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Trichinellosis is still one of the most important food-borne parasitic zoonoses and is considered as a threat to public health worldwide. The aim of this study was to use genotyping techniques to determine the prevalence of Trichinella species in wild raccoon dogs (Nyctereutes procyonoides) in western Poland. The infection rate in raccoon dogs was 0.8%. All infections were due to T. spiralis.
Giardia duodenalis is an ubiquitous flagellate that infects humans and many species of animals. This species exhibits great biotypic and genetic diversity. In the present study, we established short- and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson’s gazelle (artiodactyls) and genetically characterised the isolates by their glutamate dehydrogenase and triose phosphate isomerase gene sequences. The G. duodenalis isolates from red deer and the gazelle represented assemblages A (AIII sub-assemblage) and B. In conclusion, G. duodenalis assemblages and sub-assemblages can be associated with differences in growth rate in vitro cultures.
A new species, Stibarokris hydrobates sp. nov. is described from Hydrobates pelagicas (Linnaeus) (Procellariiformes: Hydrobatidae) from Malta. This species differs from other representatives of the genus Stibarokris Kethley, 1970 in the absence of the projection on the proximal end of the stylophore (vs. presence in all other species) and in the presence of the basal angles on tarsal claws I-IV (vs. absence). The following syringophilid species are recorded from new hosts of aquatic birds: Stibarokris phoeniconaias (Skoracki and OConnor, 2010) from Phoenicopterus chilensis Molina (Phoenicopteriformes: Phoenicopteridae) from South America, Stibarokris dastychi Głowska and Skoracki, 2011 from Phalacrocorax pygmeus Pallas (Suliformes: Phalacrocoracidae) from Greece, Procellariisyringophilus bulwerius Kethley, 1970 from Calonectris diomedea (Scopoli) (Procellariiformes: Procellariidae) from Spain and Ardenna pacifica (Gmelin) (Procellariidae) from Indonesia.
Inappropriate doses and quality of dietary nutrients cause problems in providing the protein and energy balance in a feed ration. Especially, energy value of the feed ration poses many problems to dairy cattle breeders and particularly in the perinatal period, which results in increased incidence of metabolic disorders. These disorders are today one of the most frequent causes of culling of dairy cows, as they underlie most of the disease entities. The aim of this experiment was, therefore, to verify the hypothesis that oleic acid (OA) can be used as a biomarker for early diagnosis of elevated blood levels of non-esterified fatty acids (NEFAs) and β-hydroxybutyric acid (BHBA) in the early stages of lactation in high-yielding Polish Holstein (PHF) cows. The highest blood levels of NEFAs and BHBA of 1.573 and 1.116 mmolL⁻¹, respectively, was associated with the highest content of OA in milk fat. High concentrations of both NEFAs and BHBA, indicating explicitly the occurrence of the metabolic disease in cows, occur when the content of OA in milk exceeds 24g 100g⁻¹ of fat. Oleic acid may be used as a biomarker for the early diagnosis of elevated blood levels of NEFAs and BHBA in the early stages of lactation in high-yielding PHF cows.
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