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1

Importance of the malate-asparate shuttle for the reoxidation of glycolytically produced nadh and for cell aggregation in porcine blood platelets

100%
Tomasiak M.
Acta Biochimica Polonica
|
1987
|
tom 34
|
nr 3
2

Mechanizmy transdukcji sygnału w procesie aktywacji płytek krwi

100%
Tomasiak M.
Postępy Biologii Komórki
|
1990
|
tom 17
|
nr 3
3

Effect of rise in intracellular Na and-or cytosolic alkalinization on the aggregation of porcine blood platelets

100%
Tomasiak M.
Applied Biology Communications. Lublin Scientific Center
|
1992
|
tom 02
|
nr 6-7
4

Protein kinase c-mediated activation of Na - H exchanger in porcine blood platelets

100%
Tomasiak M.
Applied Biology Communications. Lublin Scientific Center
|
1992
|
tom 02
|
nr 3
5

Relationship between intracellular pH and aggregation in porcine blood platelets

100%
Tomasiak M.
Applied Biology Communications. Lublin Scientific Center
|
1992
|
tom 02
|
nr 2
6

Evidence for existing the Na - H exchanger in porcine blood platelets

100%
Tomasiak M.
Applied Biology Communications. Lublin Scientific Center
|
1992
|
tom 02
|
nr 1
7

Inhibitory effect of quercetin on glycolysis in blood platelets

100%
Tomasiak M.
Applied Biology Communications. Lublin Scientific Center
|
1992
|
tom 02
|
nr 1
8

The involvement of Na+/H+ exchanger in the desmopressin-induced platelet procoagulant response

71%
Tomasiak M., Stelmach H., Bodzenta A., Tomasiak M.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
9

In vitro efeect of integrilin, glycoprotein IIb/IIIa antagonist, on various responses of porcine blood platelets

63%
Ciborowski M., Tomasiak M.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
10

On the mechanism of glycolysis stimulation by mitochondria from Guerin epithelioma

63%
Tomasiak M., Rzeczycki W.
Acta Biochimica Polonica
|
1988
|
tom 35
|
nr 3
11

Laktadheryna male bialko o wielkich mozliwosciach

63%
Misztal T., Tomasiak M.
Postępy Biologii Komórki
|
2011
|
tom 38
|
nr 1
143-158
12

Cyslosporine A and platelet procoagulant activity

51%
Rusak T., Tomasiak M., Stelmach H., Gacko M.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
13

The role of the Na+/K+ -ATPase the generation of procoagulant activity in blood platelets

51%
Stelmach H., Tomasiak M., Rusak T., Ciborowski M.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
14

Microenvironmental factors modulating peroxynitrite action on porcine blood platelets

51%
Rusak T., Stelmach H., Tomasiak M.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr Supplement 1
15

Vasopressin- induced platelet procoagulant response

51%
Tomasiak M., Stelmach H., Rusak T., Ciborowski M.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
16

Peroxynitrite can affect platelet responses by inhibiting energy production

51%
Rusak T., Tomasiak M., Ciborowski M.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr 4
17

Jakość mleka towarowego dostarczanego do punktu skupu w zależności od skali jego produkcji w gospodarstwie

51%
Litwinczuk A., Gnyp J., Tomasiak M., Matuszczak W.
Zeszyty Naukowe. Przegląd Hodowlany
|
1999
|
tom 44
18

The role of Naplus-Hplus exchanger in serotonin secretion from porcine blood platelets

51%
Tomasiak M., Ciborowski M., Stelmach H.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 4
This study was undertaken to evaluate whether a link exists between the activation of protein kinase C (PKC), operation of Na+/H+ exchanger (NHE), cell swelling and serotonin (5-HT) secretion in porcine platelets. Activation of platelets by thrombin or phorbol 12-myristate 13-acetate (PMA), a PKC activator, initiated a rapid rise in the activity of Na+/H+ exchanger and secretion of 5-HT. Both thrombin- and PMA-evoked activation of Na+/H+ exchanger was less pronounced in the presence of ethyl-isopropyl-amiloride (EIPA), an NHE inhibitor, and by GF 109203X, a PKC inhibitor. Monensin (simulating the action of NHE) caused a dose-dependent release of 5-HT that was not abolished by GF 109203X or EGTA. Lack of Na+ in the suspending medium reduced thrombin-, PMA-, and monensin-evoked 5-HT secretion. GF 109203X nearly completely inhibited 5-HT release induced by PMA-, partly that induced by thrombin, and had no effect on 5-HT release induced by monensin. EIPA partly inhibited 5-HT release induced by thrombin and nearly totally that evoked by PMA. Electronic cell sizing measurements showed an increase in mean platelet volume upon treatment of cells with monensin, PMA or thrombin. The PMA- and thrombin-evoked rise in mean platelet volume was strongly reduced in the presence of EIPA. As judged by optical swelling assay monensin and PMA produced a rapid rise in platelet volume. The swelling elicited by PMA was inhibited by EIPA and its kinetics was similar to that observed in the presence of monensin. Hypoosmotically evoked platelet swelling did not affect platelet aggregation but significantly potentiated thrombin-evoked release of 5-HT and ATP. Taken together, these results show that in porcine platelets PKC may promote 5-HT secretion through the activation of NHE. It is hypothesized that enhanced Na+/H+ antiport may result in a rise in cell membrane tension (due to cell swelling) which in turn facilitates fusion of secretory granules with the plasma membrane leading to 5-HT secretion.
19

Nitric oxide and platelet energy metabolism

51%
Tomasiak M., Stelmach H., Rusak T., Wysocka J.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 3
This study was undertaken to determine whether nitric oxide (NO) can affect platelet responses through the inhibition of energy production. It was found that NO donors: S-nitroso-.N-acetylpenicyllamine, SNAP, (5-50 uM) and sodium nitro- prusside, SNP, (5-100 uM) inhibited collagen- and ADP-induced aggregation of por­cine platelets. The corresponding IC50 values for SNAP and SNP varied from 5 to 30 uM and from 9 to 75 uM, respectively. Collagen- and thrombin-induced platelet secre­tion was inhibited by SNAP (IC50 = 50 uM) and by SNP (IC50 = 100 uM). SNAP (20-100 uM), SNP (10-200 uM) and collagen (20 ug/ml) stimulated glycolysis in in­tact platelets. The degree of glycolysis stimulation exerted by NO donors was similar to that produced by respiratory chain inhibitors (cyanide and antimycin A) or uncouplers (2,4-dinitrophenol). Neither the NO donors nor the respiratory chain blockers affected glycolysis in platelet homogenate. SNAP (20-100 uM) and SNP (50-200 uM) inhibited oxygen consumption by platelets. The effect of SNP and SNAP on glycolysis and respiration was not reduced by 1H-[1,2,4]oxadiazo- lo-[4,3-a]quinoxalin-1-one, a selective inhibitor of NO-stimulated guanylate cyclase. SNAP (5-100 ,aM) and SNP (10-300 uM) inhibited the activity of platelet cytochrome oxidase and had no effect on NADH:ubiquinone oxidoreductase and succinate dehydrogenase. Blocking of the mitochondrial energy production by antimycin A slightly affected collagen-evoked aggregation and strongly inhibited platelet secre­tion. The results indicate that: 1) in porcine platelets NO is able to diminish mito- chondrial energy production through the inhibition of cytochrome oxidase, 2) the in­hibitory effect of NO on platelet secretion (but not aggregation) can be attributed to the reduction of mitochondrial energy production.
20

Involvement of Naplus-Hplus exchanger in desmopressin-induced platelet procoagulant response

51%
Tomasiak M. M., Stelmach H., Bodzenta-Lukaszyk A., Tomasiak M.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 3
Desmopressin (DDAVP) action on platelets is associated with the development of procoagulant response but the underlying mechanism of this phenomenon is not known. We investigated whether this effect of DDAVP might be due to activation of plasma membrane Na+ /H+ exchanger. The DDAVP-induced platelet procoagulant re­sponse, measured as phospholipid-dependent thrombin generation, was dose de­pendent and significantly weaker than that produced by collagen or monensin (mim­ics Na+ /H+ antiport). Both the DDAVP- and collagen-produced procoagulant re­sponses were less pronounced in the presence of EIPA, an Na+/H+ exchanger inhibi­tor. Flow cytometry studies revealed that in vitro treatment of platelets with DDAVP or collagen was associated with the appearance of both degranulated (and frag­mented) and swollen cells. The DDAVP-evoked rise in size and granularity heteroge­neity was similar to that produced by collagen or monensin and was not observed in the presence of EIPA. Using flow cytometry and annexin V-FITC as a probe for phosphatidylserine (PS) we demonstrated increased and uniform binding of this marker to all subsets of DDAVP-treated platelet population. The DDAVP-evoked PS expression was dose dependent, strongly reduced by EIPA and weaker than that caused by monensin or collagen. As judged by optical swelling assay, DDAVP in a dose dependent manner produced a rise in platelet volume. The swelling was inhib­ited by EIPA and its kinetics was similar to that observed in the presence of monensin. Electronic cell-sizing measurements showed an increase in mean platelet volume and a decrease in platelet count and platelet crit upon treatment with DDAVP. DDAVP elicited a slow (much slower than collagen) alkalinization of platelet cytosol. Altogether the data indicate an involvement of Na+/H+ exchanger in the generation of procoagulant activity in DDAVP-treated platelets.
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