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1

12-O-tetradecanoylphorbol-1,3-acetate-induced degradation of protein kinase B via ubiquitin-proteasomal pathway depends on its Ser473 phosphorylation in gastric cancer cells

100%
Zhang B., Xia C.
Folia Histochemica et Cytobiologica
|
2013
|
tom 51
|
nr 1
2

The expression of protein kinase B in gastric cancer cell apoptosis induced 12-O-tetradecanoylphorbol-1,3-acetate

100%
Zhang B., Xia C.
Cellular and Molecular Biology Letters
|
2009
|
tom 14
|
nr 3
466-480
Protein kinase B (PKB/Akt) is a serine-threonine kinase functioning downstream of phosphatidylinositol 3-kinase (PI-3 kinase) in response to mitogen or growth factor stimulation. In several cell types, it plays an important anti-apoptotic role. TPA is a potent regulator of the growth of many different cell types. Here, we detected that TPA could induce cell apoptosis in the gastric cancer cell line, BGC-823. We also found that TPA inhibited the expression of PKB/Akt in a TPA concentration- and time-dependent manner. Furthermore, TPA inhibited the phosphorylation of PKB at Ser473, but did not affect the phosphorylation of Thr308. It only attenuated the expression of PKB/Akt and the phosphorylation of Ser473 in the cell nucleus, whereas it did not change the PKB/Akt distribution in BGC-823 cells. These results suggest that PKB/Akt inhibition by TPA may be the important factor in the mechanism of effect of TPA on gastric cell lines.
3

12-O-tetradecanoylphorbol-1,3-acetate induces the negative regulation of protein kinase B by protein kinase Calpha during gastric cancer cell apoptosis

100%
Zhang B., Xia C.
Cellular and Molecular Biology Letters
|
2010
|
tom 15
|
nr 3
377-394
The PKB signaling pathway is essential for cell survival and the inhibition of apoptosis, but its functional mechanisms have not been fully explored. Previously, we reported that TPA effectively inhibited PKB activity and caused PKB degradation, which was correlated with the repression of PKB phosphorylation at Ser473. In this study, we focus on how PKB is regulated by TPA in gastric cancer cells. One of the TPA targets, PKCα, was found to mediate the inhibition of PKB phosphorylation and degredation caused by TPA. Furthermore, TPA induced the import of PKCα into the nucleus, where PKCα exerted an inhibitory effect on PKB expression and phosphorylation. As a result, cancer cell proliferation was arrested. Our study characterizes a novel function of PKCα in mediating the negative regulation of PKB by TPA, and suggests a potential application in the clinical treatment of gastric cancer.
4
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Plasma metabolic differences in cows affected by inactive ovaries or normal ovarian function post partum

81%
Zhao C., Hu P., Bai Y. L., Xia C.
Polish Journal of Veterinary Sciences
|
2020
|
tom 23
|
nr 1
5

Increased apoptosis in human knee osteoarthritis cartilage related to the expression of protein kinase B and protein kinase Calpha in chondrocytes

81%
Chen Q., Zhang B., Yi T., Xia C.
Folia Histochemica et Cytobiologica
|
2012
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tom 50
|
nr 1
6

Expression pattern of transcription factor SOX2 in reprogrammed oligodendrocyte precursor cells and microglias: Implications for glial neurogenesis

81%
Zhang Y., Qi Y., Liu Z., Xia C.
Acta Neurobiologiae Experimentalis
|
2014
|
tom 74
|
nr 1
Oligodendrocyte Precursor Cells (OPCs) can revert to multipotential Neural Stem-Like Cells (NSLCs) which can self-renew and give rise to neurons, astrocytes and oligodendrocytes when exposed to certain extracellular signals. This is a significant progress to understand developmental neurobiology, in particularly the possibility of converting glia to stem cells for the treatment of neurological disorders. Similarly, recent findings revealed that brain-resident microglias (MGs) can be converted to multipotential state through de-differentiation. In this study, we investigated the role of SRY (sex-determining region)-box 2 (SOX2), a high-mobility group DNA binding domain transcription factor, in the reprogramming of OPCs and MGs and molecular pathways involved in these process. Immunocytochemical analyses demonstrated that expression of SOX2 was upregulated in the reprogrammed MGs and OPCs as well as other neural stem cell markers such as CD15 and nestin. Western blot and double immunostaining analyses further confirmed that activation of bone morphogenetic proteins (BMPs) signaling partnering with SOX2 might be one of the molecular pathways involved in lineage reprogramming of OPCs which is also true in the reversion of MGs. Taken together, these results indicated that lineage reprogramming of OPCs and MGs are both controlled by the same signaling pathway and glia can be reprogrammed in culture by inducing expression of neurogenic transcription factors to transgress their lineage restriction and can stably acquire a neuronal identity. Our results suggested innovative perspectives for cell therapy with glia cells.
7

Effects of ketosis in dairy cows on blood biochemical parameters, milk yield and composition, and digestive capacity

71%
Yang W., Zhang B., Xu C., Zhang H., Xia C.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 4
8

Effect of negative energy balance on plasma metabolites, minerals, hormones, cytokines and ovarian follicular growth rate in Holstein dairy cows

61%
Song Y., Wang Z., Zhao C., Bai Y., Xia C., Xu C.
Journal of Veterinary Research
|
2021
|
tom 65
|
nr 3
9

A note on the distribution of insulin receptor mRNA in tissues of neonatal Holstein calves

61%
Liu G. W., Zhang Z. G., Zhu X. L., Wang Z., Xia C., Xu C.
Journal of Animal and Feed Sciences
|
2009
|
tom 18
|
nr 4
10

2-DE-MS based proteomic investigation of dairy cows with footrot

61%
Zheng J., Shu S., Xia C., Xu C., Zhang H., Xu C.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 1
11

Plasma metabolic characterisation of dairy cows with inactive ovaries and oestrus during the peak of lactation

61%
Song Y. X., Hu P., Bai Y. L., Zhao C., Xia C., Xu C.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 3
12

Stable isotope characteristics for precipitation events and their responses to moisture and environmental changes during the summer monsoon period in Southwestern China

61%
Xia C., Liu G., Chen K., Hu Y., Zhou J., Liu Y., Mei Y.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 3
13

Spatial spillover effect and sources of city-level haze pollution in China: a case study of Guangdong Provinces

61%
Liao S., Wang D., Liang Z., Xia C., Guo H., Zhao W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 5
14

17beta-Estradiol promotes cell proliferation in rat osteoarthritis model chondrocytes via PI3K/Akt pathway

61%
Huang J., Xia C., Zheng X., Yi T., Wang X., Song G., Zhang B.
Cellular and Molecular Biology Letters
|
2011
|
tom 16
|
nr 4
Osteoarthritis (OA) is the most common cause of musculoskeletal pain and disability. The importance of chondrocytes in the pathogenesis of OA is unequivocal. 17β-estradiol (E2) has a potential protective effect against OA. However, the mechanism of E2 in OA chondrocytes remains unclear. In this study, we investigated the regulative effect of E2 on cell growth and the relationship between E2 and the PI3K/Akt pathway in rat OA model chondrocytes (pretreated with interleukin-1β). We found that E2 induced chondrocyte proliferation, and increased the expression level of Akt simultaneously, especially the expression level of P-Akt. Furthermore, the inhibition of P-Akt could block chondrocyte proliferation induced by E2. These results suggest that PI3K/Akt activation induced by E2 may be an important factor in the mechanism of E2 in cell proliferation in rat OA model chondrocytes, and help further understanding the role of E2 in OA progression.
15

Sequence analysis of human cytomegalovirus US28 gene in low-passage clinical isolates from children and AIDS patients

61%
He R., Xia C., Ruan Q., Ma Y.-P., Ji Y.-H., Guo J.-J.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 2
Human cytomegalovirus (HCMV) is often a dangerous opportunistic pathogen that causes significant morbidity and mortality in newborn children and immunocompromised patients. The different symptoms and tissue tropisms of HCMV infection may result from genetic polymorphism. This study investigated the sequence variability of the HCMV US28 ORF, which shows sequence homology to the G protein-coupled receptor. HCMV isolated from suspected pediatric cases and isolates from AIDS patients were compared in order to examine the possible associations between polymorphisms and pathogenesis. Seventy children with suspected congenital HCMV infection, who suffered from jaundice (47), megacolon (10), and microcephaly (13), and 17 AIDS patients, were studied. Mutation was prevalent among the sequences of US28, with a focus on the two ends of US28. The important functional groups of US28 are highly conserved. An unrooted tree showed that all sequences from suspected congenitally infected infants and AIDS patients were divided into three groups. Comparison showed that most of the sequences (12/17) from pediatric patients were included in the first group (G1), whereas most of the sequences (11/17) from AIDS patients were included in the third group (G3). The specific high mutation sites in US28 from children were located at the C terminus of the protein, whereas those from AIDS patients were located at the N terminus. We demonstrated the existence of polymorphisms among the US28 genes of clinical isolates of HCMV from infants with suspected congenital infection. Comparison of US28 sequences from AIDS patients with those from children showed that both sequences have their own specific high mutation points.
16

Identification of novel pathways in pathogenesis of ketosis in dairy cows via iTRAQ/MS

51%
Shu S., Xu C., Xia C., Xiao X., Wang G., Fan Z., Cao Y., Wang Y., Zhang H.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 3
17

Metastasis of human gastric adenocarcinoma partly depends on phosphoinositide-specific phospholipase gamma1 expression

51%
Zhuang L., Zhang B., Zeng G., Dai L., Qian H., Hu T., Song G., Zhang B., Xia C.
Folia Histochemica et Cytobiologica
|
2014
|
tom 52
|
nr 3
18
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Identification of three species commonly known as“daqingye” by internal leaf anatomy and high-performance liquid chromatography analyses

51%
Zhang Y., Zhu Y., Chen J., Xia C., Deng J., Li H., Li Y., Li J., Liu P.
Acta Societatis Botanicorum Poloniae
|
2018
|
tom 87
|
nr 1
The macroscopic and microscopic morphologies and indigo and indirubin concen­tration of the traditional Chinese medicine herbs Isatis indigotica Fort., Polygonum tinctorium Ait., and Baphicacanthus cusia (Nees) Bremek, all commonly known as “daqingye”, were determined and compared. The morphological analyses indicated that I. indigotica has leaves with winged petioles and no glandular hairs or crystals, P. tinctorium has leaves with membranous ocrea and clusters of calcium oxalate, and B. cusia has palisade cells in the mesophyll running over the main vein and single cells containing calcium carbonate crystals. Indigo and indirubin are chemical constituents that have been previously isolated from daqingye and were selected in this study as identification markers for high-performance liquid chromatography analysis due to their pharmacological activities. The chromatographic results showed that indigo and indirubin concentration varied significantly among the three species: high concentration of both indigo and indirubin were observed in I. indigotica, the highest concentration among the three daqingye plants was found in P. tinctorium but with low levels of indirubin, and the concentration of indigo and indirubin was quite low in B. cusia. In summary, three different species commonly known as daqingye were accurately distinguished by morphological observation, internal leaf anatomy analysis, and chromatographic analysis.
19

Influence of oleic acid in different solvent media on BRL 3A cell growth and viability

51%
Liu R., Yang W., Xia C., Chen Y., Gao S., Dong Z., Huang B., Li R., He P., Xu C.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr 3
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