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1

Determination of staphylococcal enterotoxins in milk and milk products by three methods

100%
Burdova O., Dudrikova E., Gasincova E., Pleva J.
Archivum Veterinarium Polonicum
|
1994
|
tom 34
|
nr 1-2
69-74
2
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Levels of residual nitrates and nitrites in milk in 1996

100%
Baranova M., Burdova O., Mal'a P., Zezula I.
Bulletin of the Veterinary Institute in Puławy
|
1998
|
tom 42
|
nr 2
Levels of nitrates and nitrites were observed in pooled samples of milk from selected suppliers of the Košice dairy, and in dairy treated milk during certain months of the year. The average value of nitrates in examined (432) pooled samples of milk ranged from 1.84 to 4.58 mg of NaNO₃.1⁻¹, and in dairy-treated milk (72 samples), from 2.33 to 6.23 mg of NaNO₃.1⁻¹. In the both cases maximal values of nitrates were observed in April, and minimal values in January. With the exception of one pooled sample, all the examined milk samples complied with the Slovak Codex Alimentarius, where the highest values of nitrates and nitrites in some of the final products are fixed. The average value of nitrites in the examined samples of milk taken during the investigated period, amounted to 0.07 mg of NaNO₂.1⁻¹.
3
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Influence of long-term nitrate exposure on calves

100%
Baranova M., Mal'a M., Burdova O., Zezula I.
Bulletin of the Veterinary Institute in Puławy
|
1999
|
tom 43
|
nr 1
A long-term nitrate load was given to calves by oral administration of aqueous solution of KNO₃ to 6 five-week-old experimental calves. The calves were divided into two groups, three animals in each. During a period of six weeks the calves in the experimental group 1, received 2 g of KNO₃ every day, while those of the 2nd group were administered 5 g of KNO₃ per day. After 6 week application, when the weight of the calves reached 75 kg on average, the doses administered were increased to 5 g in the first and 10 g in the second group. The application lasted additional 20 days. The determination of the percentage of methaemoglobin in the blood of the experimental and control calves in the course of the experiment was used to investigate the response of the calves to orally administered nitrate. The mean values of MtHb determined on the last day of administration were lower in both experimental groups of calves than those detected on the first day of the administration of the nitrate (Figs 1-3). The long-term intake of nitrate in a dose of 2-10 g KNO₃, per head daily was not reflected in a deteriorated state of health or in the decreased weight gain of experimental animals in comparison with the control. In order to determine the distribution of the substances administered, the content of residual nitrate and nitrite in the muscles and organs of experimental and control animals was also determined (Figs 4-5).
4

Effect of natural pigment of Monascus purpureus on the organoleptic characters of processed cheeses

88%
Baranova M., Mal'a P., Burdova O., Hadbavny M., Sabolova G.
Bulletin of the Veterinary Institute in Puławy
|
2004
|
tom 48
|
nr 1
5

Hygiene of pasteurized milk depending on psychrotrophic microorganisms

88%
Burdova O., Baranova M., Laukova A., Rozanska H., Rola J. G.
Bulletin of the Veterinary Institute in Puławy
|
2002
|
tom 46
|
nr 2
6
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Variation in quality of yoghurt after addition of nitrates and nitrites into the fermenting medium

88%
Baranova M., Michalski M. M., Burdova O., Mal'a P., Zezula I.
Bulletin of the Veterinary Institute in Puławy
|
1997
|
tom 41
|
nr 2
The changes in titratable acidity of white cream yoghurt were observed during its ageing and storage dependently on the addition of sodium nitrate and nitrite into the fermenting medium. Dynamics of residual nitrates and nitrites was investigated in parallel. Sodium nitrate was added to the yoghurt mixture, obtained directly from the fermentation tank, in Agromilk Sabinov plant, in an amount so that the concentrations in samples were 100, 50, 15, 10 and 5 mg NaNO₃.1⁻¹. Concentrations of nitrites were 100, 50, 10, 5, 1 and 0.5 mg NaNO₂.1⁻¹. Samples of 50 ml volume were incubated in a thermostat at 32°C for 17 h and then stored in a refrigerator at 6°C for 48 h.The highest inhibition, 36.6% of titratable acidity, was observed in yoghurt samples containing 100 mg NaNO₃.1⁻¹, and 54.5% inhibition was detected in samples with the content of 100 mg NaNO₂.1⁻¹ at the 15 th hour of the incubation. The inhibition of titratable acidity in yoghurt sarnples containing 15, 10 and 5 mg NaNO₃.1⁻¹ was small during the incubation and levelled at or slightly exceeded the titratable acidity of control samples during storage. Nitrate residues determined after 48 h of storage in a refrigerator did not exceed in any sample the value of 15 mg NaNO₃.1⁻¹ which is the limit for milk according to the Directives – Hygienic requirements on extraneous matter in comestibles. A decrease in titratable acidity of yoghurt at the end of incubation and during storage was also observed after the addition of 0.5 mg NaNO₂.1⁻¹. Concentrations of 100, 50 and 10 mg NaNO₂.1⁻¹ also affected the consistency of yoghurt, resulting in insufficient thickness. All samples to which nitrite was added contained residual nitrites above the allowed level of 0.1 mg NaNO₂.1⁻¹.
7
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Methemoglobin levels in blood and transrenal passage of nitrates and nitrites in calves

88%
Baranova M., Jackova A., Mal'a P., Burdova O., Zezula I.
Bulletin of the Veterinary Institute in Puławy
|
2000
|
tom 44
|
nr 1
In a study with 12 calves on milk nutrition the course of methemoglobinemia as well as transrenal passage of nitrates and nitrites after a single per os administrations of 4 g NaNO₂ per animal and 30 g KNO₃ per animal in the form of water solutions has been observed. The response of the organism of calves to per os administered doses of sodium nitrite and potassium nitrate was observed by the determination of the methemoglobin percentage in blood and the nitrate and nitrite content in urine before the administration of the respective dose and after 1, 2, 3 and 4 h after the administration. A significant elevation in the values of methemoglobin was recorded 2 h after the administration of 4 g of NaNO₂ per animal. The mean value of methemoglobin in blood was 18.84% of total hemoglobin. A slight decline in the values occurred as early as 3 h after the administration. Of clinical signs, cyanosis of visible mucosae was observed. The highest nitrite and nitrate values in urine were determined 2 h after per os administration of 4 g of NaNO₂. With the administration of 30 g KNO₃ per animal, the most pronounced elevation in methemoglobinemia was observed after 3 h, when the mean values of methemoglobin was 11.75%. Of clinical signs, only slight cyanosis of mucosae was detectable. Maximum values of nitrates in urine of experimental calves 3 h after the administration of 30 g KNO₃ per animal, with the mean value of 29.9 mM NO₃⁻ clearly demonstrate a good transrenal passage of nitrates in calves on milk nutrition.
8
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Depletion of tylosin from the mammary gland of mastitic cows and its influence on the qualitative properties of milk

76%
Dudrikova E., Pistl J., Sokol J., Nagy J., Burdova O., Puzder M.
Bulletin of the Veterinary Institute in Puławy
|
2000
|
tom 44
|
nr 2
Six cows suffering from mastitis were injected intramuscularly with tylosin at a dose of 10 mg kg⁻¹ b.w. for five consecutive days after the morning milking. The milk samples were collected for 13 days at 12 h intervals, and from the afternoon and morning milkings were pooled together (1:1). The samples were analysed by liquid chromatography and yoghurt inhibitor test for a detection of tylosin residues. Qualitative parameters of milk (active acidity, somatic cell count, total bacterial count) were also investigated. It was found that the HPLC method and yoghurt inhibitor test gave comparable results. The somatic cell count was above 400 000 per ml of milk. Staphylococcus aureus and pathogenic streptococci were detected in milk from mastitic cows.
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