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1

Power of teachers’ words - the influence on pupils’ grades and behaviour

100%
Podobinska M.
World Scientific News
|
2017
|
tom 77
|
nr 1
2

Differentiation of neural stem cells derived from human cord blood (HUCB-NSC) under low oxygen conditions

58%
Podobinska M., Szablowska-Gadomska I., Winiarska H., Buzanska L.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
In the human body, stem cells are located in niches, which are extremely complex microenvironments (with specified oxygen conditions and cellular together with extracellular matrix components arranged as a 3D structure). The influence of signals from niches seems to play an important role in maintenance of stem cells pluripotency and in their differentiation. We have been investigating the influence of the different niche components on the proliferation and differentiation of neural stem cells into specific cell types as well as the molecular mechanisms underlying this cell responses. In this study we are investigating the influence of low oxygen tension conditions on proliferation and differentiation of Human Umbilical Cord Blood of Neural Stem Cell (HUCB-NSC). Human Neural Stem Cells (NSC) in their physiological niches are exposed to 2–8% oxygen level. For that purpose, HUCB-NSC, were cultivated in two oxygen tension conditions: 21% and 5% with or without the presence of differentiation factor dBcAMP (N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt). We compared the expression of the markers characteristic for proliferation (Ki67) as well as neuronal and astroglial lineage commitment (MAP2, GFAP, β-tubulin, NF200). The presence of tested markers was revealed on the protein (immunocytochemistry) and gene expression level (Real-Time PCR). Our data show, that the low oxygen tension promote HUCB-NSC differentation into neuronal lineage. We also observed that low concentration of oxygen increases cell proliferation.Sponsored by grant from Polish Ministry of Scientific Research and Higher Education No 5978/B/PO1/2010/38
3

Two different classes of 22-kHz ultrasonic vocalizations during sexual interactions, correlation with rat behavioral state

51%
Bialy M., Podobinska M., Barski J. J., Bogacki-Rychlik W., Sulkowska-Sajdel E.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Low frequency ultrasonic vocalizations, commonly referred as 22‑kHz, occur in different situations including both aversive as well as positive emotional states in rats. Our previous research suggests that following ejaculation, long, lasting low frequency vocalizations reflect a positive emotional state – relaxation after high arousal state. It has been found that there is another type of 22-kHz vocalizations, which occurs during sexual interactions – at the time of firstseparated‑noncontact test. AIM(S): The aim of our study was to investigate the spectral analysis of low frequency ultrasonic vocalizations when male is prevented from a direct contact with estrous female and compare such calls to postejaculatory vocalizations. METHOD(S): The subjects were Long–Evans males (N=9) and female (N=9) rats 4.5 months old at the start of the experiment. The clear Plexiglas test chamber (50×25×30 cm) was used for copulatory and barrier noncontact (NC) tests. For NC tests, the chamber was bisected. Behaviors were recorded using Noldus Ethovision system simultaneously, on the same computer, with ultrasounds recording using Metris Sonotract system and analyzed manually. RESULTS: We found that about half of males prevented from a direct contact with an estrous female vocalized at frequency below 30-kHz. These vocalizations usually initiated with short signal at higher 45-kHz frequency and then transited to lower, below 30-kHz frequency. Usually these vocalizations coexist with sniffing of a hole or exploration activities – rearing or sniffing cage. The high activity state observed in separated males during low frequency vocalization is in contrast to male immobility observed during postejaculatory period when male emit extremely flat 22-kHz ultrasonic vocalizations. CONCLUSIONS: A pattern of low frequency vocalizations in rats during frustration differs significantly from that observed during relaxation state after ejaculation and correlates to the different type of behavioral activity. FINANCIAL SUPPORT: This work was supported by the Medical University of Warsaw (grant 1MA/N/170 and mini Grant for Wiktor Bogacki-Rychlik.
4

Distinct classes of low frequency ultrasonic vocalizations in rats during sexual interactions relate to different emotional states

51%
Bialy M., Podobinska M., Barski J., Bogacki-Rychlik W., Sajdel-Sulkowska E. M.
Acta Neurobiologiae Experimentalis
|
2019
|
tom 79
|
nr 1
This study examined low-frequency ultrasonic vocalizations (lUSVs) in rats during two types of sexual interactions: postejaculatory interval (PEI) and barrier – noncontact (NC) test. We report distinct classes of IUSVs that can be assigned to different emotional states; relaxation vs. frustration. Totally flat, 22-kHz calls (Class A), were observed during the relaxation state following ejaculation; characterized by immobilization or grooming during the PEI. On the other hand, twothree component IUSVs (Class B) that start at a higher frequency (45-kHz: flat, upward or short signal) and then shift to 35-23kHz (mostly to 2823-kHz), correspond as we assume, to arousal and frustration – active states associated with sniffing a hole or exploration during the NC test. We suggest that momentary, abrupt decreases of arousal during the frustration state correspond to Class B IUSVs. The detailed spectral analysis of the high-frequency component of twocomponent IUSVs is crucial for establishing the relationship between such IUSVs and the corresponding behavior and emotional states. Our studies indicate that while the twocomponent Class B 22-kHz IUSVs may relate to the frustration state, a single component, flat, Class A IUSV relates to the relaxation state. The results of these studies support a notion that rats emit distinct vocalization patterns, reflecting their emotional states.
5

Reprogramming of somatic cells: possible methods to derive safe, clinical-grade human induced pluripotent stem cells

51%
Augustyniak J., Zychowicz M., Podobinska M., Barta T., Buzanska L.
Acta Neurobiologiae Experimentalis
|
2014
|
tom 74
|
nr 4
Derivation of pluripotent stem cells from adult somatic tissues by reprogramming technology has opened new therapeutic possibilities. Current most efficient procedures for derivation of induced pluripotent stem (iPS) cells are based on the viral vectors, which represent the danger of insertional mutagenesis during incorporation of introduced genes into the host genome. To circumvent this problem, the new, safe, non-integrative and non-viral strategies of reprogramming have been developed. In this review we discuss novel DNA-free and viral-free methods of reprogramming to iPS cells including protein transduction, mRNA and microRNA delivery.
6

The modulation of HIFs, pluripotency and differentiation genes expression by oxygen conditions in neural stem cells derived from human cord blood

44%
Szablowska-Gadomska I., Drela K., Podobinska M., Ziemka-Nalecz M., Domanska-Janik K., Buzanska L.
Acta Neurobiologiae Experimentalis
|
2013
|
tom 73
|
nr 1
The oxygen tension is an important factor modulating cell fate and developmental decisions. There are evidences that HIFs (Hypoxia Inducible Factors) family is implicated in the regulation of pluripotency and differentiation genes. The goal of this study is to compare the influence of close to physiological oxygen conditions (5%) to atmospheric oxygen tension on differentiation process and pluripotent activity in HUCB-NSC. The expression of Hypoxia Inducible Factors, stemness and neural differentiation markers in NSC, cultured under 5% and 21% oxygen were checked on the transcriptional and translational level. We were looking at the interaction between HIFs (HIF1 alpha, HIF 2 alpha) and activity of neural differentiations genes (MAP2, GFAP, β-tubulin) as well as expression of pluripotency genes (Oct4, Sox2, Rex1 and Nanog). In order to demonstrate the impact of increased HIF1α and/ or HIF2α level on cell differentiation we used DMOG (Sigma) which is of prolyl-4-hydroksylase inhibitor to increase HIF alpha levels. Our data show, that low oxygen conditions promote proliferation of HUCBNSC at early stage of development and can activate Oct4 and Nanog genes in HUCB-NSC. The time of cultivation of the cells in low oxygen conditions and the developmental stage of the cells are the important factors for the induction of the expression of “pluripotency” genes.Hypoxia Inducible Factors HIF 1α and HIF 2α, but not HIF3α are expressed in HUCB-NSC at all stages of development. During neuronal differentiation of HUCB-NSC by using dBcAMP, 5% oxygen level act synergistically, promoting further differentiation (enhanced MAP2 expression). Application of prolyl hydroxylase inhibitor – DMOG resulted in increased expression of HIF1α but not HIF2α and increased the expression of MAP2 (only in 21% oxygen conditions) referring to variants without DMOG. Sponsored by grant from Polish Ministry of Scientific Research and Higher Education No N N302 597838 and by NSC grant No 2011/01/B/NZ3/05401
7

Borelioza - choroba zawodowa rolników

43%
Podobinska M.
Poradnik Gospodarski
|
2018
|
nr 10
Borelioza - choroba zawodowa rolników
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