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As an environmental factor, light influences the physiological functions and secondary metabolism of plants. However, the role of light in cotton fiber development and pigment biosynthesis has not yet been thoroughly explored. In this study, ovules of green cotton were cultured in vitro under dark and light conditions, and fiber and ovule growth parameters as well as fiber carbohydrate and cellulose contents and the expression of genes related to fiber development were investigated to elucidate the effect of light on fiber development. In addition, to investigate the effect of light on fiber pigment biosynthesis, the fiber flavonoid content and related gene expression were determined. The results demonstrated that the fiber length and the expression levels of fiber elongation genes under light culture were significantly lower than under dark culture, however, the ovule and fiber weight were significantly higher than under dark culture. The fiber developed under light culture had higher carbohydrate concentrations and carbohydrate transformation rate than under dark culture. Additionally, light culture exhibited higher cellulose contents and expression levels of cellulose biosynthesis genes compared with dark culture. In contrast, the pattern of the effect of light on flavonoid biosynthesis differed from that for cellulose biosynthesis. At 10 DAC (days after culture) and 20 DAC, the flavonoid contents and the expression levels of genes related to flavonoid biosynthesis were lower than under dark culture. However, the flavonoid contents and gene expression levels observed at 30 DAC and 40 DAC were higher in the light culture than in the dark culture. These results suggested that light hindered fiber elongation, but promoted carbohydrate accumulation and carbohydrate transformation, which resulted in fiber weight gain and increased cellulose accumulation in fibers. In addition, light inhibited flavonoid biosynthesis at early stage of fiber development, but promoted it at later stages. These findings provide the basis for intensive study of fiber development and flavonoids biosynthesis in green cotton.
The 9-cis-epoxycarotenoid dioxygenase (NCED) is thought to be the rate-limiting enzyme in the abscisic acid (ABA) biosynthetic pathway. In this study, transient expression of AhNCED1 and ABA distribution were detected in the vascular cambium of a drought-tolerant peanut cultivar (Yueyou 7) under a water stress treatment. It caused increases in ABA content in this region. The synthesis of ABA and AhNCED1 in the leaves of Yueyou 7 took place more quickly than in the control cultivar (Shanyou 523). Furthermore, AhNCED1 mRNA and proteins were induced in Yueyou 7 than in Shanyou 523, coinciding with greater ABA accumulation. During the seedling, blooming, and fruiting stages, AhNCED1 protein expression was higher in Yueyou 7 than in Shanyou 523, and it was induced more quickly when the plants were under water stress. These data suggest that the drought-tolerant cultivar can synthesize and distribute ABA more rapidly than does the control cultivar because of a high level of AhNCED1 expression, which then modulates physiological responses under water stress conditions.
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Ethnobotany of Acorus in China

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In China, species in the genus Acorus are used for many different purposes, viz., in traditional medicine, for rituals, and as ornamental plants. Acorus calamus (sweet flag) has been a symbolic plant in Chinese culture for many centuries and is used as a ceremonial object in festivals throughout the country. For other Acorus species in China, ethnobotanical records are few. During field investigations from 2006 to 2017, we interviewed 573 individuals representing ethnic groups such as the Miao, Yao, Buyi, Shui, Tujia, Dong, She, Maonan, Zhuang, Yi, and Han people. Various ethnobotanical approaches were adopted in the field surveys, including key informant interviews, semistructured interviews, participatory observation, direct observation, etc. The results have revealed a rich treasure trove of traditional knowledge on Acorus in China. Two species (A. macrospadiceus and A. gramineus) were used by ethnic groups as spices to add flavor to meat. Through the study found that all the species of Acorus had medicinal value, especially A. tatarinowii, deafness, blindness, and digestive disorders were among the conditions commonly treated using these plants. Traditional knowledge of the cultural, ethnomedicinal, and food values of Acorus, based on our recent literature surveys and field investigations in China is presented here. A better understanding of Acorus is vital for conserving the plants and the traditional knowledge associated with them.
Variation laws of growth conditions and root cortical aerenchyma (RCA) of 15 common maize hybrids (Zea mays L.) in Yunnan Province under simulated rare extreme drought and rehydration conditions were tested in this paper. The relationship between functions of root tissues in extreme drought process and resilience after rehydration was discussed. Results demonstrated: 1) in middle drought period, RCA area is closely related with drought resistance of varieties and 2) Varieties with large or small RCA area under drought stress have poor resilience after rehydration, while varieties with middle RCA area present strong resilience. We concluded that RCA area formed under drought stress will significantly influence recovery of root functions during rehydration. Large RCA area brings corn strong drought resistance, but it goes against recovery of root functions after rehydration. Small RCA area causes poor drought resistance of corn plants, which will induce serious damage to plants and make it difficult to recover root functions after rehydration. Moderate RCA area helps corn variety to develop certain drought resistance and recover functions of root tissues through further formation of RCA area by parenchymal cells in root cortical tissues. RCA area formed in corn roots can affect drought resistance significantly, and increased RCA area in root system after rehydration can enhance resilience of corn plants.
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