fall rosette formation, except 2011 when the lowest yield was in spring rosette formation. The average content of alkaloids was the highest in phase of green fruit (1.097%), while in 2012, the highest content was reached in phase of fall rosette formation – 1.200%. The lowest content of alkaloids was obtained in herb of the beginning of flowering (0.608%) in both years. The mean content of flavonoids was from 0.310% (the beginning of flowering) to 0.522% (seed harvest) and was the same in both years. The stable high content of total alkaloids and flavonoids and individual alkaloids was noticed in phase of fall rosette. Our results suggest that seed maturity is the best time for celandine herb harvest regarding the herb yield and content of alkaloids and flavonoids.

4

Cold storage of Tussilago farfara L. nodal cultures

76%

Dreger M., Gorska-Paukszta M., Gryszczynska A., Opala B., Buchwald W., Krajewska-Patan A., Wielgus K.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2013

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tom 55

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nr 2

5

52%

Gryszczynska A., Opala B., Krajewska-Patan A., Lowicki Z., Buchwald W., Mielcarek S., Bogacz A., Karasiewicz M., Boron D., Czerny B., Mrozikiewicz P. M.

Herba Polonica

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2013

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tom 59

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nr 1

The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.

Ograniczanie wyników

Content of pyrrolizidine alkaloids in the leaves of coltsfoot (Tussilago farfara L.) in Poland

Yield and level of phenolic compounds in the inflorescence of yellow everlasting Helichrysum arenarium (L.) Moench collected from natural sites

Content of alkaloids and flavonoids in celandine (Chelidonium majus L.) herb at the selected developmental phases

Cold storage of Tussilago farfara L. nodal cultures

Effect of yeast elicitor on Salvia przewalskii Maxim. suspension culture

Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

Determination of lotaustralin in Rhodiola species

Content of pyrrolizidine alkaloids (senecionine and senkirkine) in Tussilago farfara L. plants cultivated in vitro

Determination of chlorogenic and gallic acids by UPLC-MS/MS

Novel method of detection of phenylpropanoids of Rhodiola roots species