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The aim of these studies was to assess the effect of chemical conservants (FA = formic acid, PA = propionic acid, FPA+i = formic acid, propionic acid and ammonium ions) on the cell counts of yeasts and mould fungi in silage. The silage was prepared from corn (Zea mays L), cultivar Buran FAO (240). The effect of the applied conservants on silage aerobic stability was also assessed. The performed chemical analyses comprised the determination of: the content of dry matter (DM), lactic acid (LA), acetic acid (AA), ethanol, water soluble sugars (WSC), crude protein (CP) and pH. The applied preparations were found to reduce the number of yeast and mould fungi cells in all the examined silages. The growth of fungi was inhibited most strongly by the FPA+i preparation (containing a mixture of formic and propionic acids and ammonium ions). The yeast cell counts dropped (P«0.05) from 8.50 107 CFU g-1 silage in the control (CCS = control corn silage) to 2.60 107 CFU g' in silage treated with FPA+i, whereas counts of the mould fungi cells - from 15.20 104 CFU g-1 silage in the control to 4.60 104 CFU g-1 in silage treated with FPA+i. The applied conservants increased (P«0.05) the content of DM from 255.00 g • kg 1 in control to 266.60 g • kg-1 with PA, WSC from 27.10 g • kg 'DM to 30.50 g • kg -1DM with FPA+i and ethanol from 9.10 g • kg 'DM to 11.21 g • kg -1DM with FPA+i in the silage. The additives decreased concentrations of lactic acid, acetic acid and crude protein concentration after opening the barrels. The diversifying factors decreased the pH value in the examined silage. The experimental conservants were found to improve the aerobic stability of silages after 7 days of air exposure.
The objective of the performed investigations was to isolate pathogenic fungi from contaminated maize cobs, to assess the appearance of maize cob fusariosis and to determine grain contamination with deoxynivalenol in the cultivation of genetically modified maize containing a gene resistance against European corn borer (Ostrinia nubilalis Hbn) as well as selected non-modified cultivars. The plant material comprised the following genetically modified maize cultivar: DKC 3421 YG (MON 810) and non-modified cultivars obtained from Smolice Plant Breeding Ltd., IHAR Group: Junak (FAO 210–220), Prosna (FAO 220), SMH (FAO 230), Baca (FAO 220). Prior to harvesting, the occurrence of maize cob fusariosis was determined in the 89 (BBCH) developmental ripening stage. Microbiological assessment was carried out on grains selected from cobs characterized by various pathological symptoms. In 2008, a total of 133 isolates was obtained from the examined samples of infected maize plants, of which 51 isolates were species-identified, while in 2009, the total of 123 isolates were determined, of which 63 were species-identified. In both experimental years, the majority of isolates contained fungi from the Fusarium genus. The performed analysis of mean levels of cob contamination by fusarioses revealed that DKC 3421 YG (MON 810) and SMH (FAO 230) cultivars showed the smallest levels of contamination as well as the lowest percent of cob contamination per plant, while Junak (FAO 210–220) and Baca (FAO 220) cultivars were characterized by the highest degree of contamination. The lowest deoxynivalenol concentrations were determined in years 2008 and 2009 in the case of the DKC 3421 YG (MON 810) cultivar, whereas Prosna (FAO 220) cultivar was characterized by the highest deoxynivalenol concentration.
The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg-1 biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (P<0.05) numbers of Clostridium, Enterobacteriaceae, yeasts and mold fungi cells, and increase (P<0.05) the number of LAB (lactic acid bacteria) in comparison with the control in both silages. Chemical analysis of the maize silage showed that the biological additive caused an increase (P<0.05) in DM (dry matter), CP (crude protein), WSC (water soluble carbohydrates), LA (lactic acid), AA (acetic acid), ethanol, and a decrease (P<0.05) in the concentration of BA (butyric acid), N-NH3 and pH value in comparison with the control. Chemical analysis of silage samples from the grass-legume mixture showed that the additive caused an increase (P<0.05) in the content of DM, CP, WSC, LA and AA in comparison with the control. Samples of silage with the addition of an inoculant were characterized by a lower (P<0.05) content of BA, N-NH3, ethanol and pH value. The biological additive impoved the aerobic stability of silages in the aerobic phase.
This research was carried out to determine the effects of bacterial silage inoculant using as silage additives on the fermentation charakteristics, cell wall contents and aerobic stability of maize silages. Maize silage was harvested in the 89 (BBCH) developmental ripening stage. Biological additive was used as additive which contains Enterococcus faecium PCM 1858, Pediococcus acidilactici PAL-34, Lactobacillus plantarum PCM 493, Lactobacillus buchneri DSMZ 5987, Lactobacillus rhamnosus PCM 489, Lactobacillus brevis PCM 488, Lactobacillus lactis PCM 2379. Maize was ensiled in 4 dm3 special PCV laboratory microsilos with a cover permitting gaseous products. The microsilos were stored at 10–15±2şC undr laboratory conditions. Microsilos from each group were sampled for microbiological and chemical analyses on the days 3, 14, 21 and 60 after ensiling, whereas aerobic stability was determined after 7 days. As a result, bacterial silage inoculant improved fermentation, decreased cell wall contents, deoxynivalenol (DON) concenration, do not improved aerobic stability of maize silage after 7 days exposure to air.
Doświadczenie polowe przeprowadzono w latach 2005-2007 w Zakładzie Doś¬wiadczalno-Dydaktycznym w Gorzyniu należącym do Uniwersytetu Przyrodniczego w Poznaniu. Czynnikami badawczymi były: dwa terminy zbioru zielonki z łubinu żółtego odmiany ‘Parys’ (I – w fazie płaskiego strąka i II – w fazie dojrzałości zielonej nasion) oraz dodatki kiszonkarskie (biologiczny, chemiczny) i kontrola bez dodatku. Skład mikrobiologiczny kiszonek był zróżnicowany w poszczególnych latach badań. Kiszonki z zielonki zbieranej w fazie płaskiego strąka (I termin zbioru) zawierały istotnie więcej bakterii kwasu mlekowego, a mniej drożdży i Clostridium. Konserwant biologiczny powodował istotny wzrost zawartości bakterii kwasu mlekowego. Oba konserwanty wpłynęły na istotny spadek niekorzystnych bakterii z rodziny Enterobacteriaceae, Clostridium, drożdży i grzybów pleśniowych, przy czym preparat chemiczny był bardziej skuteczny niż biologiczny. W badaniach nie stwierdzono istotnego wpływu terminów zbioru zielonki na skład chemiczny kiszonek, a zastosowane konserwanty modyfikowały zawartość kwasu mlekowego, amoniaku, cukrów redukujących i pH kiszonki.
In the present study, the presence of flaA, cadF, cdtB, and iam genes of Campylobacter sp. were analysed using PCR. Material for analyses comprised 100 Campylobacter sp. isolates obtained from healthy broiler chickens, fatteners, and calves, among which 84 isolates were ascribed to Campylobacter jejeuni and 16 to Campylobacter coli. All isolates (100%) had the cadF gene responsible for adhesion and the flaA gene determining the motility of the analysed bacteria. The frequency of occurrence of the cdtB gene responsible for the production of the cytolethal distending toxin (CDT) was determined to be high (98.6% in broiler chickens, 75% in fatteners, 62.5% in calves). In case of the iam gene, the highest frequency was recorded in Campylobacter sp. isolated from broiler chickens (84.7%), while in strains collected from fatteners and calves it was lower, amounting to 41.7% and 18.8%, respectively.
The aim of the study was to ascertain the impact of irrigation or its absence on respiratory and dehydrogenase activities in soils under maize growth. Investigations were carried out in years 2007-2009 at six times of analysis during the maize growth season. The experiment included experimental plots on which maize of Clarica hybrid (FAO 220, Pioneer) intended for CCM was grown. The experimental plots were irrigated with the use of semi-permanent sprinkling machines. Dehydrogenase activity in the soil was determined colorimetrically, and soil respiratory activity was determined with the titration method. Soil respiratory activity did not exhibit significant variability during the growth season with the exception of 2009 at the second time of analysis (at the 2-3 maize leaf stage), when the highest average values in the season were recorded. In the third year of the analysis (2009), practically speaking, no significant differences were observed in the activity of the examined enzymes between irrigated and nonirrigated plots. The comparison of the dates of the analyses showed that the highest activity of the investigated enzymes occurred in the spring before sowing and in the summer. Mean values of the respiratory activity did not show large differences in the individual seasons.
The investigations comprised 100 piglets of crossbreed Polish Landrace x Large White Polish breed. Faeces samples were collected on the 2nd d of piglets' life (control). On the 5th d of life of the piglets, probiotic paste was applied and 7 d later, faecal samples were collected again. The material included 100 isolates of Campylobacter sp. obtained from healthy piglets. All isolates were assigned to the Campylobacter coli species. The occurrence of virulence genes was determined by the PCR method. Drug- resistance of the obtained isolates was determined using diffusion tests and E-test strips. All isolates deriving from the control group piglets were found to contain the cαdF gene responsible for adhesion, as well as, gene flaA influencing motility of the examined bacteria. In piglets fed diets supplemented with probiotics, the cαdF gene occurred in 100% isolates and gene flαA - in 99% isolates. Campylobacter coli isolates obtained from piglets from the control group exhibited the highest resistance with respect to ciprofloxacin and enrofloxacin. The similar results were recorded in the case of isolates obtained after the probiotic application. The majority of the isolates generated α type haemolysis (91%-92%). No significant differences were recorded in the capability of generating haemolysis between isolates obtained before probiotic administration and the isolates obtained after the application of the experimental probiotic.
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