Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 6

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

The role of astrocytes in the physiology and pathology of the central nervous system

100%
Markiewicz I., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2006
|
tom 66
|
nr 4
Astrocytes are the main class of neuroglia, serving a wide range of adaptive functions in the mammalian nervous system. They interact with neurons, providing structural, metabolic and trophic support for them. In pathological circumstances, astrocytes have the potential to induce neuronal dysfunction, but they can also play a neuroprotective role, releasing neuronal growth factors. Here we review recent findings regarding the role of astrocytes in the biology of the brain in physiological conditions, as well as their reaction following the onset of neurodegenerative disorders.
2

Induced glia activation is harmful for neurogenesis of human umbilical cord blood neural stem cells

81%
Markiewicz I., Winiarska H., Domanska-Janik K., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2007
|
tom 67
|
nr 3
3

Activation of astrocytes and microglia provides the critical cues for HUCB-NSC proliferation and differentiation in vitro

81%
Markiewicz I., Winiarska H., Domanska-Janik K., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 1
Brain infl ammation contributes to the propagation of neuropathological events that involves activation of astrocytes and microglia. It remains obscure how activated glial cells affect the survival and differentiation of neural stem cells (NSC). The aim of the study was to analyze neuronal commitment of Human Umbilical Cord Blood derived Neural Stem Cells (HUCB-NSC) cultured in the presence of normal and LPS- or TMT-activated glial cells. Methods: HUCB-NSC (5 × 104/cm2 ) were co-cultured with normal or LPS (0.1 μg/ ml) and TMT (1μM)-stimulated astrocytes and microglial cells isolated from neonatal rat brain for proliferation and cell phenotype assessment. Pro-infl ammatory cytokines were estimated (ELISA). Results: Normal rat astrocytes induce HUCB-NSC to differentiate mostly into neurones but microglia stimulate HUCB-NSC to differentiate into neurons as well as into astrocytes. LPS- and TMTinduced astrocytes diminish neurogenesis of HUCB-NSC and increase astrocyte differentiation in comparison to non-stimulated astrocytes. Microglia activation by LPS and TMT decreases HUCBNSC differentiation into neurons but enhances oligodendrogenesis compared to normal microglia. Stimulation of astrocytes and microglia by LPS and TMT declines HUCB-NSC proliferation cocultured with astrocytes or with microglia. The presence of IL-1β, IL-6, TNF-α and NO was observed in glia cell culture supernatants after LPS and TMT implementation. Conclusion: Activation of astrocytes and microglia induced by LPS and TMT attenuate pro-neural effect of non-stimulated (resting) glia and suppress proliferation of HUCB-NSC in vitro. The release of pro-infl ammatory cytokines and NO might be partly responsible for this effect. Supported by MSHE grant No 142/P01/2008/35
4

The expression of the regulatory factors MyoD and Myf-5 in myogenic stem cells during differentiation in vitro

80%
Domek K., Markiewicz I., Moraczewski J.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
5

Transplantation of neural stem cells derived from human cord blood to the brain of adult and neonatal rats

71%
Jablonska A., Kozlowska H., Markiewicz I., Domanska-Janik K., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2010
|
tom 70
|
nr 4
Searching for a reliable source of alternative neural stem cells for experimental treatment of neurological disorders we have established neural stem cell line derived from human umbilical cord blood (HUCB-NSC) (Buzanska et al. 2006). These cells have been shown to differentiate along neuronal and glial lineages in the promoting in vitro conditions. In the current study we transplanted HUCB-NSC into rat brain to determine whether the neural progenitors would be able to survive, migrate and eventually adopt neural phenotypes after exposure to central nervous system (CNS) microenvironment. Our experiments revealed that HUCB-NSC grafting into the brain of adult rats limited their survival up-to two weeks probably due to their elimination by severe immunological host reaction evoked by xenotransplantation. HUCB-NSC graft in neonates survived longer time in rat brain, migrated, proliferated and differentiated into neuronal cells however their presence in the host tissue did not exceed more than five weeks after transplantation.
6

Analysis of the neurogenic potential of human umbilical cord blood neural stem cells (HUCB-NSC) after their transplantation into rat brain

51%
Kozlowska H., Markiewicz I., Jablonska A., Habich A., Zychowicz M., Wanacka E., Domanska-Janik K., Lukomska B.
Acta Neurobiologiae Experimentalis
|
2009
|
tom 69
|
nr 1
Transplantation (tx) of neural stem cells (NSC) is the key strategy of cell replacement therapy in the central nervous system. The goal of the study was to compare survival, migration and differentiation of HUCB-NSC after their tx into the brain of neonatal and adult Wistar rats. Methods: HUCB-NSC (2 × 104 ) labeled with CMFDA were tx into SVZ of the postnatal day 0 (P0) rats or into intact brain of adult rats. After 1, 3, 7, 14 or 21 days brains were removed, frozen and cut into 20 μm coronary slices, then immunohistochemical studies were performed to visualize HUCB-NSC fate in the brain. Results: In neonatal rats, 3 days after tx most of HUCB-NSC remained in the graft. During the fi rst week HUCB-NSC started to disperse and migrate. HUCB-NSC situated at the periphery of the graft or in migratory stream display proliferation marker (Ki67). After 7ñ21 days HUCB-NSC survived in the host brain with many cells expressing neuronal or astrocytic phenotypes. Few of HUCB-NSC presented the features of adult neurons (MAP2+ with long protrusions). In adult rats, 3 days after tx, HUCB-NSC form dense deposit with single cells migrating into brain tissue. Most of the HUCB-NSCs stayed undifferentiated with few cells expressing neuronal (NF200) or astrocytic (GFAP) markers. After 7 days numerous HUCB-NSC situated inside the graft underwent degeneration and subsequent depletion. Tx HUCB-NSC induced infl ammatory response detected by macrophage/microglia (ED1+) accumulation and astrogliosis (GFAP+). No viable HUCB-NSC were found after 14 days. Conclusions: Host environment dictates the fate of tx neural stem cells derived from human cord blood however immunological response in adult rats limits the time of observation due to short survival of HUCB-NSC. Supported by MSHE grant no 2PO5A05430
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.